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All Journal SIMBIOSIS
Alayna Lillahida Indri Himawan
Universitas Udayana
Agriculture, Biological Sciences & Forestry

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STRATEGIES TO IMPROVE THE QUALITY OF BIOPHARMACEUTICALS IN CHINESE HAMSTER OVARY (CHO) MAMMALIAN CELLS: A LITERATURE REVIEW Alayna Lillahida Indri Himawan; Adi Santoso; Endah Puji Septisetyani; Pekik Wiji Prasetyaningrum; Nurul Maulida Rahma; Inna Narayani
SIMBIOSIS Vol 11 No 2 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i02.p11

Abstract

Chinese hamster ovary (CHO) cells are essential cells frequently used to produce biopharmaceutical molecules, especially glycoproteins. Nevertheless, to obtain an efficient production process and effective therapeutic consistency, the right strategy is needed to improve the quality of biological drug products. It is known that all the critical molecules involved in the immune response are glycoproteins, and many therapeutic proteins, such as vaccines, antibodies, and enzymes, require glycans to have total biologic activity. This review discusses the strategy used in CHO cells to modulate sialylation patterns through overexpression of sialyltransferases, CMP-sialic acid transporter, UDP-GlcNAc 2-epimerase (GNE), ManNAc kinase (MNK), and other related enzymes. In addition to modulating biosynthetic pathways to increase sialic acid content with gene overexpression techniques, this review includes methods for inserting glycosylation sites and manipulating glycans to produce the desired glycoforms. An approach through protein fusion techniques with Fc molecules from human IgG is also discussed to increase the protein half-life. Finally, as a strategy to improve the quality of biopharmaceutical molecules, this review also discusses the importance of cell monoclonalization in developing a cell line that truly originates from a single clone. This is a very critical step to obtain batch to batch consistency during the production process of a biopharmaceutical molecule.
Co-Authors Adi Santoso Endah Puji Septisetyani Inna Narayani Nurul Maulida Rahma Pekik Wiji Prasetyaningrum