Article Per Year (5 Year)
p-Index From 2019 - 2024
1.909
P-Index
This Author published in this journals
All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana SIMBIOSIS Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Journal of Health Sciences and Medicine
Inna Narayani
Program Studi Biologi FMIPA Universitas Udayana Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology

Published : 22 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 22 Documents
Search

 1   2   3 
THE EFFECTS OF DIETARY INCLUSION OF DETOXIFIED Leucaena leucocephala LEAF MEAL ON THYROIDAL ACTIVITY OF RATS DURING GESTATION-LACTATION PERIOD Ngurah Intan Wiratmini; Inna Narayani; Ni Luh Eka Setiasih
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.757 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p02

Abstract

Leucaena leucocephala provides the highest amount of proteins compared to other greens. However, its use is restricted due to the content of mimosine, an antinutrition non-protein amino acid known to be toxic to animals. This study aimed to observe the effect of administration of more than 10% of processed Leucaena leucocephala leaf meal to the level of T3, T4 and the thyroid histopathology. In this study, Leucaena leucocephala leaves were soaked in distilled water for 12 hours. The processed of Leucaena leucocephala leaf meal was made into pellets containing 0%, 7.5%, 15%, and 22.5% leaf meal of total feeds respectively, and was fed to the rats during pregnancy and lactation. The level of triiodothyronine (T3) and thyroxine (T4), and the thyroid histological features were the parameters observed. The collected data were statistically analyzed by SPSS 20.0 for Windows using One-way ANOVA followed by Duncan Multiple Range Test to observe any significant difference among the 4 treatment groups (?=5%). Meanwhile, the presence of hyperplastic cells and follicle lumens filled with vesicles and colloids was descriptively analyzed by means of available literatures. The analysis of T3 and T4 level revealed that there was no any significant difference between the control and treatment groups. The epithelial cells of thyroid follicles in the treatment groups of P1, P2 and P3 showed hyperplasia and were detached from their respective basal membranes.
CD4 COUNT FROM CRYOPRESERVATION OF BUFFY COAT AND PBMC Rasmaya Niruri; Inna Narayani; Wayan Tunas Artama; Mantik Astawa; Ahmad Hamim Sadewa
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.785 KB)

Abstract

This study aimed to determine CD4 count from cryopreservation of Buffy coat (BC) and PeripheralBlood Mononuclear Cell (PBMC) with and without ficoll. Fifteen EDTA Blood sample (2 ml for eachtube) were drawn from one adult healthy subject. The samples were categorized into five group beforemeasuring the CD4 level (which were fresh whole blood [Group(G)-I], BC without ficoll [fresh <GII>and frozen <G-III>] , and PBMC resulted from BC and ficoll isolation [fresh <G-IV> andfrozen <G-V>]. Each group was replicated three times. Blood storage before preparation was less thanfour hours. Two months cryopreservation using liquid nitrogen (in 40% FBS, 10% DMSO, and RPMI)was conducted. The mean value of CD4 count (cell /mu1) were 522 (G-I), 1410 (G-II), 906 (G-III), 807(G-IV), and 733 (G-V). CD4 count, after 2 month preservation in liquid nitrogen, of the BC sample (G-III) was higher (906 cell /mu1) than PBMC (G-IV) sample (733 cell /mu1).
EFFECT OF RESTING TIME ON PERIPHERAL BLOOD MONONUCLEAR CELL YIELDS Inna Narayani; Rasmaya Niruri; Nyoman Mantik Astawa
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.459 KB)

Abstract

Cryopreservation of PBMC (peripheral blood mononuclear cells) was done to preserve and analyze the number of PBMC derived from blood samples which come in at different time. The batch analysis wasperformed at the same time in order to reduce variations in results. The analysis on the cells numbers carried out after 1, 3, 6, 12, and 24 hours. Heparinized whole blood was collected from healthy subject by venipuncture, and stored at room temperature. Blood is processed by centrifugation in Ficoll density gradient following the established method of Balai Besar Veteriner Denpasar. Buffy coat layer was collected and washed twice with HBSS (Hank's balanced salt solution) and was counted in Turk’s solution. The cells were then dissolved in 1 ml of cold freezing medium containing 10% DMSO and 50% FBS (fetal bovine serum) and stored overnight at -80°C before storage in liquid nitrogen vessel for few weeks. The samples rapidly thawed in a water bath at 37°C and washed twice with PBS (phosphate buffered saline). The cells were stored in 4°C PBS and counted in Turk’s solution after 1, 3, 6, 12, and 24 hours. The results obtained were varied with a declining trend.
Morfologi dan Perkembangan Skeleton Fetus Tikus (Rattus norvegicus L.) yang Diberi Pakan Mengandung Kulit Nanas (Ananas comosus Merr.) selama Kebuntingan Ni Komang Frely Astuti; Iriani Setyawati; Inna Narayani
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p20

Abstract

Pineapple peel in animal feed might contain bromelain enzyme which could distrupt pregnancy in animal laboratory. This experiment used a complete randomized design with 16 pregnant rats which were divided into four treatment groups. The treatments are feed containing 0 (K); 4,35 (P1); 8,70 (P2); and 13,05 (P3) g/rat/day of pineapple peel that were given during pregnancy. Parameters of morphological fetus observed were alive fetuses, weight, length, and fetal abnormalities and skeleton development i.e. axial skeleton (costal, sternebral, and vertebral bones) and appendicular skeleton (vertebra caudal, metacarpal and metatarsal bones). Data were analyzed by SPSS version 22, using One Way Anova or Kruskal Wallis. The result showed that fetuses from pregnant rats which administered with feed containing pineapple peel showed teratogenic effect. Fetal morphological abnormalities of the fetus is subcutaneous hemorrhage in were found at dose of 8,70 (P2) and dose of 13,05 (P3) g/rat/day. Feed containing pineapple peel could inhibit ossification of apendicular skeleton in vertebra caudal, metacarpal, and metatarsal.
TEKNIK PERANCANGAN PRIMER UNTUK SEKUEN GEN MDR-1 VARIAN 1199 PADA SAMPEL BUFFY COAT PASIEN ANAK DENGAN LLA Putu Desy Yustinadewi; Putu Sanna Yustiantara; Inna Narayani
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p16

Abstract

Single Nucleotide Polymorphism (SNP) 1199 dapat diidentifikasi menggunakan sampel buffy coat dengan metode Polymerase Chain Reaction (PCR). Komponen- komponen yang diperlukan pada proses PCR adalah template DNA; sepasang primer, yaitu suatu oligonukleotida pendek yang mempunyai urutan nukleotida yang komplementer dengan urutan nukleotida DNA templat; dNTPs (Deoxynucleotide triphosphates); buffer PCR; magnesium klorida (MgCl2) dan enzim polimerase DNA (Handoyo dan Rudiretna, 2001). Primer sangat mempengaruhi spesifitas dan sensitivitas reaksi PCR. Rancangan suatu primer merupakan salah satu parameter penentu keberhasilan suatu proses PCR (Ebd-Elsalam, 2003). Primer untuk sekuensing gen MDR-1 variant 1199 berhasil didesain dalam kondisi terbaik. Panjang sekuen primer forward sejumlah 21 oligonukleotida dan reverse sejumlah 20 oligonukleotida dengan fragmen sebesar 225 pb.
Monitoring Proviral DNA Of Jembrana Disease Virus In Bali Cattle Using PCR Ni Putu Eka Krisnayanti; Made Pharmawati; Inna Narayani; Ni Luh Putu Agustini
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p03

Abstract

The development of biotechnology in molecular field make it possible to detect the presence of a disease earlier, faster and more accurately. One of the technique in biotechnology which has been highly developed and often be used in biological and medical research is PCR method. This molecular technique allows to accurately identify the DNA level in presence of a particular disease. This study aims to do monitoring the presence of proviral DNA Jembrana Disease Virus in bali cattle from all regencies in Bali. The blood samples of bali cattle is taken from all regencies in Bali in 2017 as many 170 samples and in 2018 from Buleleng Regency there are 101 samples and form Karangasem Regency 152 samples. DNA is extracted by QIAamp DNA blood mini kit and samples from 2017 is made to 34 pool samples, whereas samples from 2018 is made to 20 pool samples (Buleleng Regency) and 30 pool samples (Karangasem Regency). PCR is conducted by JDV 1 primer and JDV 3 primer in total reaction 25 µL with 35 cycles. PCR is also conducted on positive control and negative control. The result indicate that 34 pool samples of bali cattle from 2017 or 20 pool samples and 30 pool samples from 2018 is not indicate the presence of proviral DNA Jembrana Disease Virus. Keyword: PCR, proviral DNA, Jembrana Disease Virus
Deteksi Level Antibodi Pada Serum Darah Anjing Kintamani Pasca Vaksinasi Rabies dengan Direct ELISA I Putu Agus Tirta Cahyana; Made Pharmawati; Inna Narayani
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p19

Abstract

The success of rabies vaccination is characterized by the growth of seropositive (?0,5 IU) antibody titers after vaccination. One of the tests carried out to monitor antibody growth is ELISA (Enzyme Linked Immunosorbent Assay). This study aims to determine the effect of different vaccine doses on rabies antibody growth in kintamani dogs. The study was conducted at Perum Taman Graha, Jimbaran and Bali Besar Veteriner (BBVet) Denpasar, Bali. The design of the study used 2 variables, namely differences in vaccine doses (0.5 and 0.75 cc) and blood collection times (1, 2 and 3 months after vaccination). The results showed that vaccine dose of 0.5 cc produced higher antibody titers than vaccine dose of 0.75 cc, however it was not significantly different based on t-test. Key word : Antibody, ELISA, Rabies
Aplikasi fungi mikoriza arbuskula Glomus sp. dan Trichoderma sp. sebagai pupuk hayati dan biostimulator pertumbuhan tanaman tomat (Solanum lycopersicum L.) Anak Agung Yulia Anggiani; Meitini Wahyuni Proborini; I Ketut Muksin; Inna Narayani
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p02

Abstract

Tomato (Solanum lycopersicum L.) is a horticultural crop that has many benefits and potential to be developed. Market demand for tomatoes is increasing, but tomato production in Bali has decreased due to biotic and abiotic factors. One of efforts to increase tomato production is by using the Mycorrhizal Arbuscular Fungi (FMA) Glomus sp. and Trichoderma sp. which act as a biofertilizer and biostimulator. The study was conducted in November 2020 until February 2021 and aims to analyze the effect of AMF, Trichoderma, and combinations on the growth of tomato plants. Research site was at the Greenhouse and the Laboratory of Plant Taxonomy in the Department of Biology Faculty of Mathematics and Natural Sciences Udayana University. The experiment used factorial Randomized Block Design (RBD) with two factors, three replicates. First factor was the dose of AMF Glomus (0, 100, 150, and 200 spores per plant) and the second factor was the dose of Trichoderma (0, 10, and 20 mL per plant). Data of this study was analyzed using Analysis of Variance (ANOVA) followed by Duncan Multiple Range Test (DMRT). The result showed that AMF Glomus 150 spores increased plant height, root length, number of flower bunches, dry plant mass, and obtained the highest AMF colonization percentage at 35% (medium category).
VARIASI GENETIK SUKU BATAK YANG TINGGAL DI KOTA DENPASAR DAN KABUPATEN BADUNG BERDASARKAN TIGA LOKUS MIKROSATELIT DNA AUTOSOM YOSSY CAROLINA UNADI; INNA NARAYANI; I KETUT JUNITHA
Jurnal Biologi Udayana Vol 14 No 2 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.221 KB)

Abstract

Study of genetic variation on three microsatellite DNA loci D2S1338, D13S317 and D16S539 from 76 unrelated individuals of Batak ethnic who live in Denpasar city and Badung regency were analyzed by using PCR method (SuperMix Kit, Invitrogen). DNA samples were extracted from blood cell using phenol-chloroform method and ethanol precipitation. The result of this study found 14 alleles on D2S1338 locus, 10 alleles on D13S317 locus and eight alleles on D16S539 locus. The finding showed high genetic diversity on three loci as well as on three sub-ethnic Batak of Batak Toba, Karo and Simalungun which were 0.8637; 0.7314 and 0.7692 respectively.
Potensi Streptomyces sp. dalam menghambat pertumbuhan jamur Colletotrichum acutatum pada cabai merah besar (Capsicum annum L.) secara in vitro Rochmalia Juniarti Putri; Retno Kawuri; Anak Agung Ketut Darmadi; Inna Narayani
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p12

Abstract

Red chilli plant (Capsicum annum L.) is one of the most popular vegetable crops in Indonesian society. One of them the pathogens attacks is Colletotrichum acutatum, a fungus causing anthracnose on red chilli. This study aims to determine the existence of Streptomyces sp. bacteria in the rhizosphere of the red chilli plant; the ability of Streptomyces sp. in inhibiting C. acutatum; Minimum Inhibitory Concentration (MIC) of Streptomyces isolates extracts in inhibiting C. acutatum; The Streptomyces isolation was carried out by dilution method using selective meida, namely Yeast Malt Agar. The Dual Culture method was used as an inhibition test between Streptomyces sp. and C. acutatum in vitro. A well diffusion method was used to test the effectiveness of the Streptomyces sp. and MIC filtrate concentration in inhibiting C. acutatum. The data obtained in this study were analyzed with Analysis of Varian (ANOVA) then continued with Duncan Multiple Range Test with 5% significance. Five Streptomyces isolates were found, namely Streptomyces sp.1, Streptomyces sp.2, Streptomyces sp.3, Streptomyces sp.4, and Streptomyces sp.5 in the rhizosphere of healthy C. annum L. plants in Daup Village, Kintamani District, Bangli Regency. Streptomyces sp. isolates. can significantly inhibit the growth of the fungus C. acuatum with inhibitory power ranging from 50.30% to 83.76%, Streptomyces sp.5 isolate was able to provide the highest percentage of inhibition in C. acutatum of 83.76 ± 2.91% with MIC 7% (v/v) with a diameter of 6.40 mm.
Co-Authors A.A.S. A. Sukmaningsih Adi Santoso Ahmad Hamim Sadewa Alayna Lillahida Indri Himawan Anak Agung Ketut Darmadi Anak Agung Yulia Anggiani Christian Subagya Gunardi Endah Puji Septisetyani Fakhrurrasi Fakhri Galih Anindita Kusumatjahja Haryo Seto Wicaksono I Gusti Ngurah Kade Mahardika I Ketut Junitha I KETUT MUKSIN I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Putu Agus Tirta Cahyana I Wayan Arya Mahardika Iriani Setyawati Iriani Setyawati Ketut Ariawati Lucia Emy Octavia M Luthfi Ardiansyah MADE PHARMAWATI Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Komang Frely Astuti Ni Luh Eka Setiasih Ni Luh Putu Agustini Ni Putu Eka Krisnayanti Nurul Maulida Rahma Pekik Wiji Prasetyaningrum Putu Desy Yustinadewi R. Niiruri Rasmaya Niruri Rasmaya Niruri Retno Kawuri Revi Juniar Nutrihidayah Rochmalia Juniarti Putri S. Herawati Wayan Tunas Artama YOSSY CAROLINA UNADI Yustiantara, Putu Sanna