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All Journal Malacca Pharmaceutics Grimsa Journal of Science Engineering and Technology
Trina Ekawati Tallei
Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Immunology & microbiology Medicine & Pharmacology Physics

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In Vitro Antioxidant Activity of Chrysanthemum indicum Flowers Extract and Its Fraction Jeclin Inebel Dolongtelide; Fatimawali Fatimawali; Trina Ekawati Tallei; Elly Juliana Suoth; Herny Emma Inonta Simbala; Irma Antasionasti; Marko Jeremia Kalalo
Malacca Pharmaceutics Vol. 1 No. 2 (2023): October 2023
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/mp.v1i2.26

Abstract

Chrysanthemum indicum L., commonly known as Chrysanthemum flower, is an herbaceous plant that has a long-established history of medicinal usage. There has been extensive research about C. indicium L, especially about its antioxidant activities, but not much has been done on its fraction. This study aimed to explore the efficacy of the ethanol extract and its fraction derived from Chrysanthemum flowers in scavenging free radicals. The antioxidant potential of the ethanol extract, as well as its aqueous and n-hexane fractions, was evaluated using the 2,2-diphenyl-1-picrilhidrazine (DPPH) method in vitro. The degree of antioxidant activity was quantified by determining the IC50 value, which corresponds to the concentration of the extract or fractions required to inhibit 50% of DPPH free radicals. The results obtained from this investigation provide strong evidence that the ethanolic extract, as well as its aqueous and n-hexane fractions, exhibited significant antioxidant activity. The measured IC50 values for the ethanolic extract, aqueous fraction, and n-hexane fraction were 1.350 µg/mL, 1.109 µg/mL, and 7.588 µg/mL, respectively.
Evaluation of Antibacterial Properties from Endophytic Fungi of Chrysanthemum indicum (L.) Flowers against Escherichia coli and Staphylococcus aureus Titah Amelia Ratte; Fatimawali Fatimawali; Trina Ekawati Tallei; Elly Juliana Suoth; Irma Antasionasti; Paulina Yamlean
Grimsa Journal of Science Engineering and Technology Vol. 1 No. 2 (2023): December 2023
Publisher : Graha Primera Saintifika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61975/gjset.v1i2.15

Abstract

Uncovering the therapeutic potential of secondary metabolites produced by plants, animals, and microbes constitutes the foundation for the development of novel medications. The objective of this investigation is to discern the classes of secondary metabolites and assess the antibacterial properties of endophytic fungal extracts obtained from Chrysanthemum indicum L. flowers. Through the isolation process, five isolates designated as JEC1, JEC2, JEC3, JEC4, and JEC5 were identified. The cultivation of endophytic fungal isolates spanned a three-week period before undergoing extraction with ethyl acetate. The phytochemical tests revealed the presence of alkaloids, flavonoids, steroids, terpenoids, saponins, and tannins in the ethyl acetate extract. Antibacterial activity was determined using the agar well diffusion method, with ciprofloxacin serving as a positive control. Notably, all ethyl acetate extracts from endophytic fungi exhibited antibacterial activity. The most substantial inhibitory diameter against Staphylococcus aureus was recorded as 19.1±0.8 mm for the JEC3 endophytic fungi, while Escherichia coli exhibited an inhibitory diameter of 16±1.1 mm for the JEC2 endophytic fungi.
Co-Authors Elly Juliana Suoth Fatimawali . Herny Emma Inonta Simbala Irma Antasionasti Jeclin Inebel Dolongtelide Marko Jeremia Kalalo Paulina Yamlean Titah Amelia Ratte