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All Journal Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Biogenesis: Jurnal Ilmiah Biologi Techno: Jurnal Penelitian
Abdullah, Abubakar
Pendidikan Biologi, Universitas Khairun, Ternate
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Education Immunology & microbiology

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Variabilitas Genetik Ornithoptera croesus lydius Kupu-Kupu Endemik Pulau Halmahera Berbasis Data Molekuler PCR-RAPD Abdu Mas'ud; Abubakar Abdullah; Chumidach Roini
Biogenesis: Jurnal Ilmiah Biologi Vol 6 No 1 (2018)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v6i1.4173

Abstract

Ornithoptera croesus lydius is one of endemic butterlfy in Halmahera island North Maluku. It is macrolepidotera tipe, with interesting colour body at male and female butterfly. At Ornithoptera croesus lydius male and female butterfly have variation morphology size and colour body. The genetic variability on the  O.c lydius  butterflies is level intraspecies variability. The objective of the research is to know the genetic variability on the O.c lydius based on PCR-RAPD marker. The most value of genetic distance  is 0,783 on O.c lydius male and female from hotspot at 780 masl. The percentage of polimorphic  is 71,95%. Base on similarity value and polymorphic percentage show genetic variability on intraspecies level of the Ornithoptera croesus lydius.    
Phylogenetik Study of Papilio Spp Butterfly Based on ND5 Genetik Sequence in In Silico Abdu Mas'ud; Abubakar Abdullah; Chumidach Roini
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 10, No 2 (2018): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.09 KB)

Abstract

Papilio spp is one of the members Papilionidae family. The specific character is macro lepidoptera butterflies and has beautiful colour.  The distribution of butterflies is found in Indo_Australia. Papilio spp can found on Northen Maluku and Sula archipelago region. The objectif of the research is to know genetik relationship of Papilio spp based on moleculer marker of the ND5 gene mitokondrial using insilico analysis. The genetik relationship can be knowed from the phylogenetik tree construction by sequence ND5 gene mitocondial. The Sequence ND5 gene is obstained from the National Center for Biotechnology Information (NCBI) and alignment sequence for the construction phylogenetik tree using Clustal W program acces from the National Center for Biotechnology Information (NCBI) online. The phylogenetik tree showed that Papilio ulyses from Obi island and Morotai island have one ancestor (monophyletic) with Papilio ulyses telegous from Timika.
Tinjauan Filogenetik Kupu-Kupu Ornithoptera spp. Berdasarkan Sekuen Mitokondria ND5 Gen Abdu Masud; Abubakar Abdullah
TECHNO: JURNAL PENELITIAN Vol 6, No 02 (2017): Techno Jurnal Penelitian
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tk.v6i02.568

Abstract

AbstrakOrnithoptera spp adalah salah satu kupu-kupu berukuran besar (makro lepidoptera) yang memiliki nilai estetika yang tinggi. Penyebaran kupu-kupu ini ditemukan di wilayah Maluku dan Papua. Salah satu aspek konservasi dari eksistensi kupu-kupu ini adalah database keanekaragaman dan kekerabatan. Oleh karena itu, tujuan dari studi kekerabatan  Ornitoptera spp adalah untuk mengetahui hubungan kekerabatan Ornitoptera spp berdasarkan penanda molekuler gen  ND5 pada mitokondria. Hubungan kekerabatan tersebut dapat dilihat dari pohon filogeni yang dikonstruksi berdasarkan sequence nukleotida pada  gen ND5. Sequence gen ND5 diperoleh dari National Center for Biotechnology Information (NCBI) dan alignment sequence untuk konstruksi pohon filogenetik menggunakan program Clustal W yang diakses dari National Center for Biotechnology Information (NCBI) secara online. Pohon filogenetik menunjukkan bahwa Ornithoptera alexandea asal Papua New Guinea merupakan nenek moyang dari Ornithoptera spp. Onithoptera aesachus asal Pulau Obi dan Ornithoptera croesus asal pulau Bacan merupakan spesies Ornithoptera yang paling modern dan memiliki tingkat evolusioner termuda berdasarkan pohon filogenetik.Kata kunci: Ornithoptera spp, filogenetik, ND5 gen.  AbstractOrnithoptera spp is one of macrolepidoptera butterflies, its has hight  aestehical value.    The distribution of butterflies are  found in Maluku and Papua region. One aspect as conservation of the exixtence  butterflies is the database diversity and genetic relationship. The objectif of the research is to know genetic relationship of Ornithoptera spp based on moleculer marker of the ND5 gene mitokondrial. The genetic relationship can be knowed from the phylogenetic tree construction by sequence ND5 gene mitocondial. The Sequence ND5 gene is obstained  from the National Center for Biotechnology Information (NCBI) and alignment sequence for the construction phylogenetic tree using Clustal W program acces from the National Center for Biotechnology Information (NCBI) online. The phylogenetic tree showed that Ornithoptera alexandea from Papua New Guinea is the ancestor for Ornithoptera spp. Onithoptera aesachus from Obi island dan Ornithoptera croesus from Bacan island is  Ornithoptera’s most modern spesies and has the youngest evolutionary level by phylogenetic tree.Keywords: Ornithoptera spp, phylogenetic, ND5 genes.
Co-Authors Abdu Mas'ud Chumidach Roini Masud, Abdu