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All Journal Asian Journal of Health and Applied Sciences
Wahid, Aziz Ansori
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Comparison of hemoglobin level measurement results using sodium lauryl sulphate with Oshiro and Mansoor procedure Fadhilah, Wasiyah Khusna; Al Badri, Muhammad Hilal; Wahid, Aziz Ansori; Fadhila, Feldha
Asian Journal of Health and Applied Sciences Vol. 1 No. 1 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.919 KB) | DOI: 10.53402/ajhas.v1i1.4

Abstract

The method of measuring Hb levels recommended by ICSH uses the HiCN method. However, it has a high risk due to the toxicity of the reagent. Therefore, the determination of Hb has been developed using the SLS method. According to Oshiro and Mansoor, who both stated that there was no significant difference between SLS and HiCN, this method has two distinct procedures. This study aims to determine the comparison between the measurement results of hemoglobin levels using sodium lauryl sulfate with the Oshiro procedure and Mansoor. The research design used is analytical research. The data obtained from the results of measuring Hb levels with the Oshiro and Manshoor procedures was given to students of the Diploma IV Study Program of Medical Laboratory Technology, Rajawali Health Institute batch 2018, which collected 49 people. The sampling technique used was saturated sampling to reduce the error rate in the study. The results of the normality test of the data in this study showed n of the two procedures was 49 mean±SD for the Oshiro procedure 13.09±0.56 and p = 0.200. Meanwhile, the Mansoor procedure had a meanSD of 13.09±0.57 and a p-value of 0.059. Because the probability of both being p > 0.05, the data is declared normally distributed. The results of the average difference test from the data of this study show the mean±SD of the pairwise difference between the Oshiro and Mansoor procedures is 0.002±0.059 and the p value = 0.811. If P > 0.05, then it is stated that there is no significant difference between the two groups. The conclusion of this study is that the result of measuring Hb levels using the SLS procedure is reliable. Oshiro and Mansoor conform to HiCN and there is no significant difference in the mean results.
The Effect of Different Extraction Temperatures of SARS-COV2 RT-PCR Examination Process On CT Value In Patient Specimens In Labkesda Kabupaten Sukabumi, Indonesia Fadhilah, Fitri Rahmi; Wahid, Aziz Ansori; Rachman, Mochamad Irwan Fitriana
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.39

Abstract

The Severe Acute Respiratory Syndrome Coronavirus-2, or COVID, was announced by WHO on February 11, 2020.  Examination of SARS-CoV2 by RTq-PCR method in Labkesda Kab. Sukabumi is one of the health service support units in the Sukabumi area. The number of PCR examination requests is increasing every day, whereas, at this stage, the temperature increase process should reach the recommended temperature of 90°C, which can take 20-25 minutes. Sometimes ATLM has processed specimens at 60°C - 90°C. Given the required temperature at the time of extraction, which is at a temperature of 90 degrees, here the researchers wanted to prove whether below 90 degrees had an effect on the virus extraction process. This study aims to determine the effect of differences in extraction temperature on the SARS-CoV2 RTq-PCR examination process on CT VALUE on patient specimens. The variable in this study was the difference in temperature consisting of 60°C, 80°C, and 90°C. This research used quantitative research methods with case studies. This study is an experimental study to determine the effect of temperature differences on the CT value of the RTq - PCR examination. The population of this study were 30 patients at LABKESDA Kabupaten Sukabumi. This analysis used a one-way ANOVA test analysis with software. IBM SPSS 23.0. It may be concluded that there is no significant difference in the CT Value findings on the SARS-CoV2 RTq-PCR Examination since the study's p-value was 0.758, which is more than 0.05.
Co-Authors Al Badri, Muhammad Hilal Fadhila, Feldha Fadhilah, Wasiyah Khusna Fitri Rahmi Fadhilah, Fitri Rahmi Rachman, Mochamad Irwan Fitriana