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Potensi Jeruk Nipis (Citrus aurantifolia) dalam Memutihkan Email Gigi yang Mengalami Diskolorasi Lime (Citrus aurantifolia) Potential to The Whiten Discoloration Tooth Enamel Rochmah, Nurbaetty; Merry Ch.R, Dwi; Lestari, Sri
Insisiva Dental Journal Vol 3, No 1 (2014)
Publisher : Insisiva Dental Journal

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Latar Belakang: Perubahan warna gigi (diskolorasi) merupakan problem estetika yang berdampak padapsikologis terutama pada gigi anterior. Seseorang akan melakukan perawatan terhadap giginya untuk mengatasimasalah estetiknya. Salah satu upaya dokter gigi untuk mengatasi masalah tersebut adalah bleaching. Tujuan:Mengetahui pengaruh air perasan jeruk nipis konsentrasi 2,5% terhadap email gigi yang mengalami diskolorasi,serta untuk menentukan waktu optimum air perasan jeruk nipis konsentrasi 2,5% dalam memutihkan email gigiyang yang mengalami diskolorasi dengan variasi waktu 30 menit, 45 menit dan 60 menit. Metode Penelitian:Jenis penelitian yang dilakukan adalah eksperimental laboratorik dengan rancangan pretest-posttest group. Alatuji interpretasi warna yang digunakan adalah Spectrophotometer UV-VIS 2401 PC. Analisis data menggunakanuji Paired-T test dan Oneway Annova. Hasil: Nilai uji intensitas warna sesudah perendaman air perasan jeruknipis konsentrasi 2,5% lebih tinggi dibandingkan dengan sebelum perendaman air perasan jeruk nipis konsentrasi2,5% dan tidak ada perbedaan signifikan antar kelompok (30 menit, 45 menit dan 60 menit). Kesimpulan: Jeruknipis mampu merubah warna gigi yang terdiskolorasi menjadi lebih putih dengan konsentrasi 2,5% dengan lamaperendaman 30 menit, 45 menit dan 60 menit.

Respon Limfosit T Sitotoksik Pada Gingivitis Setelah Pemberian Kurkumin (Citotoxic T Lymphocytes Response in Gingivitis After Curcumin Given) Nitawati Meilisa Ni Putu; Dwi Merry Christmarini Robin; Mei Syafriadi
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Gingivitis is a form of periodontal disease most common in the community. Gingivitis is caused by bacteria in subgingival plaque, one of them is Porphyromonas gingivalis. Curcumin is a phenolic yellow pigment obtained from the powdered rhizome of Curcuma sp. which has anti-inflammatory effects. Curcumin can reduce the number and responses of cytotoxic T lymphocyte by signing on IL-1, IL-2, IL-12, and TNF. Variables observed in this study is the number and response of cytotoxic T lymphocyte in male Wistar rats gingiva. 16 male Wistar rats were divided into two groups: control and treatment groups. To cause gingivitis rats were injected with bacteria P. gingivalis 3x108 CFU concentrations as much as 0.02 ml of the gingival sulcus maxillary left for 2 days. The control group was given PBS and treatment group was given curcumin dose of 0.03 mg / g BW in intragastric starting from day 2 after induction of P. gingivalis. Rats sacrificed on day 3 (72 hours after the induction of P. gingivalis) and day 5, followed by extraction, fixation, tissue processing to be preparations with HE staining and IHC. Observation of the results carried out using a light microscope. Results showed that there was no cytotoxic T lymphocytes in the control and treatment groups, but in HE staining found existence of lymphocytes guess is that memory T lymphocytes (CD45). The conclusion of this study is in IHC staining method there is no difference in the number of cytotoxic T lymphocytes in the control group and the treatment, but in HE staining found existence of lymphocytes guess is that memory T lymphocytes, and the clinical features are redness and swelling until day 3 which the gingival more redness in the control group compared to the treatment group and healing has occurred on day 4 to day 5 in the control and treatment groups. Keywords: Citotoxic T lymphocytes, Curcumin, Gingivitis, Immunohistochemica

Potensi Jeruk Nipis (Citrus aurantifolia) dalam Memutihkan Email Gigi yang Mengalami Diskolorasi Lime (Citrus aurantifolia) Potential to The Whiten Discoloration Tooth Enamel Nurbaetty Rochmah; Dwi Merry Ch.R; Sri Lestari
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 3, No 1 (2014)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v3i1.1731

Latar Belakang: Perubahan warna gigi (diskolorasi) merupakan problem estetika yang berdampak padapsikologis terutama pada gigi anterior. Seseorang akan melakukan perawatan terhadap giginya untuk mengatasimasalah estetiknya. Salah satu upaya dokter gigi untuk mengatasi masalah tersebut adalah bleaching. Tujuan:Mengetahui pengaruh air perasan jeruk nipis konsentrasi 2,5% terhadap email gigi yang mengalami diskolorasi,serta untuk menentukan waktu optimum air perasan jeruk nipis konsentrasi 2,5% dalam memutihkan email gigiyang yang mengalami diskolorasi dengan variasi waktu 30 menit, 45 menit dan 60 menit. Metode Penelitian:Jenis penelitian yang dilakukan adalah eksperimental laboratorik dengan rancangan pretest-posttest group. Alatuji interpretasi warna yang digunakan adalah Spectrophotometer UV-VIS 2401 PC. Analisis data menggunakanuji Paired-T test dan Oneway Annova. Hasil: Nilai uji intensitas warna sesudah perendaman air perasan jeruknipis konsentrasi 2,5% lebih tinggi dibandingkan dengan sebelum perendaman air perasan jeruk nipis konsentrasi2,5% dan tidak ada perbedaan signifikan antar kelompok (30 menit, 45 menit dan 60 menit). Kesimpulan: Jeruknipis mampu merubah warna gigi yang terdiskolorasi menjadi lebih putih dengan konsentrasi 2,5% dengan lamaperendaman 30 menit, 45 menit dan 60 menit.

ESTABLISHMENT OF A RAT MODEL OF TEMPOROMANDIBULAR JOINT OSTEOARTHRITIS USING INTRAARTICULAR INJECTION OF COMPLETE FREUND’S ADJUVANT Dwi Merry Christmarini Robin
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Osteoarthritis is a common progressive joint disease,involving not only the joint lining but also cartilage,ligaments, and bone. Osteoarthritis (OA) of thetemporomandibular joint is a unilateral,degenerative disease of the jaw joint. It ischaracterized by breakdown of the articularcartilage, architectural changes in bone, anddegeneration of the synovial tissues causing painand/or dysfunction in functional movements of thejaw. Temporomandibular joint osteoarthritis (TMJOA) is an important etiology of temporomandibularpain. The temporomandibular joint (TMJ) undergoesdegenerative changes among patients who sufferfrom arthritis, and yet the pathogenesis of TMJosteoarthritis and rheumatoid arthritis is poorlyunderstood. However, until recent years, OA of thetemporomandibular joint was confusing, relativelyunrecognized, and difficult to diagnose and manageand the specific mechanisms of TMJ OA in thedevelopment of temporomandibular pain are stillnot clear. Animal models are useful tools for theinvestigation of association between animalbehavioral and TMJ OA. Several animal models ofTMJ OA have been established using intraarticularinjection of various chemicals. This study aimed toestablish a rat model of TMJ OA using completeFreund’s adjuvant (CFA). Histopathological changewith hematoxylin-eosin staining and radiographicchange of the TMJ was performed. Treatment withCFA induced cartilage damage, synovitis, andproliferation of synovial cells in the fact joints. A ratmodel of TMJ OA was successfully established usingintraarticular injection of CFA. This animal model isconvenient and shows good resemblance to humanOA pathology. We hypothesized that inflammationin the TMJ induces structural abnormalities, andaccordingly characterized the disc and synovium in anovel model with double injections of completeFreund’s adjuvant (CFA), using histopathologicalchange with hematoxylin-eosin staining andradiographic examinition.

Cultivation and expansion of mesenchymal stem cells from human gingival tissue and periodontal ligament in different culture media Banun Kusumawardani; Dwi Merry Christmarini Robin; Endah Puspitasari; Irma Josefina Savitri; Dea Ajeng Pravita Suendi
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 1 (2021): March 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i1.p39-45

Background: Gingival tissue and periodontal ligament act as sources of mesenchymal stem cells (MSCs) that play a vital role in periodontal regeneration, but they both have limitations for cell availability. MSCs cultivated and expanded in various media formulations could be used as a basis for the development of cell therapy protocols. Purpose: This study aimed to determine the optimum culture media formulation for cultivation and expansion of human gingival-derived mesenchymal stem cells (hGMSCs) and human periodontal ligament stem cells (hPDLSCs). Methods: The hGMSCs and hPDLSCs were obtained from gingival tissue and periodontal ligament specimens from an adult patient. The two different culture media formulations used were: 1) α-minimum essential media (α-MEM) supplemented with 10% FBS, 100 U/mL penicillin, 100mg/mL streptomycin and 2.5 µg/mL amphotericin B; and 2) Dulbecco’s minimum essential media-Low Glucose (DMEM-LG) supplemented with 10% FBS, 2 mMol/L L-glutamine, 100 U/mL penicillin, 100mg/mL streptomycin and 2.5 µg/mL amphotericin B. The minced-gingival tissue and periodontal ligament samples were seeded in 3 cm tissue culture dishes with one of two experimental culture media, and incubated at 37oC in a humidified atmosphere of 5% CO2. Results: Cell morphology was observed on days two and five of the third passage. The gingival tissue and periodontal ligament primary cells exhibited fibroblast-like morphology, long processes and were spindle-shaped. The hPDLSCs grown in α-MEM exhibited a significant increase in cell viability and proliferation rate compared to the hPDLSCs grown in DMEM-LG. However, hGMSCs displayed similar cell viability and proliferation rate on both types of experimental media. Both the hGMSCs and hPDLSCs expressed MSC markers, including CD105, CD146, and CD90, but did not express CD45. Conclusion: Culture media formulations of α-MEM and DMEM-LG can be used for the cultivation and expansion of both hGMSCs and hPDLSCs.

Correlation between general health, nutrition, and oral health status of elderly in a typical retired community-house Banun Kusumawardani; Agustin Wulan Suci Dharmayanti; Dwi Merry Christmarini Robin; Yani Corvianindya Rahayu; Nurul Isnaini Fitriyana
Padjadjaran Journal of Dentistry Vol 33, No 2 (2021): July 2021
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no2.18950

Introduction: Indonesia is in an aging population era marked by an increase in elderly population above 7%. An enhancement of elderly population presents health problems due to organ function deterioration and requires extraordinary attention. General health, nutrition, and oral health status are the most problems claimed by elderly, particularly retired. Objective of this study was to analyze the correlation between the general health, nutrition, and oral health status of elderly. Methods: Analytical descriptive research with cross sectional method was carried out on 36 respondents in Paguyuban Purnabakti, Patrang, Jember, East Java Province. The sampling was done by purposive sampling. Information about social-demography and general health status of respondents were obtained from the questionnaire. The social-demography investigation comprised sex, age, education level and status of residence. While general health observation, it comprised smoking status, general check-up, presence of systemic diseases, blood pressure and blood biochemical parameters.[CS1] Nutritional status was examined by body mass index (BMI). Oral examination for identifying oral health status included the number of the teeth remaining in the oral cavity, use of dentures, oral hygiene index (OHI-s), and periodontal tissue health (CPITN). Results: The result exhibited that most of the respondents presented good general health status. However, there was no significant correlation between routine general health observation and age (p=0.906). Moreover, there was no significant relationship between blood biochemical profile (blood glucose, total cholesterol, and blood pressure level) with nutrition status (p=..., p=..., and p=... respectively), and oral health status (p=...), except with oral hygiene (p=...). Conclusion: This study is limited to Paguyuban Purnabakti, Patrang, Jember, East Java Province. Although this study could not be generated in all communities, this study concluded no relationship between general health (smoking, systemic disorders, and routine general health observation), nutritional status, and oral health in the elderly. [CS1]Disesuaikan dengan table 2

Pelatihan Perilaku Hidup Sehat Pada Siswa Sekolah Luar Biasa Negeri Jember Banun Kusumawardani; Dwi Merry Christmarini Robin; Yani Corvianindya Rahayu; Parawita Dewanti; Siti Komariyah
Abdimas Universal Vol. 4 No. 2 (2022): Oktober
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Balikpapan (LPPM UNIBA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36277/abdimasuniversal.v4i2.233

Healthy living behavior training is an effort to empower students of SLB Negeri Jember. The implementation of the training has been designed according to the abilities of the students. This activity has implemented the concept of improving life skills so that students are prevented from various diseases and are able to live healthy independently. The implementation method is as follows: 1) counseling and training on healthy living behavior and brushing teeth properly; 2) general health and oral health examinations; and 3) revitalization of School Health Unit through training of Student Health Cadres. Activities are equipped with modules and health examination record books. Activity evaluation procedures include: 1) re-examination of general health status; 2) re-examination of oral health status; and 3) supervision one month after program implementation. At the end of the activity, the students are able to wash their hands and brush their teeth properly and independently. Student Health Cadres are able to take measurements of height, weight, and body temperature. The general health profile of students is quite good, but their oral health is still poor and requires treatment from health workers.

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