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All Journal Berkala Ilmiah Biologi
Ardaning Nuriliani
Fakultas Biologi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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The Potency of Olive Oil, Coconut Oil, and Bleached Palm Oil as Clearing Agent in Histological Preparation Ruth Liananda Citra Dolok Saribu; Laili Mufli Zusrina; Murtiadi Erlan Supraitno; Ardaning Nuriliani; Bambang Retnoaji; Hendry T.S.S.G. Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6528

Abstract

Clearing is an important process in histological preparations. The aim of the clearing process is to remove alcohol from the tissues upon dehydration. The most commonly used clearing agent is xylol, however it is known that there are negative effects from using xylol. This literature study purpose to study the potential of three vegetable oils, i.e., olive oil, coconut oil, and refined palm oil, as clearing agents to replace xylol. This literature study was conducted using search engines such as Semantic Scholar, NCBI, ICAMS, IJPRSE, JOMFP, PubMed, and ResearchGate with the keywords olive oil, coconut oil, palm oil, xylol, clearing agent, and histological preparations. There is no year limitation of references in this literature study. Based on the literatures, it is known that the use of olive oil as a clearing agent produces similar histological preparation qualities to xylol. This can be seen from several criteria that were measured, including the level of tissue translucency and the ability to maintain the quality of the staining. Similar results were shown by purified coconut oil and palm oil, both of which can produce tissues with optimal levels of transparency. The conclusion from this literature study is that refined olive oil, coconut oil, and palm oil can be used as clearing agents for xylol substitutes that are safer for researchers and the environment.
Fixative Solution for Macromolecules in Histological Preparations Hayu Swari Alimi; Ria Vena Septhay Sari; Tia Apriliyani; Ardaning Nuriliani; Bambang Retnoaji; Hendry Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.6531

Abstract

The fixation process is subjected to preserve cells, tissues, and their components so that the structure can be maintained as their alive condition and prevent autolysis. This literature review purpose to determine the appropriate use of fixative solutions for several macromolecules, such as proteins, lipids, carbohydrates, and nucleic acids. The literature review data is obtained form the secondary data included books, journals, or articles which discussed about fixative solutions without a limitation on the year of publication. The databases were sourced from Google Scholar, PubMed, NCBI, Science Direct, SpringerLink, Research Gate, and Nature. The keywords for the book search were tissue processing, pathology, and histology. Keywords for searching journals or articles were protein fixation, formaldehyde fixation lipids, fixatives for nucleic acids, Glyo-Fixx fixatives, mercury chloride fixative lipids, formalin-fixed carbohydrates, and ethanol or methanol for fixative lipids. Based on the literature review conducted, it can be concluded that the use of fixative solutions is according to the macromolecules to be observed.
Co-Authors Bambang Retnoaji Hayu Swari Alimi Hendry Saragih Hendry T.S.S.G. Saragih Laili Mufli Zusrina Murtiadi Erlan Supraitno Ria Vena Septhay Sari Ruth Liananda Citra Dolok Saribu Tia Apriliyani Zuliyati Rohmah