Anny Sartika Daulay
Department of Pharmacy, Faculty of Pharmacy, Universitas Muslim Nusantara Al Washliyah

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Determination of Total Alkaloid Content of Raru (Cotylelobium lanceolatum Craib) Bark Extract Using UV-ViS Spectrophotometry Method cici andriani; Anny Sartika Daulay; Ridwanto Ridwanto; Haris Munandar Nasution
Indonesian Journal of Science and Pharmacy Vol. 1 No. 1 (2023): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

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Abstract

The raru plant (Cotylelobium  lanceolatum Craib) comes from the Dipterocarpaceae family. Raru bark is usually added to the wine to enhance the taste of the wine. raru bark has antidiabetic compounds as a lowering of sugar levels in the blood. The purpose of this study was to determine the alkaloids in the Raru plant (Cotylelobium   lanceolatum  Craib) and to determine the total alkaloid content found in the Raru plant (Cotylelobium  lanceolatum  Craib). The extraction method used is the maceration extraction method using methanol solvent with various concentrations which is then evaporated with a rotary evaporator. Whereas the quantitative test was carried out by means of the viscous extract obtained by adding 2 mL of Phosphate buffer with a pH of 4.7, 2 mL of bromocresol green (BCG), and measured at a wavelength of 272.705 nm. The yield of the extract from a concentration of 95% was 23.0779%, a concentration of 70% was 21.2144%, a concentration of 50% was 20.3274%. The correlation equation obtained from the caffeine curve is y = 0.0508X + 0.008 and the value of r = 0.999, and the levels of alkaloids with different extract concentrations. Actual alkaloid content in raru bark methanol extract 95% was 14.9094 ± 0.3697 mg/g, actual alkaloid content in raru bark methanol extract 70% was 10.0472 ± 0.4594 mg/g, actual alkaloid content in extract 50% raru bark methanol was 6.6260 ± 0.803.
Analysis of Sodium Benzoate Content in the Market Soft Beverages Using Uv-Vis Spectrophotometry Method Windi Hari Akbar; Ainil Fithri Pulungan; Ridwanto Ridwanto; Anny Sartika Daulay
Indonesian Journal of Science and Pharmacy Vol. 1 No. 1 (2023): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

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Sodium benzoate is one of the preservatives that are permitted to be used in food and beverages. Research has been carried out with the aim of knowing that carbonated drinks contain the preservative sodium benzoate, knowing the amount of sodium benzoate in soft drinks and to find out the levels of sodium benzoate in soft drinks fulfilling the maximum usage requirements according to BPOM RI Head Regulation No. 36 of 2013. This study used the uv-vis spectrophotometry method. To determine the preservative content of sodium benzoate, a qualitative test was first carried out on the sample using the color reaction method and esterification test. To determine the levels using a UV-Vis spectrophotometer at a wavelength of 269 nm with ethanol p.a as a solvent. The results of the qualitative test using the color reaction method showed that the three samples of soft drinks positively contained the preservative sodium benzoate. To validate this, an esterification test was carried out on the three samples and they produced the smell of Ambon bananas which proved that the three samples positively contained sodium benzoate. For the quantitative test using the Uv-Vis spectrophotometry method at a wavelength of 269 nm using ethanol p.a as a solvent. The results of the analysis showed that the preservative content of sodium benzoate which was calculated for each sample was for a soft drink sample a: 244.93 ± 19.9349 mg/kg. Soft drinks b: 65.3 ± 0 mg/kg, soft drinks. Soft drinks c: 78.6 ± 13.1291 mg/kg. Based on these results, it can be concluded that the levels of sodium benzoate in samples A, B, and C met the standard requirements for maximum usage limits, namely not more than 600 mg/kg.