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Pramiastuti, Oktariani
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Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Journal : Medical Laboratory Analysis and Sciences Journal

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Antibacterial activity of combined extracts of red ginger (Zingiber officinale var. Rubrum) and betel leaf (Piper betle L.) against Staphylococcus aureus and Escherichia coli Oktariani Pramiastuti; Joharoh
Medical Laboratory Analysis and Sciences Journal Vol 2 No 1 (2020): May 2020
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v2i1.45

Abstract

The use of traditional medicine can be an alternative treatment with increasing antibiotic resistance. Red ginger (Zingiber officinale var. Rubrum) and betel leaf (Piper betel L.) are plants that can be used as an antibacterial. This study aimed to determine the antibacterial activity of combined extracts of red ginger and betel leaf. This experiment was carried out by making extracts of red ginger and betel leaf with maceration and tested the antibacterial activity. The antibacterial activity test used the paper disc diffusion method. The study was conducted with five different concentrations of extract 20%, 40%, 60%, 80%, and 100%. The results showed that the combination of red ginger and betel leaf extract at a concentration of 80% had the highest inhibitory area against Staphylococcus aureus (10.6 mm) and Escherichia coli (7.5 mm). The data obtained were statistically tested using One Way Anova with a significance level of > 0.05. The results of One Way ANOVA analysis of the combination of red ginger and betel leaf extracts on Staphylococcus aureus had a significant value of 0.083, while those extracts on Escherichia coli had a significant value of 0.690. It can be concluded that the combination of red ginger and betel leaf extract at a concentration of 80% had a strong inhibitory level against Staphylococcus aureus and a moderate inhibitory level against Escherichia coli.
Co-Authors Afina Nurfauziah Aprilia Suryani Bayu Aji Prastiyo Desi Sri Rejeki Devi Ika Kurnianingtyas Solikhati Devi Ika Kurnianingtyas Solikhati Dinar Anggia Zen Dinar Anggia Zen Girly Risma Firsty Inul Maghfiroh Istriningsih, Endang Joharoh Larasati Larasati Listina, Osie Neni Agusetianti Rima Harsa Atqiya Alquraisi Siti Lailatul Karimah Venny Febriani