Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

p-Index From 2019 - 2024

P-Index

This Author published in this journals

All Journal BERITA BIOLOGI

Sukamto, L Agus

Research Center for Biology-Indonesian Institute of Sciences

Author-ID : 717843

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 4 Documents Claim Missing Document

Claim Missing Document

Articles

PERBANYAKAN TANAMAN WIEP {Grevillea papuana) SECARA KULTUR JARINGAN [Tissue Culture of Wiep Plant {Grevilleapapuana)] Sukamto, L Agus
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The population of wiep (Grevillea papuana) originated from Papua, decreased because of over cutting or over exploitation for timber, ornamental or medicine. Experiment was carried out to propagate wiep by using tissue culture method.Hypocotyls,cotyledons and nodes from seedlings grown in vitro, were used as explants. These explants were grown on Murashige & Skoog medium which macro and micronutrients were half strength, with or without hormones (BA 1 mg/1, BA 1 mg/1 + NAA 0,5 mg/1, BA2 mg/1 + NAA 0,5 mg/1, BA 1 mg/1 + 2,4-D 0,5 mg/1, and BA 2 mg/1 + 2,4-D 0,5 mg/1). All explants did not produce callus on medium without hormones, but produced callus with hormones except BA treatment on cotyledon explants. Bigger callus were produced by explants that were treated by combination BA with NAA or 2,4-D compare to those treated by BA only. Nodes and hypocotyls could produce shoots, but cotyledons did not. Three kinds of explants did not produce any roots. Node is the best explants for producing shoots. Combination of BA 1 mg/1 and 2,4-D 0,5 mg/1 caused synergistic effect to stimulate shoot production of wiep hypocotyls explants.

PARTENOKARPI: BUAH TANPABIJI - APA, MENGAPADAN BAGAIMANA Sukamto, L Agus
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fruit growth and development occurred in plant for producing the offspring. Pollination and fertilization will form embryo and seed, which produce and supply plant growth regulators (PGR) for fruit growing. The role of seed can be exchanged with supply of POR exogenously and produce seedless fruit. Seedless fruit could be produced from triploid plant by crossing between tetraploid x diploid plants, in vitro culture of endosperm/irradiated pollen and genetic engineering or treated diploid plant by using PGR, pollen stress, chemical agent/ antibiotic and environment/ plant control. The benefit of triploid plant is quicker growth and produce of seedless fruits directly, whereas diploid plant could produce seedless fruits after fruit induction with special treatment. The other advantages of seedless fruit on certain plants are increased yield, fruit size/ weight,carotene and total sugar contents, decreased harvest period, yield fluctuation and blossom-end rot. Seedless fruit is preferential of consumer and give a higher price than the seeded fruit. Recently, seedless fruits of tomato, grape, citrus, cucumber and watermelon have been produced in commercial industries

PENGARUH RADIASI DAN LOKASI TUMBUH TERHADAP PERTUMBUHAN DAN PENYAKIT HAWAR DAUN TALAS kv KETAN Sukamto, L Agus; Saefudin, Saefudin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Taro (Colocasia esculenta (L.) Schott) is an important tuber crop, grown widely in humid tropics and a source of carbohydrate for many people in Asia, Africa, and the Pacific islands. The leaves and stalks of taro are used as vegetable, as they contain a high protein that is useful for people diet in developing countries. Taro is underutilized plant that has potency as an alternative food.Plant propagation of taro is usually done by using its rhizome or stolon so that the genetic diversity is very limited. In vitro and irradiated techniques can increase its genetic diversity and multiply the plant that has superior quality and quantity.Taro "Ketan" grown tissue culture have been irradiated with gamma rays S Gy and 10 Gy were planted on two different environmental locations.There were many variants as the results of these treatments. Gamma rays and environmental locations caused genotype changes that were stolon number, plant height, corm fresh weight, and leaf blight disease.Gamma rays caused morphological changes that were orientation, undulation, outline of sinus, margin of lamina,and petiole color.

PERBANYAKAN TANAMAN WIEP {Grevillea papuana) SECARA KULTUR JARINGAN Sukamto, L Agus
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The population of wiep (Grevillea papuana) originated from Papua, decreased because of over cutting or over exploitation for timber, ornamental or medicine. Experiment was carried out to propagate wiep by using tissue culture method. Hypocotyls, cotyledons and nodes from seedlings grown in vitro, were used as explants. These explants were grown on Murashige & Skoog medium which macro and micronutrients were half strength, with or without hormones (BA 1 mg/1, BA 1 mg/1 + NAA 0,5 mg/1, BA 2 mg/1 + NAA 0,5 mg/1, BA 1 mg/1 + 2,4-D 0,5 mg/1, and BA 2 mg/1 + 2,4-D 0,5 mg/1).All explants did not produce callus on medium without hormones, but produced callus with hormones except BA treatment on cotyledon explants. Bigger callus were produced by explants that were treated by combination BA with NAA or 2,4-D compare to those treated by BA only. Nodes and hypocotyls could produce shoots, but cotyledons did not. Three kinds of explants did not produce any roots. Node is the best explants for producing shoots.Combination of BA 1 mg/1 and 2,4-D 0,5 mg/1 caused synergistic effect to stimulate shoot production of wiep hypocotyls explants.

Co-Authors Saefudin Saefudin

Title

Found 4 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 4 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 4 Documents
Search