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All Journal The Indonesian Biomedical Journal Majalah Kesehatan FKUB
Edi Handoko
Department Of Otorhinolaryngology-Head And Neck Surgery, Faculty Of Medicine, Universitas Brawijaya/Dr. Saiful Anwar General Hospital, Jl. Veteran, Malang
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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HUBUNGAN ANTARA IL-6 ADENOID DAN TONSILA PALATINA DENGAN IL-6 SERUM PADA ADENOTONSILITIS KRONIS HIPERTROFI Novita, Khuznita Dasa; Handoko, Edi; Indrasworo, Dyah
Majalah Kesehatan FKUB Vol 5, No 2 (2018): Majalah Kesehatan Fakultas Kedokteran
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.365 KB) | DOI: 10.21776/ub.majalahkesehatan.005.02.4

Abstract

 Adanya inflamasi lokal persisten pada Adenotonsilitis Kronis Hipertrofi (ATKH) menyebabkan perubahan histomorfologi berupa hiperplasi dan hipertrofi limfoid yang berhubungan dengan proliferasi sel T dan sel B. IL-6 berperan pada inflamasi kronis melalui rekrutmen monosit ke daerah inflamasi dan mengubah keseimbangan Th1/Th2 menuju dominasi Th2. Kadar IL-6 jaringan dan serum meningkat pada ATKH, namun hubungan kadar IL-6 pada adenoid dan tonsila palatina dengan kadar IL-6 serum belum diketahui. Tujuan penelitian ini adalah untuk mengetahui hubungan kadar IL-6 adenoid dan tonsila palatine dengan kadar IL-6 serum. Penelitian ini melibatkan 8 anak dengan kasus ATKH (rata-rata umur: 9,63 ± 3,23  tahun dan 8 anak kontrol sehat (rata-rata umur: 10,6 ± 3.02 tahun). Kadar IL-6 diperiksa dari serum, adenoid, dan tonsila palatina dengan metode ELISA. Data penelitian dianalisis dengan independent t-test, uji korelasi, dan regresi linear. Hasil penelitian ini kadar IL-6 serum kelompok kasus lebih tinggi secara bermakna dibandingkan dengan kontrol sehat (p < 0,05). Terdapat hubungan positif yang bermakna antara kadar IL-6 adenoid dengan serum (r = 0,881, p = 0,004). Sedangkan antara kadar IL-6 tonsila palatina dengan serum menunjukkan adanya hubungan yang tidak bermakna (r = 0,556, p = 0,197). Dapat disimpulkan bahwa kadar IL-6 pada adenoid berhubungan positip dengan kadar IL-6 serum. 
A Pilot Study on Immunohistochemical Expressions of NF-ĸB, Cyclin-D1, VEGF, and Cox-2 in Advanced Stage Laryngeal Carcinoma Pudji Rahaju; Ayunita Tri Wirattami; Ferry Sandra; Steffi Kurniawan; Khairun Nisa; Soehartono Soehartono; Edi Handoko; Ahmad Dian Wahyudiono; Hendradi Surjotomo; Hendy Setyo Yudhanto; Nanik Setjowati
The Indonesian Biomedical Journal Vol 13, No 4 (2021)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v13i4.1580

Abstract

BACKGROUND: Progression of laryngeal carcinoma can be classified with the clinical staging, however there are different patterns of progressions observed in the patient with the same clinical stage which also affects their prognoses. Therefore biomarkers should be used. Nuclear factor (NF)-ĸB, Cyclin-D1, vascular endothelial growth factor (VEGF), cyclooxygenase (Cox)-2 have been reported for laryngeal carcinoma. However, it is still unclear how these markers are expressed and correlated in advanced stage laryngeal carcinoma. Therefore current study was conducted to investigate the expressions of NF-ĸB, Cyclin-D1, VEGF and Cox-2 and their correlations in advanced stage laryngeal carcinoma.METHODS: Subjects were recruited and laryngeal biopsies were collected, fixed in formalin and prepared for immunohistochemistry. The immunohistochemistry was performed using mouse monoclonal anti-NF-kB p65, anti-Cyclin-D12 anti-VEGF, and anti-Cox-2 antibodies. The immunohistochemistry results were documented and measured using ImmunoRatio. Pearson or Spearman correlation test was used based on the results of Shapiro-Wilk test of normality. A p-value of less than 0.05 is considered statistically significant.RESULTS: Twelve male subjects were included in this study. Expressions of NF-ĸB, Cyclin-D1, VEGF dan Cox-2 were clearly observed. Mean of NF-ĸB, Cyclin-D1, VEGF dan Cox-2 IHC expression levels measured with ImmunoRatio were 57.50±20.06%, 45.00±24.31%, 43.33±17.23% and 40.42±16.98%, respectively. There was significant correlation between the expressions of VEGF dan Cox-2 (p=0.031, r=0.622).CONCLUSION: Since correlation between the VEGF and Cox-2 expressions was statistically significant, VEGF and Cox-2 might have important roles in the growth, invasion and metastasis of laryngeal carcinoma.KEYWORDS: advanced stage laryngeal carcinoma, immunohistochemistry, NF-ĸB, Cyclin-D1, VEGF, Cox-2
Co-Authors Ahmad Dian Wahyudiono Ayunita Tri Wirattami Dyah Indrasworo Ferry Sandra Hendradi Surjotomo Hendy Setyo Yudhanto Khairun Nisa Nanik Setjowati Novita, Khuznita Dasa Pudji Rahaju Soehartono Soehartono Steffi Kurniawan