Dental Journal (Majalah Kedokteran Gigi)
Vol. 52 No. 1 (2019): March 2019

The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans

I Dewa Ayu Ratna Dewanti (Faculty of Dentistry, Universitas Jember, Jember)
I Dewa Ayu Susilawati (Faculty of Dentistry, Universitas Jember, Jember)
P. Purwanto (Faculty of Dentistry, Universitas Jember, Jember)
Pujiana Endah Lestari (Faculty of Dentistry, Universitas Jember, Jember)
Roedy Budirahardjo (Faculty of Dentistry, Universitas Jember, Jember)
Dyah Setyorini (Faculty of Dentistry, Universitas Jember, Jember)
Ristya Widi Endah Yani (Faculty of Dentistry, Universitas Jember, Jember)
Erawati Wulandari (Faculty of Dentistry, Universitas Jember, Jember)
Melok Aris Wahyukundari (Faculty of Dentistry, Universitas Jember, Jember)

Article Info

Publish Date
31 Mar 2019

Abstract

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.

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Journal Info
Dental Journal (Majalah Kedokteran Gigi)
Website

Abbrev

MKG

Publisher

Universitas Airlangga

Subject

Dentistry

Description

The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly ...