Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Vol 13, No 2 (2008): June 2008

Isolasi Bakteri Mananolitik dan Karakterisasi Mananasenya

Meryandini, Anja (Unknown)
Anggreandari, Rizky (Unknown)
Rachmania, Nisa (Unknown)

Article Info

Publish Date
23 Oct 2019

Abstract

Isolate RA05 has the highest mannanolytic index and mannanase activity which isolated from copra soil waste from Pasaman, West Sumatra. The best growth condition that produces best mannanase activity of isolate RA05 was achieved from 500 ml flask containing 100 ml medium with 100 rpm agitation. Isolate RA05 showed its mannanase activity in medium containing Locust Bean Gum and coconut meal but not in medium containing kolang kaling. This mannanase had the highest activity on medium containing 2% of coconut meal with optimum condition temperatur 800C and pH 2.5. Adding of 5 mM MnCl2 on the crude enzym increased the activity near 300%. Other kation (Ca2+, Zn2+, Cu2+, Mg2+, Fe2+ dan Co2+) did not display great effect on the activity.

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Journal Info
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Website

Abbrev

biota

Publisher

Universitas Atma Jaya Yogyakarta

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN ...