Journal of Agricultural Science and Biotechnology
Vol 5 No 1 (2016)

DETEKSI SIMULTAN CMV DAN CHIVMV PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI DENGAN DUPLEX RT-PCR

I Gede Agus Adi Chandra (Program Magister Bioteknologi Pertanian, Program Pascasarjana Universitas Udayana)
I Dewa Nyoman Nyana (Laboratorium Penyakit Tumbuhan, Fakultas Pertanian, Universitas Udayana)
I G N Alit Susanta Wirya (Laboratorium Penyakit Tumbuhan, Fakultas Pertanian, Universitas Udayana)
Gede Suastika (Laboratorium Virologi Tumbuhan, Institut Pertanian Bogor)

Article Info

Publish Date
11 Jul 2016

Abstract

Detect the presence virus causes mosaic symptoms by CMV and ChiVMV infecting chili pepper plants at the same time was conducted. There needs a study that aims to detect the associations of both viruses causes mosaic symptoms by single or double infection and simplify the detection process which is necessary for a long and expensive process. The used method is a duplex RT-PCR in hopes of simultaneously detecting two viruses causes mosaic disease. Based on the results of the study showed that the duplex RT-PCR method can detect double infection of CMV and ChiVMV on chili plants and this method more effective to detect two viruses simultaneously in a single PCR reaction.

Copyrights © 2016




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Journal Info
Journal of Agricultural Science and Biotechnology
Website

Abbrev

JASB

Publisher

Universitas Udayana

Subject

Agriculture, Biological Sciences & Forestry

Description

Mempublikasikan hasil-hasil penelitian yang berbasis bioteknologi dalam pemanfaatannya dibidang pertanian, termasuk biological control, pascapanen, serta semua penelitian tentang pemanfaatan komponen biologi yang dikemas dalam teknologi dalam lingkup pertanian secara ...