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Gede Suastika
Laboratorium Virologi Tumbuhan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry

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PENGENDALIAN PENYAKIT VIRUS PADA TANAMAN CABAI RAWIT (CAPSICUM FRUTESCENS L.) DENG¬AN MULSA PLASTIK HITAM DAN PERAK Dewa Nyoman Darmayasa; I Gede Rai Maya Temaja; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The research of virus diseases management in chili pepper plant (Capsicum frutescens L.) by utilizing black and silver plastic mulch, was conducted in order to find the capability of black and silver plastic mulch in controlling virus diseases in chili pepper plant and also to find the capability of black and silver mulch in increasing yield of chili pepper plant. This research used randomized block design with 3 treatments and 9 replications. The result of the research shows that utilization of silver plastic mulch had ability to control virus diseases indirectly. The yield of chili pepper has incrising up to 143% with silver plastic mulch treatments.
DETEKSI SIMULTAN CMV DAN CHIVMV PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI DENGAN DUPLEX RT-PCR I Gede Agus Adi Chandra; I Dewa Nyoman Nyana; I G N Alit Susanta Wirya; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Detect the presence virus causes mosaic symptoms by CMV and ChiVMV infecting chili pepper plants at the same time was conducted. There needs a study that aims to detect the associations of both viruses causes mosaic symptoms by single or double infection and simplify the detection process which is necessary for a long and expensive process. The used method is a duplex RT-PCR in hopes of simultaneously detecting two viruses causes mosaic disease. Based on the results of the study showed that the duplex RT-PCR method can detect double infection of CMV and ChiVMV on chili plants and this method more effective to detect two viruses simultaneously in a single PCR reaction.
IDENTIFIKASI SPESIES POTYVIRUS PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI (Capsicum frutescens L.) MELALUI SIKUEN NUKLEOTIDA GEN Coat Protein I Gede Rian Pramarta; I Gede Rai Maya Temaja; I Dewa Nyoman Nyana; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Chili peppers (Capsicum frutescens L.) is one of the important vegetable in the world and one of the leading horticultural commodities in Indonesia. Pepper plants grown in all provinces in Indonesia and got priority for development because it has great potential economic value. These researches was conducted in order to determine the type of infecting Potyvirus on chili and know the proximity nucleotide sequence of Potyvirus infecting chili crop in Kerta village, Payangan, Gianyar, Bali, with the kind of Potyvirus in other areas. This study use RT-PCR technique to detect plant viruses and continued with tracing sequence coat protein gene nucleotide to its proximity to some of the isolates analyzed from GeneBank. The results showed that the infecting Potyvirus chili crop is Chili veinal motle virus species (ChiVMV) with size of approximately 900 bp DNA band, and based on phylogenetic analysis of coat protein gene nucleotide sequence, PayanganIndo isolate had high similarity (93%) and small genetic distance (0.041) with isolate of origin Thailand state.
Co-Authors Dewa Nyoman Darmayasa Gusti Ngurah Alit Susanta Wirya I Dewa Nyoman Nyana I Dewa Nyoman Nyana I G. R. M. TEMAJA I Gede Agus Adi Chandra I Gede Rian Pramarta