Jurnal Veteriner
Vol 13 No 1 (2012)

Molecular Detection of Toxoplasmosis Using Specific Primers P30, B1, and rDNA

Wisnu Nurcahyo (Unknown)
Joko Prastowo (Unknown)
Ana Sahara (Unknown)

Article Info

Publish Date
29 Oct 2012

Abstract

Study in order to develop molecular techniques using specific primers for the early diagnosis oftoxoplasmosis have been conducted. Detection of Toxoplasma gondii genome was performed usingpolymerase chain reaction (PCR) technique. The primers used in this study were rDNA, P30, and B1. ThePCR products were further run using gel electrophoresis (gel 1.5% – 2.0%) and the band was documented.Toxoplasma was detected at 500 bp and 600 bp using primer P30 and B1, respectively. Whereas usingprimer rDNA no band was observed. It was assumed that primer rDNA was not sensitive since the targetamplification was 88 bp.

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Journal Info
Jurnal Veteriner
Website

Abbrev

jvet

Publisher

Universitas Udayana

Subject

Veterinary

Description

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah ...