OLDI (Oseanologi dan Limnologi di Indonesia)
Vol 4, No 3 (2019)

KARAKTERISASI ENZIM XILANASE DARI ISOLAT BACILLUS SP. UJ131 DI HUTAN MANGROVE MARGASARI LAMPUNG TIMUR SEBAGAI KANDIDAT PROBIOTIK

Sumardi, Sumardi (Unknown)
Farisi, Salman (Unknown)
Ekowati, Christina Nugroho (Unknown)
Hairisah, Suminta Frida (Unknown)



Article Info

Publish Date
31 Dec 2019

Abstract

Characterization of Xilanase Enzyme by Bacillus sp. UJ131 A Probiotic Candidate from the Mangrove of Margasari, East Lampung. Mangrove are known to have high biodiversity as a potential location for exploration of enzyme-producing bacteria, one of which is the xylanase enzyme. Xylanase is an enzyme that hydrolyzes the xylan-rich hemicellulose substrate. This research aims to produce and know the character of xylanase enzyme by Bacillus sp. UJ131 isolate which was isolated from the oriental shrimp in Margasari mangrove forest of East Lampung. Xylanase enzymes are produced from the bacteria by growing them in sea water complete (SWC) broth containing xylan beechwood. Characterization xylanase enzyme consist of pH, temperature, and metal ions. The results showed that xylanase had an optimal time of 18 hours with xylanase activity of 0.18 U/ml, and optimally at pH 6 and temperature 70°C. The addition of Mn2+ and Ca2+ ions increase enzyme activity, but Fe3+, Cu2+ and Mg2+ ions decrease xylanase enzyme activity. At a maximum reaction speed of 0.25 U/ml, the xylanase enzyme can produce 1.30 ?M xylose per minute

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Journal Info

Abbrev

oldi

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

Description

Oseanologi dan Limnologi di Indonesia is a scientific journal that publishes original research articles and reviews about all aspects of oceanography and limnology. Manuscripts that can be submitted to Oseanologi dan Limnologi di Indonesia is the result of research in marine and inland waters in ...