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KARAKTERISASI ENZIM XILANASE DARI ISOLAT BACILLUS SP. UJ131 DI HUTAN MANGROVE MARGASARI LAMPUNG TIMUR SEBAGAI KANDIDAT PROBIOTIK Sumardi, Sumardi; Farisi, Salman; Ekowati, Christina Nugroho; Hairisah, Suminta Frida
OLDI (Oseanologi dan Limnologi di Indonesia) Vol 4, No 3 (2019)
Publisher : Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oldi.2019.v4i3.201

Abstract

Characterization of Xilanase Enzyme by Bacillus sp. UJ131 A Probiotic Candidate from the Mangrove of Margasari, East Lampung. Mangrove are known to have high biodiversity as a potential location for exploration of enzyme-producing bacteria, one of which is the xylanase enzyme. Xylanase is an enzyme that hydrolyzes the xylan-rich hemicellulose substrate. This research aims to produce and know the character of xylanase enzyme by Bacillus sp. UJ131 isolate which was isolated from the oriental shrimp in Margasari mangrove forest of East Lampung. Xylanase enzymes are produced from the bacteria by growing them in sea water complete (SWC) broth containing xylan beechwood. Characterization xylanase enzyme consist of pH, temperature, and metal ions. The results showed that xylanase had an optimal time of 18 hours with xylanase activity of 0.18 U/ml, and optimally at pH 6 and temperature 70°C. The addition of Mn2+ and Ca2+ ions increase enzyme activity, but Fe3+, Cu2+ and Mg2+ ions decrease xylanase enzyme activity. At a maximum reaction speed of 0.25 U/ml, the xylanase enzyme can produce 1.30 ?M xylose per minute
Faktor determinan financial distress pada perusahaan sektor manufaktur yang terdaftar di bursa efek indonesia Wahyuni, Sri Fitri; Farisi, Salman; Jufrizen, Jufrizen
INOVASI Vol 16, No 2 (2020)
Publisher : Faculty of Economics and Business Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.558 KB) | DOI: 10.29264/jinv.v16i2.7313

Abstract

Penelitian ini bertujuan untuk menganalisis pengaruh secara parsial dan simultan Current Ratio, Debt To Equity Ratio,  Return On Equity terhadap Financial Distress pada Perusahaan Sektor Manufaktur yang terdaftar di Bursa Efek Indonesia (BEI) periode 2013-2017. Pendekatan penelitian yang digunakan dalam penelitian inilah dengan menggunakan pendekatan asosiatif. Penelitian ini dilakukan di Bursa Efek Indonesia (BEI) khusus perusahaan sektor manufaktur yang terdaftar di Bursa Efek Indonesia (BEI) periode 2013-2017 dengan mengumpulkan data laporan keuangan yang tersedia disitus resmi www.idx.co.id. Populasi dalam penelitian ini adalah perusahaan sektor manufaktur yang terdaftar di Bursa Efek Indonesia yang berjumlah 20 perusahaan yang terdaftar di BEI periode 2013-2017.  Dan yang menjadi sampel dalam penelitiaan ini berjumlah 10 dari 20 perusahaan sektor manufaktur yang terdaftar di Bursa Efek Indonesia periode 2013-2017. Tehnik analisis data yang digunakan dalam penelitian ini adalah analisis regresi logistik. Hasil penelitian menunjukkan bahwa secara parsial Current Ratio berpengaruh signifikan terhadap Financial Distress. Secara parsial Debt To Equity Ratio dan Return On Equity tidak berpengaruh signifikan terhadap Financial Distress pada Perusahaan Sektor Manufaktur yang terdaftar di Bursa Efek Indonesia. Sedangkan, secara simultah hasil Current Ratio, Debt To Equity dan Return On Equity berpengaruh signifikan terhadap Financial Distress pada Perusahaan Sektor Manufaktur yang terdaftar di Bursa Efek Indonesia.
AKTIVITAS ENZIM HIDROLASE PADA PENAPISAN ISOLAT ACTINOMYCETES TOLERAN SALINITAS Sumardi, Sumardi; Qatrunada, Vanya; Farisi, Salman; Arifiyanto, Achmad; Ekowati, Christina Nugroho; Farisi, Salman
BIOMA Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v6i1.3548

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.
AKTIVITAS ENZIM HIDROLASE PADA PENAPISAN ISOLAT ACTINOMYCETES TOLERAN SALINITAS Sumardi, Sumardi; Qatrunada, Vanya; Farisi, Salman; Arifiyanto, Achmad; Ekowati, Christina Nugroho; Farisi, Salman
BIOMA Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v6i1.3548

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.