BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Vol. 14 No. 2 (2007)

CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE

Anja Meryandini (Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia)
Deden Saprudin (Department of Chemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia)
Prima Agung Prihandono (Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia)
Alina Akhdiya (Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD), Jl. Tentara Pelajar 3A, Bogor, Indonesia)
Trio Hendarwin (PT Sari Husada tbk, Jl. Kusumanegarano 173, Yogyakarta, Indonesia)

Article Info

Publish Date
02 Sep 2008

Abstract

Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.  Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.

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Journal Info
BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Website

Abbrev

biotropia

Publisher

Southeast Asian Regional Centre for Tropical Biology

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Description

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and ...