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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Kimia Riset Al-Kimia Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Journal of Applied Materials and Technology

Deden Saprudin

Department Of Chemistry, Faculty Of Mathematics And Natural Science, Bogor Agricultural University,Jl. Raya Darmaga, Bogor, Indonesia

Author-ID : 251010

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Engineering Immunology & microbiology Mechanical Engineering Veterinary Other

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Articles

Characterization of Xylanase Streptomyces spp. SKK1-8 ANJA MERYANDINI; TRIO HENDARWIN; DEDEN SAPRUDIN; YULIN LESTARI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 0C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 0C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 0C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan. Key words: xilanase, Streptomyces spp., characterization, zymogram and SDS-PAGE, stability

Gold Mine Wastewater Induced Morpho-physiological Alteration of Four Biodiesel Producing Species Hamim Hamim; Lutfiani Lutfiani; Deden Saprudin; Putri Nur Azizah Hutagalung; Hadisunarso Hadisunarso; Luluk Setyaningsih; Hirmas Fuady Putra
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.63

This experiment aimed to determine the adaptability of four biodiesel-producing plants to gold mine wastewater based on morpho-physiological properties. Four species namely jatropha (Jatropha curcas), castor bean (Ricinus communis), wild candlenut (Reutealis trisperma) and bead tree (Melia azedarach) were grown in water culture using Hoagland solution for 2 weeks and then treated with gold mining wastewater at 0% (control), 25%, 50%, and 75% (v/v) for 3 weeks. Growth, anatomical and physiological characteristics were observed during the treatment. The results showed that gold mine wastewater significantly decreased growth of the four species despite varied among the species. Wastewater had significant effect on leaves anatomy of Jatropha but not on others. It also significantly induced the increase of malondialdehyde (MDA) content in Jatropha and castor bean, but not in wild candlenut and bead-tree. Meanwhile, the treatment significantly decreased chlorophyll content of all species with the most in bead-tree plant, and leaves relative water content (RWC) particularly in castor bean and bead-tree plants. There was strong negative correlation between the increase of MDA content and the decrease of chlorophyll content and leaf RWC. Among the four species, wild candlenut (R. trisperma) was the most resistant to gold mine wastewater based on morphological and physiological properties.

Pengembangan Pupuk Cair Nitrogen Berukuran Nanometer untuk Meningkatkan Efisiensi Pemupukan Deden Saprudin; Munif Gulamahdi; Wiwik Hartatik; Latifah Kosim Darusman; Ilfa Nuraisyah
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 2 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.569 KB)

Conventional utilization of urea just has absorbed 30-60%. Remaining, 40-70% lost to environment, give not economic benefit and serious problem in environment. The use of less efficient urea has received attention lately. One way to overcome the problem of inefficiency of urea fertilizer use is a nano-sized fertilizer. Synthesized nanofertilizer in this study were nanomagnetit (Fe3O4). Nanomagnetit synthesized from FeCl3 as a source of iron, sodium citrate as the reductant, and urea as a source base. Nanomagnetite synthesized using a hydrothermal method. Based on the results of X-ray diffraction, the nanomagnetit has average crystal size 46.66 nm and crystal structure is Face Center Cubic. Energy-dispersive X-ray spectroscopy shows constituent elements in nanomagnetite are iron (29.45%), oxygen (53.07%), carbon (14.86%), and sodium (2.62%). This shows the iron and oxygen is the main constituent elements nanomagnetite. In the process of magnetite formation absorbed urea by 30% with N content (0.14%) and most are in the liquid synthesis (0.82%) so that it can be used as fertilizer on crops. Based on this research, variations of pH (6-8) liquid fertilizer provided no significant effect. The addition of 0.15 g nanomagnetite have better growth among other treatments.

Uric Acid Biosensor Based on Biofilm of L. plantarum using Screen-Printed Carbon Electrode Modified by Magnetite Dian Siska RF; Deden Saprudin; Dyah Iswantini; Novik Nurhidayat
Al-Kimia Vol 6 No 2 (2018): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v6i2.6300

Biosensor based on biofilm of L. plantarum has been successfully done for determination of uric acid in human urine compared with colorimetric enzymatic produced relative error of less than 5%. L. plantarum has uricase activity to react with uric acid, to maintain the stability of bacteria forming themselves into biofilms. Magnetite is known to increase sensitivity of the biosensor. The combination of magnetite-polyethylene glycol (Fe3O4-PEG) was used to modify the surface of Screen-Printed Carbon Electrode modified (SPCE) and the resulting modified electrode (biofilm/Fe3O4/PEG/SPCE) displayed good electrocatalytic activity to the oxidation of UA. The composition of biofilms with optical density 1, magnetite 100 mgmL-1 and PEG 3% v / v were able to increase the current up to 48% in 4mM of UA. The biosensor with an optimum composition produced good linearity with a concentration range, limit of detection, limit of quantitation, sensitivity, and repeatability were found to be 0.1 - 4.3 mM, 70 µM, 234 µM, 25.392 µA mM-1, 2.38%, respectively. This biosensor stable up to 49 days of measurement with the remaining activity was 90.70% and selective for interference compounds such as salt, urea, glucose, ascorbic acid. This method has a good stability, sensitivity, and potential application in clinical analysis.

CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE Anja Meryandini; Deden Saprudin; Prima Agung Prihandono; Alina Akhdiya; Trio Hendarwin
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 14 No. 2 (2007)
Publisher : SEAMEO BIOTROP

Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.Â Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.

GROWTH, PHOTOSYNTHESIS AND PROLINE ACCUMULATION OF METAL-ACCUMULATOR WEEDS Hamim Hamim; Rani Apriani; Deden Saprudin; Yohana C Sulistyaningsih
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Heavy metals especially lead and mercury contaminant have been spread widely because of their intensive utilization in industry or extraction in mining area which threaten our environment. The experiment aimed to examine the growth and some physiological parameters of five metal-accumulator weed species in response to mercury (Hg) and lead (Pb) treatment. Five weed species (Branchiaria mutica, Cyperus kyllingia, Ipomea aquatica, Mikania micrantha, and Paspalum conjugatum) were grown in water culture using half strength Hoaglandâ€™s solution and subjected to Hg(NO3)2 and PB(NO3)2 at 0, Â 0.25 and 0.5 mM for 3 weeks. The growth, photosynthesis, lipid peroxidation and proline content were observed during the treatments. The result showed that both Hg and Pb decreased growth significantly, but the decrease was far higher in Hg than in Pb treatments. Hg treatment reduced photosynthetic rate dramatically under different photosynthetic photon flux density suggesting that heavy metal Hg until 0.5 mM caused the damage of photosynthetic apparatus almost all species except in I. aquatica. Hg and Pb treatment caused dramatic increase in leaf MDA content, which was associated with the decrease of chlorophyll content significantly. Almost all the species were tolerant to Pb treatment up to 0.5 mM except M. micrantha, while only C. kyllingia and I. aquatica were tolerant to Hg treatment up to 0.5 mM. Only Hg treatment and not Pb that induced higher proline content in the leaves of threated plants without clear pattern of the increment among the species suggesting that proline may have a role as alarm stress rather than tolerant indicator.

Evaluasi Pemberian Unsur Hara Besi pada Kandungan Asam Amino dan Mineral dalam Biji Jagung Deden Saprudin; Citra Ajeng Palupi; Eti Rohaeti
Jurnal Kimia Riset Vol. 4 No. 1 (2019): Juni
Publisher : Universitas Airlangga

Evaluasi pemberian pupuk nanomagnetit (Fe3O4) 25 ppm dan pupuk komersial Fe-kelat 250 ppm terhadap kandungan nutrisi dalam biji jagung seperti kandungan proksimat, asam amino, serta mineral telah dilakukan. Asam amino ditentukan menggunakan kromatografi cair kinerja tinggi (KCKT) dan mineral ditentukan menggunakan spektrofotometri serapan atom (SSA). Pengolahan data melalui uji anova satu arah untuk membandingkan kedua jenis perlakuan. Perlakuan Fe-kelat 250 ppm dapat meningkatkan kandungan total asam amino 32% dan total mineral 86%. Begitupun perlakuan nanomagnetit 25 ppm juga terbukti meningkatkan kandungan total asam amino sebesar 18% dan total mineral 29% pada biji jagung secara signifikan jika dibandingkan dengan kontrol berdasarkan uji anova.

The Oriented Attachment Crystal Growth Model in Hydrothermal Synthesis of Magnetite (Fe3O4) Nanoparticles Ahmad Fadli; Amun Amri; Esty octiana sari; Sukoco Sukoco; Deden Saprudin
Journal of Applied Materials and Technology Vol. 1 No. 1 (2019): September 2019
Publisher : AMTS and Faculty of Engineering - Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/Jamt.1.1.15-19

The magnetite nanoparticles (Fe3O4) are very promising nanomaterials to be applied as drug delivery due to their excellent superparamagnetic, biocompatibility and easily modified surface properties. Those properties are influenced by the structure and size of the material which can be controlled by studying the evolution of crystal growth. The purpose of this research is to study the evolution of crystal growth of magnetite nanoparticles in the hydrothermal system and determine the crystal growth kinetics using the Oriented Attachment Growth model. Magnetite nanoparticles were synthesized using a hydrothermal method from FeCl3, citrate, urea and polyethylene glycol at 210?C for 1 - 12 hours at a various concentration of FeCl3 (0.05 M, 0.10 M, and 0.15 M). The characterizations were conducted by X-ray Diffraction (XRD), Transmission Electron Microscope (TEM), Particle size analyzer (PSA), and Vibrating Sample Magnetometer (VSM). The XRD difractogram indicated that the magnetite was begun to form at 3.5 hours synthesis. The crystallinity and the crystal size of magnetite rose with reaction time. The diameter of magnetite crystals was in the range of 9.4-30 nm. Characterization by TEM showed that the particles were formed from a smaller particles which were then agglomerated. The PSA characterization showed that the distribution of diameter size enlarged with the enhancement of concentrations. VSM result showed that the magnetite nanoparticle has superparamagnetic properties. The magnetite crystal growth can be fitted by the Oriented Attachment Growth model with an error of 29%.

Peningkatan kesadaran sanitasi masyarakat pedesaan melalui diseminasi pembuatan sabun transparan Obie Farobie; Deden Saprudin; Shaeful Firmansyah
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5, No 1 (2022): Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i1.13822

PRODUKSI PUPUK ORGANIK PADAT DARI LIMBAH SERABUT KELAPA SAWIT DENGAN BAHAN PENUTUP GEOTEKSTIL Fahrizal Hazra; Deden Saprudin; Mohammad Khotib; Kevin Setiawan
Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Vol 22, No 1 (2023): Maret 2023
Publisher : Universitas 17 Agustus 1945 Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31293/agrifor.v22i1.6371

Serabut kelapa sawit memiliki C/N rasio yang tinggi sehingga dalam pemanfaatannya sebagai pupuk organik padat diperlukan proses pengomposan agar dapat menurunkan C/N rasio. Bahan penutup yang baik diperlukan dalam proses pengomposan guna menghasilkan pupuk organik padat yang efektif dan memenuhi syarat mutu. Tujuan penelitian ini adalah memproduksi pupuk organik padat dari limbah serabut kelapa sawit menggunakan bahan penutup geotekstil dengan pembanding penutup plastik, serta dilakukan pengujian kualitas fisik, kimia, dan biologi. Penelitian ini menggunakan rancangan acak lengkap (RAL) 2 × 2. Faktor pertama merupakan bahan penutup (P), yang terdiri atas 2 taraf yaitu, bahan penutup geotekstil (P1) dan bahan penutup plastik (P2). Faktor kedua adalah penambahan mikrob (C), yang terdiri 2 taraf yaitu, tanpa penambahan mikrob (C0), dan dengan penambahan mikrob (C1). Proses pengomposan dilakukan selama 30 hari dan dilakukan pembalikan setiap 3 hari. Hasil penelitian menunjukkan bahwa produksi pupuk organik padat kualitas terbaik terdapat pada perlakuan dengan bahan penutup geotekstil yang menghasilkan warna coklat kehitaman dan beraroma seperti tanah, pH dan total hara makro (N+P2O5+K2O) yang telah memenuhi syarat berdasarkan Keputusan Menteri Pertanian No. 261 Tahun 2019, serta total mikrob yang sangat tinggi.

Co-Authors Ahmad Fadli Alina Akhdiya Amun Amri Anja Meryandini Anja Meryandini Citra Ajeng Palupi Dian Siska RF Dyah Iswantini Esty Octiana Sari Eti Rohaeti Fahrizal Hazra Hadisunarso Hadisunarso Hamim Hamim Hamim Hamim Hirmas Fuady Putra Ilfa Nuraisyah Kevin Setiawan Latifah Kosim Darusman Luluk Setyaningsih Lutfiani Lutfiani Mohammad Khotib Novik Nurhidayat Obie Farobie Prima Agung Prihandono Putri Nur Azizah Hutagalung Rani Apriani Shaeful Firmansyah Sukoco Sukoco Trio Hendarwin TRIO HENDARWIN Wiwik Hartatik Yohana C Sulistyaningsih YULIN LESTARI

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