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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Trio Hendarwin
PT Sari Husada tbk, Jl. Kusumanegarano 173, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE Anja Meryandini; Deden Saprudin; Prima Agung Prihandono; Alina Akhdiya; Trio Hendarwin
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 14 No. 2 (2007)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.57 KB) | DOI: 10.11598/btb.2007.14.2.16

Abstract

Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.  Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.
Co-Authors Alina Akhdiya Anja Meryandini Deden Saprudin Prima Agung Prihandono