JURNAL KIMIA SAINS DAN APLIKASI
Vol 22, No 6 (2019): Volume 22 Issue 6 Year 2019

Total Hyptolide of Indonesian Hyptis pectinata extracts in a various solvent using HPLC and UV-Vis spectroscopy and their toxicities

Meiny Suzery (Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang)
Siti Khumairoh (Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang)
Bambang Cahyono (Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH., Tembalang, Semarang)

Article Info

Publish Date
30 Nov 2019

Abstract

The bioactive compound of hyptolide has been isolated from an Indonesian plant Hyptis pectinata using various solvents. The pure compound was then used as a standard in quantitative analysis using HPLC and UV-Vis spectroscopy in ethanol (EEth), ethyl acetate (EEa), acetone (Eac) and hexane (Ehex) extract each earned 3.14%, 0.01%, 1.33% and 0.04% (dry weight per sample). The standard curve of hyptolide using UV spectroscopy has been obtained with the coefficient of relationship (r) of 0.997. However, the use of spectroscopy was not recommended for the standardization of hyptolide in the extract due to interference from other compounds that absorb the same wavelength. Furthermore, the toxicity test using the Brine Shrimp lethality test shows LC50 value of 92-181 ppm, which was in EEa compared to EEth, Eac, and Ehex.

Copyrights © 2019




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Full PDF
2252.298 KB
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
JURNAL KIMIA SAINS DAN APLIKASI
Website

Abbrev

ksa

Publisher

Universitas Diponegoro

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

Description

urnal Kimia Sains dan Aplikasi (p-ISSN: 1410-8917) and e-ISSN: 2597-9914) is published by Department of Chemistry, Diponegoro University. This journal is published four times per year and publishes research, review and short communication in field of ...