SIMBIOSIS
Vol 8 No 1 (2020)

BIODEGRADATION OF CACAO (Theobroma cacao L.) RIND WASTE BY Aspergillus niger WITH VARIATION OF INOCULUM CONCENTRATION AND INCUBATION TIME

Alfian Siswanto (student)
Ida Bagus Gede Darmayasa (Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana)
I Ketut Muksin (Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana)



Article Info

Publish Date
08 Apr 2020

Abstract

Crop waste or cacao (Theobroma cacao L.) processing waste that doesn’t handle with care can polluting the environment around the farm. Rind of cacao can be used for composting, but it can be less effective because the nutrient of fresh cacao rind too complex and the ratio of C/N too high. This research intention to knows the potential of Aspergillus niger to degrade cellulose on waste cacao rind who have high ratio of C/N, with combination inoculum concentration and incubation time. The methods of this research begin with isolation and identification of A. niger from litter of cacao field, then the in vitro test done by CMCA (Carboxyl Methyl Cellulose Agar) selective media, and then the cacao rind was testing by in vivo method. The results of this research shown that A. niger can degrade cellulose by in vitro ways, it can be seen by the clear zone that formed on CMCA selective media. The in vivo test on cacao rind shown decreasing of C/N ratio between 18,940%-40,623%. Combination of A3B3 treatment (15 mL inoculum concentration, 28 days incubation time) indicate the lowest C/N ratio 18,940%.

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