journal of Basic Medical Veterinary
Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020

PENETAPAN RETENTION TIME PROGESTERON DALAM PELARUT ELUEN PHASE MOBILE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Evania Haris Chandra (Fakultas Kedokteran Hewan Universitas Airlangga)
Mochamad Lazuardi (Fakultas Kedokteran Hewan Universitas Airlangga)
Suryanie Sarudji (Fakultas Kedokteran Hewan Universitas Airlangga)
Lilik Maslachah (Fakultas Kedokteran Hewan Universitas Airlangga)
Mohammad Sukmanadi (Fakultas Kedokteran Hewan Universitas Airlangga)
Suzanita Utama (Fakultas Kedokteran Hewan Universitas Airlangga)



Article Info

Publish Date
01 Aug 2020

Abstract

The aim of this research was to determine the level of stability of Progesterone-retention time on Eluen Phase Mobile (EPM) solution in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The results showed that the progesterone treatment in EPM solvents with six-hour storage (P1) and eight hours (P2) retention time progesterone are stable but the progesterone treatment in EPM solvent with 12-hour storage (P3) showed an unstable due to storage time. Besides that there are irrelevant peak which show that many pure molecular fractions are separated in the stationary phase so that the molecular structure of the substance begins to break down. Based on these results, it can be concluded that the storage time of Progesterone in Eluen Phase Mobile began to be unstable at 12 hours of storage. 

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Journal Info

Abbrev

JBMV

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, ...