Buletin Peternakan
Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020

Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

Rony Marsyal Kunda (Department of Biology, Faculty of Mathematics and Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia)
Slamet Diah Volkandari (Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI) Bogor, 16911, Indonesia)
Maman Rumanta (Department of Biology, Faculty of Teacher Training and Education, Universitas Terbuka, Tangerang Selatan, 15418, Indonesia)
Pieter Kakisina (1Department of Biology, Faculty of Mathematics and Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia)



Article Info

Publish Date
30 Nov 2020

Abstract

Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using HaeIII enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequilibrium genetic. It maybe caused limited buck and nonrandom mating in population that effect of low genetic variation. Inbreeding study are needed to explore it. The introgression of bucks from other families in several locations within Lakor island can be an alternative solution to increase the genetic diversity of the lakor goat population.

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Journal Info

Abbrev

buletinpeternakan

Publisher

Subject

Veterinary Other

Description

Bulletin of Animal Science is published every four months. The Annual subscription rate is Rp. 150.000,-/year. Bulletin receives original papers in animal science and technology which are not published at any other ...