Penbios: Jurnal Pendidikan Biologi dan Sains
Vol 5 No 01 (2020): PENBIOS: Jurnal Pendidikan Biologi dan Sains, Vol. 5, No. 1, Mei 2020 pISSN 2541

Molecular Characterization of Rhizobacteria (Isolate EO-4) as Potential Solvent of Phosphate in-vitro from the Mangrove Ecosystem of Teluk Awur, Jepara-Indonesia

Eka Oktaviani (Unknown)
Rejeki Siti Ferniah (Unknown)
Arina Tri Lunggani (Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang, 50275, Indonesia)



Article Info

Publish Date
21 May 2020

Abstract

Rejuvenation and conservation of mangrove ecosystems, especially the Teluk Awur mangrove ecosystem, Jepara Regency, can be done by using bacteria that are capable of supporting plant growth or called Plant Growth Promoting Rhizobacteria (PGPR). One of the mechanisms that support plant growth by the PGPR group is phosphate dissolving activity, because the phosphate in the soil is in the form of a compound that is difficult for plants to absorb. This study aims to determine the character of Rhizobacter isolates as a superior phosphate solvent in dissolving phosphates in-vitro from the Teluk Awur mangrove ecosystem, namely isolates coded EO-4. These isolates have similar microbiological and biochemical characters to the genus Enterobacter. Molecular characterization of isolates was carried out using the Polymerase Chain Reaction (PCR) method - 16S rRNA sequence analysis (comparing with 16S rRNA sequences in gene banks). The results showed that the phosphate-solubilizing rhizobacteria that were isolated had the same base pair percentage of 48% with Enterobacter pyrinus (access number NR_028875).

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Journal Info

Abbrev

bios

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Public Health

Description

Jurnal Pendidikan Biologi dan Sains (PENBIOS) is a journal which is managed by the Biology Education Study Program at the University Nadhlatul Wathan (UNW) Mataram receiving and publishing article in the form of research (scientific ...