HAYATI Journal of Biosciences
Vol. 28 No. 2 (2021): April 2021

Analysis of Genetic Potential of Banyuwangi Local Rice (Oryza sativa L.) Based on Relative Expression of Homogentisate Geranylgeranyl Transferase (HGGT) and Granule-Bound Starch Synthase I (GBSSI) Gene

Dyati Galuh Pratita (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Tita Putri Milasari (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Elhah Nailul Khasna (Biotechnology Division, Central Laboratory of Mineral and advanced Material, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Sunarmi Sunarmi (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Evi Susanti (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Suharti Suharti (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Delia Wahyu Pangesti (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Annasa Sabatia (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)
Dwi Listyorini (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Malang)



Article Info

Publish Date
01 Apr 2021

Abstract

Rice nutrition including vitamin and amylose contents become important aspect for many people around the world. Rice with high amylose content (low glycemic index) is good for those with Diabetes mellitus. Tocotrienol, one precursor of Vitamin-E biosynthesis is catalyzed by enzymes encoded HGGT, while amylose biosynthesis is catalyzed by enzymes encoded GBSSI. The objective of this study was to find rice varieties with high tocotrienol and/or amylose content based on the expression of HGGT and GBSSI among eight Banyuwangi local rice varieties. Relative expression of HGGT and GBSSI was measured by qRT-PCR and analyzed using 2ΔCt method. Statistical analysis resulted in the significantly different of HGGT and GBSSI relative expression among samples. Relative expression of HGGT from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, Blambangan A3, Merah Bali, Blambangan A2, Berlian, Janur Kuning, and SOJ A3, respectively; while relative expression of GBSSI from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, SOJ A3, Janur Kuning, Berlian, Merah Bali, Blambangan A3, and Blambangan A2, respectively. Based on this research we conclude that Hitam Melik potentially produces higher tocotrienol and lower glycemic index than other studied varieties.

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Journal Info

Abbrev

hayati

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Description

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, ...