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All Journal HAYATI Journal of Biosciences The Journal of Pure and Applied Chemistry Research Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Chemistry Education Practice Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Hydrogen: Jurnal Kependidikan Kimia Jurnal Ilmiah Kesehatan (JIKA) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat

Suharti

Universitas Negeri Malang

Author-ID : 2421015

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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OsDREB2A Gene Polymorphism Does Not Affect Salinity Tolerance Potency of Local Rice Varieties from Banten ‘Ainun Sayyidah Zakiyah; Elhah Nailul Khasna; I Kade Karisma Gita Ardana; Choirun Nita Fikriani; Nina Bunga Anggraini; Dwita Novitasari; Dwi Listyorini; Suharti Suharti
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.123

Indonesia possesses vast coastal agricultural land areas with high salinity. This high salinity is a threat to rice productivity which may decrease to 79.8%. Banten province of Indonesia houses many local rice varieties which have salinity tolerance potency based on its agronomical characters. The adaptability of rice plant in high salinity soil can be affected by OsDREB2A gene, which encodes transcription factors from several salt-tolerant signaling pathway genes. The aims of this study were to find out the possible polymorphism of OsDREB2A gene from Banten local rice varieties. The polymorphism of OsDREB2A analyzed by Single Nucleotide Polymorphism (SNP) and possible molecular structure. OsDREB2A gene was amplified using DNA from Tambleng, Bulu Putih, and Pare Caok varieties as templates through PCR method with a pair of specific primers. DNA fragments obtained were analyzed by means of series of analysis software. From those analysis we obtained 840 bp, 836 bp, and 850 bp gene fragments from Tambleng, Bulu Putih, and Pare Caok varieties, respectively. There is polymorphism of OsDREB2A due to some mutation yet does not massively alter its protein structure. From this study, we suggest that Tambleng, Bulu Putih, and Pare Caok varieties potential for salinity stress resistance genetically.

Analysis of Genetic Potential of Banyuwangi Local Rice (Oryza sativa L.) Based on Relative Expression of Homogentisate Geranylgeranyl Transferase (HGGT) and Granule-Bound Starch Synthase I (GBSSI) Gene Dyati Galuh Pratita; Tita Putri Milasari; Elhah Nailul Khasna; Sunarmi Sunarmi; Evi Susanti; Suharti Suharti; Delia Wahyu Pangesti; Annasa Sabatia; Dwi Listyorini
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.181

Rice nutrition including vitamin and amylose contents become important aspect for many people around the world. Rice with high amylose content (low glycemic index) is good for those with Diabetes mellitus. Tocotrienol, one precursor of Vitamin-E biosynthesis is catalyzed by enzymes encoded HGGT, while amylose biosynthesis is catalyzed by enzymes encoded GBSSI. The objective of this study was to find rice varieties with high tocotrienol and/or amylose content based on the expression of HGGT and GBSSI among eight Banyuwangi local rice varieties. Relative expression of HGGT and GBSSI was measured by qRT-PCR and analyzed using 2ΔCt method. Statistical analysis resulted in the significantly different of HGGT and GBSSI relative expression among samples. Relative expression of HGGT from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, Blambangan A3, Merah Bali, Blambangan A2, Berlian, Janur Kuning, and SOJ A3, respectively; while relative expression of GBSSI from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, SOJ A3, Janur Kuning, Berlian, Merah Bali, Blambangan A3, and Blambangan A2, respectively. Based on this research we conclude that Hitam Melik potentially produces higher tocotrienol and lower glycemic index than other studied varieties.

Characterization of Protease Soluble Collagen (PSC) From Milkfish Scales (Chanos chanos) Nia Lutfiana Lutfiana; Suharti Suharti; Evi Susanti
The Journal of Pure and Applied Chemistry Research Vol 8, No 3 (2019): Edition September-December 2019
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2019.008.03.506

The aim of this study was to characterize protease soluble collagen (PSC) obtained from milkfish scales, extraction using protease from proteolytic bacteria HTcUM7.1 isolate. The characterization included Fourier Transform Infra Red (FT-IR) spectra, Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) profile, Field Emission Scanning Electron Microscopy (FESEM), denaturation temperature by Differential Scanning Calorimetric (DSC) and solubility. The resulting PSC from milkfish scales has white color, fiber with a length of about 20-60 µm, FTIR spectra and SDS-PAGE profile showed that PSC was collagen Type I and denaturation temperature was 145.48 °C, with maximum solubility at pH 1-3 and 1-2 % NaCl. Its high denaturation temperature value allows the collagen to be applied in the fields of medicines and cosmetics.

Surface Behavior of Rhodamin and Tartrazine on Silica-Cellulose Sol-Gel Surfaces by Thin Layer Elution Surjani Wonorahardjo; Anugrah Ricky Wijaya; Suharti Suharti
The Journal of Pure and Applied Chemistry Research Vol 5, No 2 (2016)
Publisher : Chemistry Department, The University of Brawijaya

Physical and chemical interactions are the principles for different types of separation systems as the equillibrium dynamics on surface plays a key-role. Surface modification is a way for selective separation at interfaces. Moreover, synthesis of gel silica by a sol-gel method is preferred due to the homogeneity and surface feature easily controlled. Cellulose can be added in situ to modified the silica features during the process. Further application for to study interaction of rhodamin and tartrazine in its surface and their solubilities in mobile phase explains the possibility for their separation. This paper devoted to evaluate the surface behavior in term of adsorption and desorption of tartrazine and rhodamin on silica-cellulose thin layer in different mobile phase. Some carrier liquids applied such as methanol, acetone, n-hexane and chloroform. The result proves tartrazine and rhodamin is separated and have different behavior in different mobile phase. The retardation factors (Rf) of the mixtures suggest complexity behavior on silica-cellulose surface.

MISKONSEPSI SISWA PADA MATERI LARUTAN BUFFER Widy Ika Parastuti; Suharti Suharti; Suhadi Ibnu
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol.1, No.12, Desember 2016
Publisher : Graduate School of Universitas Negeri Malang

Buffer solution is one high school chemistry material found misconceptions on student understanding. Misconceptions identification method by giving some quetion with same type. Misconceptions identified from recurring student answers incorrectly. The study population was the students of class XI IPA at SMAN 1 Malang academic year 2015/2016 with a research subject in class XI MIA 7 number of 32 students. The results obtained by analysis of four kinds of misconceptions. The cause of the misconception is (1) a weak prior knowledge; (2) problems of symbols and mathematical formulas; (3) difficulty understanding the context of the material; (4) problems in generalize.Larutan buffer merupakan salah satu materi kimia SMA yang ditemukan miskonsepsi pada pemahaman siswa. Metode identifikasi miskonsepsi dengan memberikan beberapa soal yang setipe. Miskonsepsi teridentifikasi dari jawaban salah yang berulang siswa. Populasi penelitian ini adalah siswa kelas XI IPA SMA Negeri 1 Malang Tahun ajaran 2015/2016 dengan subjek penelitian kelas XI MIA 7 sejumlah 32 siswa. Hasil analisis diperoleh empat macam miskonsepsi. Penyebab miskonsepsi adalah (1) pengetahuan awal yang lemah; (2) permasalahan simbol dan rumus matematika; (3) kesulitan memahami konteks materi; (4) permasalahan dalam menggeneralisasikan masalah.

Pengaruh Pembelajaran Inkuiri Terbimbing dengan Penyajian Representasi Submikroskopik yang Berbeda terhadap Pemahaman Konseptual Siswa pada Materi Sel Volta Findiyani Ernawati Asih; Suhadi Ibnu; Suharti Suharti
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 3, No 11: NOVEMBER 2018
Publisher : Graduate School of Universitas Negeri Malang

Abstract: The research was aimed to know the difference of conceptual understanding between two groups who was learned by guided inquiry with static visualization (IT-vis) and guided inquiry with analogy (IT-log) on galvanic cell Quasy experiment which nonequivalent posttet only control group design was used n this research. Learning chemistry must be explicitly to give submicroscopic aspects such as flow of electron and migration of ions. Static visualization visualises directly submicroscopic aspect by giving particulate image, but analogy visualises undirectly submicroscopic aspect by giving verbal information (analog concept). The result of mann whitney u test showed that conceptual understanding between two groups is significantly difference. Mean rank of IT-vis 32,88 is higher than IT-log 28,12. Studets’ conceptual understanding of IT-vis is higher than IT-log on galvanic cell. The difference characteristic of static visualization and analogy can influence students’ conceptual understanding.Abstrak: Penelitian ini bertujuan untuk mengetahui perbedaan pemahaman konseptual siswa setelah melaksanakan pembelajaran inkuiri terbimbing dengan visualisasi statis (IT-vis) dan analogi (IT-log) pada materi sel volta. Jenis penelitian adalah quasy experiment dengan desain nonequivalent posttest only control group. Pembelajaran perlu mengeksplisitkan aspek submikroskopik seperti aliran elektron dan migrasi ion. Visualisasi statis memvisualisasikan aspek submikroskopik secara langsung melalui gambar tingkat partikel, sedangkan analogi memvisualisasikan secara tidak langsung melalui informasi verbal (konsep analog). Hasil uji mann whitney menunjukkan bahwa ada perbedaan pemahaman yang signifikan antara siswa dibelajarkan dengan IT-vis dan IT-log. Nilai mean rank IT-vis 32,88 lebih tinggi daripada IT-log 28,12. Pemahaman siswa IT-vis lebih baik daripada siswa IT-log. Karakteristik yang berbeda antara visualisasi statis dan analogi memengaruhi pemahaman konseptual siswa.

Pemahaman Konsep Siswa Kelas IV melalui Pembelajaran Inkuiri Terbimbing tentang Materi Sifat-Sifat Cahaya Verdiana Somi Koten; Suharti Suharti; Sutopo Sutopo
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 4, No 9: SEPTEMBER 2019
Publisher : Graduate School of Universitas Negeri Malang

Abstract: This study aims to examine the effect of guided inquiry learning models on the understanding concepts of students. This research was conducted at SDK Sta. Maria Assumpta Kupang, NTT. The understanding concepts of the students about the properties of light is measured using multiple choices the questions. The results of the study show that understanding students' science concepts can be improved by using a guided inquiry learning model.Abstrak: Penelitian ini bertujuan untuk meneliti pengaruh model pembelajaran inkuiri terbimbing terhadap pemahaman konsep siswa. Penelitian ini dilakukan di SDK Sta. Maria Assumpta Kupang, NTT. Pemahaman konsep siswa tentang cahaya diukur menggunakan soal tes berbentuk pilihan ganda. Hasil penelitian menunjukkan bahwa pemahaman konsep IPA siswa meningkat dengan menggunakan model pembelajaran inkuiri terbimbing.

Pengembangan Bahan Ajar Tematik Digital untuk Siswa Kelas V Sekolah Dasar Dina Yuli Agustin; Punaji Setyosari; Suharti Suharti
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 5, No 12: DESEMBER 2020
Publisher : Graduate School of Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/jptpp.v5i12.14335

Abstract: The development of digital thematic teaching materials aims to produce a product that is feasible, practical to use, effective for improving the quality of learning, and interesting for students to learn. The results of the development showed a positive impact from the use of the product with an increase in learning activities of 92.14% with the criteria of "very good". Positive results were also shown from the effectiveness of student learning, the pretest value of 60.54 increased significantly in the posttest value of 84.79. In the feasibility analysis obtained a percentage of 86.04% with the criteria "very feasible", practicality 91.07% with the criteria "very practical", and attractiveness get a percentage of 91.89% with the criteria "very interesting". So this digital thematic teaching material can be used in learning.Abstrak: Pengembangan bahan ajar tematik digital ini bertujuan untuk menghasilkan suatu produk yang layak, praktis untuk digunakan, efektif untuk meningkatkan kualitas pembelajaran, serta menarik untuk dipelajari siswa. Hasil pengembangan menunjukkan adanya dampak positif dari penggunaan produk dengan adanya peningkatan aktivitas belajar sebesar 92,14% dengan kriteria “sangat baik”. Hasil positif juga ditunjukkan dari efektivitas belajar siswa, nilai pretest sebesar 60,54 meningkat signifikan pada nilai postest sebesar 84,79. Pada analisis kelayakan diperoleh persentase sebesar 86,04% dengan kriteria “sangat layak”, kepraktisan 91,07% dengan kriteria “sangat praktis”, dan kemenarikan mendapat persentase sebesar 91,89% dengan kriteria “sangat menarik”. Dengan demikian, bahan ajar tematik digital ini dapat digunakan dalam pembelajaran.

EFEKTIVITAS PEMBELAJARAN LC 5E UNTUK MENGURANGI KESALAHAN KONSEP MATERI REDOKS DAN RETENSINYA PADA SISWA KELAS X Noni Asmarisa; Endang Budiasih; Suharti Suharti
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 2, No 9: September 2017
Publisher : Graduate School of Universitas Negeri Malang

This study aims to (1) identify students’ misconceptions in redox (2) determine the effectiveness of LC 5E learning model in correcting student’ misconceptions in redox; and (3) determine its retention after correction of student’ misconceptions in redox with LC 5E learning model. This research is descriptive and pre-experimental. Descriptive study was used to identify and describe the misconceptions students on redox material, while pre-experimental was used to study on the effectiveness of LC 5E learning model in correcting misconceptions and determine its retention. Subject in this study is a science class (X IPA 1 SMA) of Al Muhafidzoh Blitar which consists of 20 students who have learned redox. The misconceptions were observed using a diagnostic test, 15 items of multiple-choice questions with reasoned. The test has validity of 97% and reliability value of 0.934.. The results showed that (1) students has following 14 misconceptions in redox; (2) The LC 5E learning model is effective in improving students' misconceptions in redox reaction with an average reduction of 78.78%; (3) retention of students' understanding of the concept after two weeks of repairs using LC 5E learning model is 95.6%, which is classified in the category very well.Penelitian ini bertujuan untuk (1) mengidentifikasi kesalahan konsep siswa pada materi redoks; (2) mengetahui keefektifan model pembelajaran LC 5E dalam memperbaiki kesalahan konsep redoks; dan (3) mengetahui retensinya setelah dilakukan perbaikan dengan model pembelajaran LC 5E. Penelitian ini bersifat deskriptif dan pra-eksperimental. Penelitian deskriptif digunakan untuk mengidentifikasi dan mendeskripsikan kesalahan konsep siswa pada materi redoks. Penelitian pra-eksperimental digunakan untuk melihat keefektifan model pembelajaran LC 5E dalam memperbaiki kesalahan konsep dan mengetahui retensinya. Subjek penelitian dalam penelitian ini adalah siswa kelas X IPA 1 SMA Al Muhafidzoh Blitar yang berjumlah 20 orang siswa yang telah mendapatkan materi redoks. Data penelitian deskriptif didapatkan dengan menggunakan 15 soal tes diagnostik yang berupa pilihan ganda beralasan sebanyak dengan validitas isi sebesar 97% dan nilai reliabilitas sebesar 0,934. Hasil penelitian menunjukkan bahwa (1) terdapat 14 jenis kesalahan konsep yang dialami siswa pada materi redoks; (2) model pembelajaran LC 5E efektif dalam memperbaiki kesalahan konsep siswa pada materi redoks dengan rata-rata pengurangan kesalahan konsep sebesar 78,78 % dari seluruh siswa; (3) retensi pemahaman konsep siswa setelah dua minggu dari perbaikan menggunakan model pembelajaran LC 5E adalah 95,6% yang diklasifikasikan dalam kategori sangat baik.

Perbedaan Pemahaman Konsep Siswa sebagai Hasil Penerapan Model Pembelajaran LC 5E-Analogi dan LC 5E Gusti Wahyuni; Suhadi Ibnu; Suharti Suharti
Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan Vol 4, No 4: APRIL 2019
Publisher : Graduate School of Universitas Negeri Malang

Abstract: This research aims to see differences in students' conceptual understanding of the reaction rate material as the result of LC 5E-Analogy learning model. LC 5E-Analogy is related to double coding theory, information received by learner is obtained through one of two channels, where LC 5E is expected to facilitate students to obtain information from the enggament phase to the elaboration phase, while the analogy is expected to visualize the submicroscopic aspect, so as to improve understanding of concepts students. This study uses a reasoned multiple choice test instrument that has 91% content validity with a very good category and a reliability coefficient of 0.799 in the high category. The research was conducted in two classes of XI Science at SMAN 9 Malang. Analysis of the data using the Man Whitney U Test, and the Effect Size test to see the meaning of differences in understanding of students' concepts. The results showed that there were differences students understanding in both research classes, where the difference in the values of the two classes (Sig.2-tailed 0.002) and the Effect Size (d = 0.74).Abstrak: Penelitian ini bertujuan untuk melihat perbedaan pemahaman konsep siswa pada materi laju reaksi sebagai hasil penerapan model pembelajaran LC 5E-Analogi. Pemahaman konsep diperlukan siswa untuk memahami materi laju reaksi, sehingga siswa dapat menjelaskan fenomena serta penerapan laju reaksi dalam kehidupan sehari-hari. LC 5E-Analogi berdasarkan theory double coding yaitu informasi yang diterima seseorang diperoleh melalui salah satu dari dua channel, dimana LC 5E diharapkan dapat memudahkan siswa memperoleh informasi dari fase enggament hingga fase elaboration, sedangkan analogi diharapkan dapat memvisualisasikan aspek submikroskopik sehingga meningkatkan pemahaman konsep siswa. Penelitian ini menggunakan instrument tes pilihan ganda beralasan yang memiliki validitas isi 91% dengan kategori sangat baik dan koefisien reliabilitas 0,799 dengan kategori tinggi. Sampel penelitian terhadap dua kelas yang diambil dari keseluruhan kelas XI IPA di SMAN 9 Malang. Analisa data menggunakaan Man Whitney U Test, serta uji Effect Size untuk melihat kebermaknaan perbedaan pemahaman konsep siswa. Hasil penelitian menunjukkan ada perbedaan pemahaman konsep siswa pada kedua kelas penelitian, dimana nilai perbedaan kedua kelas (Sig.2-tailed 0,002) dan nilai Effect Size (d= 0,74) dengan kriteria sedang.

Co-Authors Ali Amirul Mu'minin Almas Dwi Khairana Annasa Sabatia Anugrah Ricky Wijaya Aulanni'am, Aulanni'am Choirun Nita Fikriani Delia Wahyu Pangesti Dina Yuli Agustin Dwi Listyorini Dwita Novitasari Dyati Galuh Pratita Effendy Effendy Elhah Nailul Khasna Endang Budiasih Endang Ciptawati Evi Susanti Fauziatul Fajaroh Findiyani Ernawati Asih Gusti Wahyuni Hendra Susanto I Kade Karisma Gita Ardana I Wayan Dasna Liqanatul Putri Sholikah Nazriati Nazriati dkk. Nia Lutfiana Lutfiana Nina Bunga Anggraini Noni Asmarisa Punaji Setyosari Ricadonna Raissa Suhadi Ibnu Suhadi Ibnu Sunarmi Sunniarti Ariani Surjani Wonorahardjo Sutopo Sutopo Tita Putri Milasari Verdiana Somi Koten Wibi Riawan Widy Ika Parastuti Yudhi Utomo Yuneta Dwi Yunisari ‘Ainun Sayyidah Zakiyah

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