JURNAL KIMIA SAINS DAN APLIKASI
Vol 24, No 2 (2021): Volume 24 Issue 2 Year 2021

Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal

Anceu Murniati (Department of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani Cimahi)
Buchari Buchari (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung)
Suryo Gandasasmita (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung)
Zeily Nurachman (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung)
Arie Hardian (Department of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani Cimahi)
Dera Triani (Department of Chemistry, Faculty of Sciences and Informatics, Universitas Jenderal Achmad Yani Cimahi)



Article Info

Publish Date
15 Mar 2021

Abstract

This research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M pyrrole (Py) and 50-100% PPO apple extract, which is stable using 50 mM of phosphate buffer solution at pH 6.80-7.00 and room temperature. The electrodeposition process is used 400 mesh steel gauze anode ST-304 and carbon plate cathode. Electrodeposition is carried out at potential = 5.00-6.00 V; current = 0.02-0.25 A; the distance from both electrodes = 1.00-2.00 cm for 300-500 seconds. The results from the deposition of PPy/PPO apple extract of the anode are a membrane of mPPy/PPO-apple extract, with total enzyme activity (U) = (957,1441, 2287 and 1754) using 2.00-5.00 mM phenol as a substrate which is measured based on the UV-visible spectrophotometric method. PPy and mPPy/PPO-apple extracts were characterized by SEM and SEM-EDS. The membrane of mPPy/PPO-apple extract can be used to remove phenol in industrial wastewater samples is 50-65% with a filtration capacity of 500 mL for 2 hours.

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Journal Info

Abbrev

ksa

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

Description

urnal Kimia Sains dan Aplikasi (p-ISSN: 1410-8917) and e-ISSN: 2597-9914) is published by Department of Chemistry, Diponegoro University. This journal is published four times per year and publishes research, review and short communication in field of ...