Indonesian Journal of Human Nutrition
Vol. 8 No. 1 (2021)

Uji Deteksi Adulterasi Daging Babi (Sus scrofa domestica) pada Bakso Metode Loop-Mediated Isothermal Amplification

Rahma Micho Widyanto (Department of Nutrition, Faculty of Medicine, Universitas Brawijaya)
Cleonara Yanuar Dini (Unknown)
Irma Sarita Rahmawati (Unknown)
Sekar Ramadhanti Putri (Unknown)
Amelia Nurdini Rozana (Unknown)
Sakinah Hilya Abida (Unknown)
Yunimar Yunimar (Unknown)



Article Info

Publish Date
31 Jul 2021

Abstract

Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to identify the presence of pork DNA in meatballs. Six primers were used for the identification of mitochondrial DNA cytochrome oxidase subunit II. Samples were prepared in the form of mixed beef meatballs containing pork with a concentration of 50; 5; 0.5; 0.05%, respectively. The results showed that the optimal concentration for primers was 4 pmol inner primers, 2 pmol loop primers, and 1 pmol outer primers. The LAMP reaction showed a change in color in the meatball sample with the pork mixture to the lowest concentration. The LAMP technique provides a faster test with the final result of on-the-spot color visualization so that it is potential to be developed as a quick detection kit for identification of halal food processed products

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Journal Info

Abbrev

IJHN

Publisher

Subject

Medicine & Pharmacology

Description

Indonesian Journal of Human Nutrition (IJHN) merupakan jurnal ilmiah yang memuat artikel penelitian di bidang gizi manusia dan di terbitkan oleh Jurusan Gizi Fakultas Kedokteran Universitas Brawijaya Malang dan terbit dua kali dalam setahun (bulan Mei dan ...