The biosynthesis of ZnO nanoparticles and synthesis of chitosan- ZnO nanoparticles were studied. The aims of this research were biosynthesis of ZnO nanoparticles, synthesis of chitosan-ZnO nanoparticles, its characterization and, used as an antibacterial agent of Escherichia coli. ZnO nanoparticles were biosynthesized by reacting with ethanolic extract of guava seeds leaves (Psidium guajava L.) and zinc acetate dihydrate solution. Chitosan-ZnO nanoparticles were synthesized by the heating method. ZnO nanoparticles and chitosan-ZnO nanoparticles were characterized by FTIR spectroscopy and X-ray diffraction, respectively. The suspension of chitosan-ZnO nanoparticles was used as an antibacterial agent with a paper disk method. The result showed that the Zn-O group at ZnO nanoparticles was detected at a wavenumber 615.29 and 673.16 cm-1 The crystallite size of ZnO nanoparticles was 1.43 nm. The wavenumber of 617-655 cm-1 is the Zn-O group at the structure of the chitosan-ZnO nanoparticle. The average of diameter inhibition zone of chitosan-ZnO nanoparticles (1:2) at concentration 0.25 and 0.5 % (w/v) to Escherichia coli was 15.7 ± 1.0 and 18.3 ± 0.4 mm respectively.
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