INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis

Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument

Muhammad Taufiq Hidayat (Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, East Java, Indonesia)
Endry Nugroho Prasetyo (Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, East Java, Indonesia)

Article Info

Publish Date
30 Oct 2021

Abstract

RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires sophisticated and expensive laboratory instruments. In this study, we extract RNA from Staphylococcus aureus bacteria using the PureLink® RNA Mini Kit. The instruments used in this study are basic instruments such as a hand homogenizer and non-thermal centrifuge. The results of RNA extraction were visualized using agarose gel electrophoresis. These results indicate that bacterial RNA extraction can be performed using the PureLink® RNA Mini Kit even with inexpensive basic laboratory instruments.

Copyrights © 2021




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Website

Abbrev

IJMLST

Publisher

Universitas Nahdlatul Ulama Surabaya

Subject

Health Professions Social Sciences

Description

Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument ...