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Contact Name
Gilang Nugraha
Contact Email
gilang@unusa.ac.id
Phone
+6282233441232
Journal Mail Official
ijmlst@unusa.ac.id
Editorial Address
Kota Surabaya, Jawa Timur, Indonesia
Location
Kota surabaya,
Jawa timur
INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 47 Documents
BLOOD LEAD CONCENTRATIONS AND THE NEUROPSYCHOLOGY SCORES OF PREGNANT WOMEN IN KLANG VALLEY, MALAYSIA Shamsul Bahari Shamsudin; Jamal Hisham Hashim; Nik Nasri Nik Ismail; Maharani Pertiwi Koentjoro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1499

Abstract

Pregnant women with high blood lead posed high risk to their fetus as placental transfer can occurs to the fetus. The objective of this study was to identify the relationship between blood lead and the neuropsychological score of women who were in their 3rd trimester of pregnancy. These respondents were undergoing a routine antenatal checkup at a teaching hospital located in Klang Valley areas. Blood lead concentrations were analyzed using graphite furnace Atomic Absorption Spectrophotometer (AAS). The neuropsychological scores were measured with WHO Neurobehavioral Core Test battery (NCTB). The test consists of 7 items, which made up of the Digit Symbol, Trail Making, Digit Span, Benton Visual Retention Test, Pursuit Aiming, Santa Ana Manual Dexterity, Reaction Time and Movement Time tests. The mean blood lead was 7.78±4.77 µg/dL. The mean score for the total NCTB test was 50.00±5.24. Statistical analysis showed blood lead concentrations were inversely correlated with the total NCTB score (r= –0.462, p≤0.01). The correlation was about 21.3%. The General Linear Model (GLM) showed that age (β= –0.15, p=0.017), weight (β=2.67, p=0.05) and height (β=–1.97, p=0.05) also influence the total neuropsychological scores. In conclusion, blood lead reduces the total neuropsychological scores. The scores for each of the 7 items were inversely and significantly correlated with blood lead concentrations except for the Trail Making and Santa Ana Manual Dexterity tests.
Liver Histopathological Change and Malondialdehyde Level of Rattus Norvegicus on Administration of Curcuma Zedoaria and Paracetamol Toxic Dose Putu Oky Ari Tania; Puja Ayu Misuari; Satya Yudhayana; Ketut Ayesha Edelwise Prayoga
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1927

Abstract

High doses of paracetamol create necrosis in the liver and produce free radicals. When liver function decreased in a long time, it will lead to severe liver damage and it will be irreversible. Rhizome of Curcuma zedoaria has the potential effect as an antioxidant, it is assumed that  its properties inhibit the formation of free radicals which formed from toxic doses of paracetamol. The aim of this study was to examine the histological structure of the liver and to determine MDA levels in the administration of C. zedoaria toxic dose and paracetamol on the Rattus norvegicus. The study was used twenty-four rats divided into four groups (positive control: CMC 0.5%; negative control: paracetamol 1.35g/kg body weight; treatment group 1 (T1): C. zedoaria 105 mg/200g and paracetamol 1.35g/kg Body weight 2 hours later, and treatment group 2 (T2): paracetamol 1.35g/ kg Body weight and C. zedoaria 105 mg/200g 2 hours later).  The Kruskall-Wallis test results showed MDA level did not significantly different between groups (p = 0.087). Hepatocellular changes were observed descriptively with Hematoxylin-eosin staining. Positive control showed greater hepatocellular changes rather than other groups, hepatocyte cells were enlarged with cytoplasm showing eosinophilic granules infiltrates, enlarged irregular nuclei, nucleolus prominent, there are many necrosis cells.
Relation of Parasites in Soil with The Existence of Parasites on Farmer's Nails Edza Aria Wikurendra; Merry Crismiati; Globila Nurika
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1850

Abstract

Worms is an infectious disease caused by parasitic worms that can endanger health. Worms that often infect and have a very detrimental impact are soil-borne worm infections or Soil-Transmitted Helminths. Soil-Transmitted Helminths still considered insignificant because it is considered not to cause harm or cause death. This study aims to determine the relationship of parasites in the soil with the presence of parasites on the nails of farmers Sumber Urip 1 Village Wonorejo, East Java, Indonesia. The research method was used observational analytic with a cross-sectional study design which involved 18 Sumber Urip 1 farmers in Wonorejo Village. The sampling technique used was Total Sampling. The bivariate analysis uses Pearson correlation with decision making using significant <0.01. The identification of parasites using the floating method in 18 soil samples contained 12 flattering pieces of hookworm larvae and roundworm eggs. While the results of parasite identification with sedimentation method in 18 nail samples of farmers, there are 11 positive hookworm larvae samples, i.e. Ancylostoma duodenale. The correlation test result showed a relationship between parasites in the soil and nails of Sumber Urip 1 farmers in Wonorejo village (significant as P < 0.01). The use of gloves and footwear (shoes) when working on agricultural land, wash hands with soap and brush nails so that dirt is lost, and consume worm medicine can prevent worms infection.
ASSOCIATION BETWEEN TRIGLYCERIDE SERUM LEVELS AND GLOMERULAR FILTRATION RATE (eGFR) IN PATIENTS WITH CHRONIC RENAL FAILURE AT JEMURSARI ISLAMIC HOSPITAL SURABAYA, INDONESIA Uswatun Hasanah; Suhariyadi; Andreas Putro Ragil Santoso
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1668

Abstract

Chronic Renal Failure (CRF) is a progressive and irreversible decrease in kidney function. One risk factor that affects the progression of CRF is dyslipidemia due to abnormalities of lipid metabolisms. Dyslipidemia is characterized by the increase level of total cholesterol, LDL cholesterol, triglycerides, and a decrease in HDL cholesterol level. eGFR is a parameter for assessing the excretion function, by calculating the amount of filtrate produced by the renal glomerulus. This study aims to determine the relationship between serum triglyceride levels with eGFR in patient suffering from CRF at Jemursari Islamic Hospital Surabaya. This type of research is descriptive experimental with cross–sectional design. The study population was the patients with CRF. The primary data in this study was the results of examination of serum triglyceride levels in patients with CRF. The secondary data (serum creatinine levels, body weight, age, and sex) was obtained from medical records of CRF patients in February 2019. As much as 12% of patients were in the 40–50 years old category, 36% of patients were 51–60 years old, 44% of patients were 61–70 years old, and 8% of patients were 71–80 years old. 72% of patients were male. The mean triglyceride level was 146.68 mg/dL and the average eGFR was 19.86 mL/min/1.73m2. The relationships between triglyceride levels and eGFR was carried out by the Spearman–rho test where r value was –0.442 and value was 0.027. It was concluded that there was a significant negative correlation between serum triglyceride levels and eGFR in patients with CRF.
RETRACTED: N-TERMINAL PRO-BRAIN NATRIURETIC PEPTIDE (NT-proBNP) IN STAGE 1 AND STAGE 2 HYPERTENSION PATIENTS Supriati Wila Djami
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1159

Abstract

This article has been retracted at the request of the Editor in Chief of the Indonesian Journal of Medical Laboratoy Science and Technology (IJMLST) volume 1 number 2 of 2019 pages 65-73 with DOI: 10.33086/ijmlst.v1i2.1159 because it has been published in the Journal of Health Information volume 17 number 1 year 2019 pages 64-74 with DOI: 10.31965/infokes.Vol17.Iss1.232. Papers submitted to IJMLST should neither published previously nor be under consideration for publication another journal. Therefore, the author should make a statement upon submitting. Reusing data in any form must follow good citation principles, therefore this paper has ethical violations in the scientific publishing system. Notice of the revocation of "N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) in Stage 1 and Stage 2 Hypertension Patients" in the Indonesian Journal of Medical Laboratoy Science and Technology volume 3 number 1 in 2021.
ANALYSIS OF APO-B SERUM LEVELS IN BALB/C MICE HYPERCHOLESTEROLEMIC AGAINST TEMULAWAK EXTRACT (Curcuma xanthoriza Roxb) Riyadatus Solihah; M. Shofwan Haris; Yogi Khoirul Abror
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.799

Abstract

APO–B Serum levels is the most predictive value for the incidence of atherosclerosis and coronary heart disease. Curcuma xanthorrhiza Roxb contains curcumin, which can be used as an antioxidant, anti–inflammatory and antihypercholesterol. The mechanism of curcumin contained in ginger to reduce cholesterol is due to its function as a cholagoga or bile stimulant. This study aims to determine the effect of temulawak extract on the levels of APO–B Serum in hypercholesterolemia mice. This research were a true experimental study with a post–test only control group design carried out in February 2018. The extraction As much as 25 mice were divided into 5 groups where are group consisted of 5 mice. Positive control group (K+) were treated with high cholesterol feed and water, negative control group (K–) were given standard feed and water, treatment group 1 (P1) were given high cholesterol food and 25mg/kg BW of curcuma extract for 14 days, treatment group 2 (P2) were treated with foods high in cholesterol and 50mg/kg BW of curcuma extract for 14 days and treatment group 3 (P3) treated with high cholesterol and ginger extract 75mg/kg BW for 14 days. Examination of APO–B levels were measured using the spectrophotometric method. Data were analyzed using One–Way Anova. The results showed that the average of APO–B level at (K+) was 209.7 ± 1.02 mg / dL, at (K–) 115.3 ± 1.04 mg / dL, at (P1) 180.4 ± 1.07 mg / dL, at (P2) 147.6 ± 1.12 mg / dL, at (P3) 119.1 ± 1.10 mg / dL. Based on the results of statistical test it was found that there was a significant decrease in APO–B levels with p–value= 0.001 at alpha 0.05 (p <α).
THE EFFECT OF TEST TUBE STERILIZATION FROM SERUM LIPEMIC AGAINST LEVELS OF TRIGLYCERIDE GPO-PAP METHOD Fitri Fadhilah; Ana Bina Sari; Astika Aprilianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.878

Abstract

In terms of analytic factors, it is important to define acceptable levels of common interferences, such as lipemia or hemolysis. For triglyceride, the laboratory technician must define whether samples with excess lipemia will be included in the study; this depends, in part, on whether the interferences affect the methods. In most laboratories, glass or plastic that is in direct contact associated with bio hazardous material is usually disposable. If not, it must be decontaminated according to appropriate protocols. Immediately rinsing glass or plastic supplies after use, followed by washing with a powder or liquid detergent designed for cleaning laboratory supplies and several distilled water rinses, may be insufficient. To ensure that all remaining fat from lipemic serum that attached to the tube wall has been removed, then the sterilization process is carried out so that a sterile tube is obtained. The purpose of this study is to determine the effect of test tube sterilization from serum lipemic against levels of triglyceride GPO-PAP (Glycerol-3-phosphate oxidase-p-aminophenazone) examination. This research method was a laboratory experiment. We used 8 times repetition with tubes used first are given liquid fat and cleaned by sterilization, washed with surfactant and washed with water only. By using statistical tests ANOVA obtained of this study showed results p>0.05 which is mean the treatment that used did not show a significant difference in the treatment of ordinary water-washed tubes with sterilized tubes and surfactant washed tubes. The conclusion of this study is cleaning of the test tube with the sterilization method is recommended because to avoiding the fear of remaining pollutants that can affect the results, It can also minimize the life of bacteria and viruses from the sample to be examined. However, if the sterilization method is difficult to do because of limited equipment and so on, the use of surfactants and the correct method of cleaning the tube is enough to remove impurities such as fat.
EFFECT OF STARCH AND SULFURIC ACID ON DETERMINATION OF VITAMIN C IN PAPAYA FRUIT USING IODIMETRI Khoirul Ngibad; M Sungging Pradana; Ingrid Retno Y
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.893

Abstract

Vitamin C is an antioxidant that can be used to inactivate oxidation reactions and prevent the formation of free radicals. Sources of vitamin C are fruits, such as papaya fruit. The purpose of this study was to determine the effect of the indicator volume of 1% starch and 2 N sulfuric acid on the determination of vitamin C in papaya fruit samples. This study used the iodimetri method with a standard iodine solution, starch indicator and the addition of sulfuric acid. The variations of starch indicator volume include: 0.25; 0.5, 1, 2 and 3 mL and the variations of sulfuric acid volume include: 0, 2, 4, 6, and 8 mL. The results showed that the optimum 1% starch indicator volume was 1 mL and the optimum volume of 2 N sulfuric acid was 2 mL.
CORRELATION OF MALNUTRITION, WORM INFECTION, PARENTS, INCOME AND KNOWLEDGE ON ANEMIA PREVALENCE AMONG 6-9 YEAR OLD STUDENTS OF LILIBA INPRES ELEMENTARY SCHOOL Marni Tangkelangi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.896

Abstract

Anemia in school–age children will not only cause harm to health but also will have impact on students learning achievement. Thus, anemic children will indirectly affect the national development. The aim of this study is to observe the correlation between malnutrition, worm infection, parents income and knowledge on anemia prevalence among 6–9 years old children. The method of this research was a cross sectional study with a simple random sampling technique, consisted of 222 participants from Liliba Inpres Elementary School. This study was done by measuring children Body Mass Indeks (BMI) ––age to measure the z score and compare to WHO Children growth standard, by using microscope examination with direct method for identifying helminthiasis, by using questionaries to measure the parent knowledge and parent income and also by measuring haemoglobin values using POCT Device. The results showed that there are correlations between malnutrition and worm infection on anemia prevalence (p value 0.000). However, there are no correlations between parent’s knowledge (p value 0.469) and parent’s income on anemia prevalence among 6–9 years old children on Liliba Inpres Elementary School (p value 0.606). In conclusion, these findings confirm that malnutrition and worm infection was correlated with anemia prevalence on Liliba Inpres Elementary School Students so that they are advised to manage their nutritional intake and to practice personal hygiene.
SHAKING RED CAP-BLOOD COLLECTION TUBE WITHOUT ADDITIVE SUBSTANCES IS RECOMMENDED TO ACCELERATE THE BLOOD CLOTTING PROCESS Gilang Nugraha; Rohayati Rohayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 1 (2019): The Value, Importance, and Oversight of Health Research
Publisher : UNUSA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i1.913

Abstract

Many blood collection tube manufacturers do not recommend shaking the red tube. Shaking the red tube to avoid the intensity of the changed of blood that interacts with the glass surface of the tube will trigger the contact path in the coagulation cascade. Generally, the blood takes 30-60 minutes to clots at room temperature without shaking. The aim of this study was to determine the effectiveness of shaking the red-cap blood collection tube in producing serum volume. The method of this study was experimental As much as 5 mL of blood was taken and put into 3 tubes with a volume of 1mL each tube. The first and the second tube were shaken 8 times. The first tube was incubated for 10 minutes while the second tube was incubated for 25 minutes. Meanwhile, the third tube (as a control) was not shaken but was incubated for 40 minutes. The tube were centrifuged at 3000 g for 10 minutes. The serum volume was measured using micropipette and collected into Eppendorf tube. The results showed that there were a difference in the number of serums formed after tube shaking by time variation (P = 0.002), the results of the Post Hoc Test using Bonferroni test while showed that the second tube did not have a difference in serum volume with control (P = 0.751). It can be concluded that the red-cap blood collection tube, which was shaken 8 times and incubated for 25 minutes long could accelerate the coagulation process.