Jurnal Perikanan dan Kelautan
Vol 26, No 3 (2021)

Isolation and Screening of Amylolytic and Proteolytic Thermophilic Bacteria from Way Panas Hot Spring, Kalianda, South Lampung

Mahestri, Laras (Unknown)
Harpeni, Esti (Unknown)
Setyawan, Agus (Unknown)



Article Info

Publish Date
05 Oct 2021

Abstract

Amylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial isolates had hydrolysis activity against protein. The highest activity of amylolitic was achieved by A.WP.50.4 bacteria isolate. Based on the phenotype, biochemistry assay, and 16s rDNA sequencing, the A.WP.50.4 bacteria isolate have high similarity and identified as Bacillus cereusAmylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial isolates had hydrolysis activity against protein. The highest activity of amylolitic was achieved by A.WP.50.4 bacteria isolate. Based on the phenotype, biochemistry assay, and 16s rDNA sequencing, the A.WP.50.4 bacteria isolate have high similarity and identified as Bacillus cereus

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Journal Info

Abbrev

JPK

Publisher

Subject

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Immunology & microbiology Social Sciences

Description

Journal of Fisheries and Marine was published by the Faculty of Fisheries and Marine of the Universitas Riau in 2007. The scope of the research included: Aquaculture, fisheries socioeconomic, fisheries technology, management of aquatic resources, utilization of fisheries and marine ...