Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika
Vol. 4 No. 2: July 2020

Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities.

Dian Puspita Anggraini (Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province, 66137)
Devita Sulistiana (Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province, 66137)
Dwi Kameluh Agustina (Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province 66137)
Almira Ulimaz (Agro-Industry Study Program, Department of Agricultural Industrial Technology, Politeknik Negeri Tanah Laut. Jl. A. Yani Km. 06, Panggung Village, Pelaihari District, Tanah Laut Regency, South Kalimantan Province, Indonesia, Postal code: 70815)

Article Info

Publish Date
27 Jun 2020

Abstract

The purpose of study was to determine kinetic parameters and inhibitory effect of Mg2+ ions on pectinase. Activity test performed at pH 4,5 temperature 55oC for 50' with 10 mm concentrations of each ion 0-10mm. Galacturonic acid content, as product of pectin substrate hydrolysis, was used as basis for determining activity and analyzed by visible spectrophotometry. At concentrations 2 and 4 mm Mg2+ act as an activator, but at concentrations 6, 8, and 10 mM as inhibitors. The value of KM with and without inhibitors is almost same, that is 0.3145% and 0.3105% but value of Vm from both are different, that is 80,645 μg.ml-1.minute-1 and 62,112 μg.ml-1.minute-1. The conclusion that type of inhibitory pectinase with Mg2+ is a non-competitive inhibition. The inhibition constant value (Ki) is 26,84.

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Journal Info
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika
Website

Abbrev

e-Saintika

Publisher

Lembaga Penelitian dan Pemberdayaan Masyarakat

Subject

Education Mathematics Physics Other

Description

Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika was published by Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM) which contains articles that are based on the results of conceptual research and studies in the field of education such as (1) Assessment and Evaluation; (2) Higher ...