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All Journal Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika
Dwi Kameluh Agustina
Biology Education Department, Faculty of Teacher Training and Education, FKIP Islamic University of Blitar, Jl. Majapahit No. 04, Blitar East Java Province 66137
Education Mathematics Physics Other

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Determination of Kinetic Parameters and The Effect of Ion Mg2+ Inhibition Into Pectinase Activities. Dian Puspita Anggraini; Devita Sulistiana; Dwi Kameluh Agustina; Almira Ulimaz
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Vol. 4 No. 2: July 2020
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/e-saintika.v4i2.170

Abstract

The purpose of study was to determine kinetic parameters and inhibitory effect of Mg2+ ions on pectinase. Activity test performed at pH 4,5 temperature 55oC for 50' with 10 mm concentrations of each ion 0-10mm. Galacturonic acid content, as product of pectin substrate hydrolysis, was used as basis for determining activity and analyzed by visible spectrophotometry. At concentrations 2 and 4 mm Mg2+ act as an activator, but at concentrations 6, 8, and 10 mM as inhibitors. The value of KM with and without inhibitors is almost same, that is 0.3145% and 0.3105% but value of Vm from both are different, that is 80,645 μg.ml-1.minute-1 and 62,112 μg.ml-1.minute-1. The conclusion that type of inhibitory pectinase with Mg2+ is a non-competitive inhibition. The inhibition constant value (Ki) is 26,84.
Co-Authors Almira Ulimaz Dian Puspita Anggraini Sulistiana, Devita