Indonesian Journal of Biotechnology
Vol 26, No 4 (2021)

Identification of mercury‐resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene

Wahyu Aristyaning Putri (Biotechnology Laboratory, Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Bulaksumur Yogyakarta 55281, Indonesia)
Hanum Mukti Rahayu (Program of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Pontianak. Jl. Ahmad Yani No. 111 Pontianak 78124, West Kalimantan, Indonesia)
Anis Uswatun Khasanah (Biology Laboratory, Program of Biology, Department of Science, UIN Sultan Maulana Hasanuddin Banten. Jl. Jalan Jendral Sudirman No. 30 Panancangan Cipocok Jaya, Sumurpecung, Kec. Serang, Kota Serang, Banten 42118, Indonesia)
Langkah Sembiring (Microbiology Laboratory, Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Bulaksumur Yogyakarta 55281, Indonesia)
Masashi Kawaichi (Division of Gene Function in Animals, Nara Institute of Science and Technology, Ikoma, 630‐0192, Japan)
Yekti Asih Purwestri (Biochemistry Laboratory, Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Bulaksumur Yogyakarta 55281, Indonesia)

Article Info

Publish Date
31 Dec 2021

Abstract

Streptomyces is one of mercury‐resistant bacteria which can convert Hg2+ into nontoxic Hg0 . This study aimed to identify mercury‐resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small‐scale gold mining (ASGM) area and clone merA gene to the cloning and expression vectors. Molecular identification was conducted using 16s rRNA gene with the maximum likelihood algorithms. Results revealed that the AS1 and AS2 strains were a group of Streptomyces ardesiacus and the BR28 strain was closed to Brevibacillus agri. The AS2 merA gene was cloned to pMD20 cloning vectors, pGEX‐5x‐1 and pET‐28c expression vectors. The transformation was successfully performed in BL21 and DH5α competent cells. The full length of the merA gene was confirmed to be 1,425 bp. This study is the first research on identifying mercury‐resistant Streptomyces and cloning the full‐length merA gene in Indonesia.

Copyrights © 2021




Citation Download
RIS
EndNote, Reference Manager, ProCite
BibTex
Latex, Jabref
Original Source
Download Original
Google Scholar
Check in Google Scholar
Journal Info
Indonesian Journal of Biotechnology
Website

Abbrev

ijbiotech

Publisher

Universitas Gadjah Mada

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...