Jurnal Penelitian Kesehatan
Vol 13 (2022): Nomor Khusus Februari 2022

Daya Hambat Ekstrak Biji Kopi Robusta (Coffea canephora) Asal Bengkulu Terhadap Pertumbuhan Bakteri Penyebab Infeksi Kulit

Anika Rizki (Program Studi Biologi, Fakultas Biologi, Universitas Nasional)
Sri Handayani (Program Studi Biologi, Fakultas Biologi, Universitas Nasional)
Retno Widowati (Program Studi Magister Biologi, Sekolah Pascasarjana, Universitas Nasional
e-mail: retno.widowati@civitas.unas.ac.id (koresponden))



Article Info

Publish Date
26 Feb 2022

Abstract

Bengkulu is one of the provinces in Indonesia which is famous for its superior commodity in the form of robusta coffee (Coffea canephora). The advantages of robusta coffee from Bengkulu continue to be explored, one of which is as a natural antimicrobial ingredient. This study aims to determine the potential antibacterial inhibition of robusta coffee bean extract from Bengkulu, against bacteria that cause skin infections (Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa). The method used was disc diffusion. The inhibitory power of robusta coffee bean extract against test bacteria was measured by the inhibition zone formed. The concentrations of the robusta coffee bean ethanol extract used were 25%, 50%, and 75%. The data obtained were then analyzed using the One-Way Anova test. The results of the analysis showed that the robusta coffee bean extract from Bengkulu with concentrations of 25%, 50%, and 75% had an inhibitory effect on the growth of bacteria that cause skin infections. The higher the concentration of the extract, the larger the diameter of the inhibition zone formed. Robusta coffee bean extract provided the largest inhibition zone for S. epidermidis and S. aureus bacteria starting at a concentration of 50%. Meanwhile, for P. aeruginosa, robusta coffee bean extract gave the largest inhibition zone at a concentration of 75%. The results of the Tukey test subsets showed that there was a significant difference in the inhibitory zone of robusta coffee bean extract on S. epidermidis bacteria with S. aureus and P. aeruginosa bacteria. Meanwhile, for S. aureus and P. aeruginosa bacteria, robusta coffee bean extract had the same inhibition zone.Keywords: bacteria that cause skin infections; robusta coffee beans; inhibitionABSTRAK Bengkulu merupakan salah satu provinsi di Indonesia yang terkenal akan komoditas unggulan berupa kopi robusta (Coffea canephora). Kelebihan kopi robusta asal Bengkulu terus dieksplorasi, salah satunya adalah sebagai bahan antimikrobial alami. Penelitian ini bertujuan untuk mengetahui daya hambat sebagai potensi antibakteri dari ekstrak biji kopi robusta asal Bengkulu, terhadap bakteri penyebab infeksi kulit (Staphylococcus epidermidis, Staphylococcus aureus, dan Pseudomonas aeruginosa). Metode yang digunakan adalah difusi cakram. Daya hambat ekstrak biji kopi robusta terhadap bakteri uji diukur dengan zona hambat yang terbentuk. Konsentrasi ekstrak etanol biji kopi robusta yang digunakan yaitu 25%, 50%, dan 75%. Data yang diperoleh kemudian dianalisis menggunakan uji One-Way Anova. Hasil analisis menunjukkan bahwa ekstrak biji kopi robusta asal Bengkulu dengan konsentrasi 25%, 50%, dan 75% mempunyai daya hambat terhadap pertumbuhan bakteri penyebab infeksi kulit. Semakin tinggi konsentrasi ekstrak, maka semakin besar diameter zona hambat yang terbentuk. Ekstrak biji kopi robusta memberikan zona hambat terbesar pada bakteri S. epidermidis dan S. aureus mulai konsentrasi 50%. Sedangkan pada bakteri P. aeruginosa ekstrak biji kopi robusta memberikan zona hambat terbesar pada konsentrasi 75%. Hasil subsets uji Tukey menunjukkan bahwa terdapat perbedaan yang signifikan zona hambat ekstrak biji kopi robusta pada bakteri S. epidermidis dengan bakteri S. aureus dan P. aeruginosa. Sedangkan pada bakteri S. aureus dan P. aeruginosa, ekstrak biji kopi robusta mempunyai zona hambat yang tidak berbeda.Kata kunci: bakteri penyebab infeksi kulit; biji kopi robusta; daya hambat

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Journal Info

Abbrev

sf

Publisher

Subject

Health Professions Medicine & Pharmacology Nursing Public Health

Description

Journal of Health Research "Forikes Voice" is a medium for the publication of articles on research and review of the literature. We accept articles in the areas of health such as public health, medicine, nursing, midwifery, nutrition, pharmaceutical, environmental health, health technology, clinical ...