Jurnal Pengolahan Hasil Perikanan Indonesia
Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1)

Aktivitas Antioksidan Kolagen dari Kulit Ikan Patin (Pangasius sp.) dengan Enzim Bromelin Kasar Kulit Nanas (Ananas comosus L.): Antioxidant Activity of Collagen from the Skin of Pangas Catfish (Pangasius sp) with Crude Bromelain Enzyme of Pineapple Peel

Fitri Yanti (Politeknik Kelautan dan Perikanan Aceh)
Niken Dharmayanti (Politeknik AUP Jakarta)
Suryanti Suryanti (Peneliti BBRPB Kelautan dan Perikanan)

Article Info

Publish Date
13 Apr 2022

Abstract

Pangas catfish skin is one of fishery by-products containing certain compounds which have the potential to be used as an alternative source of collagen having antioxidant activity. The purpose of this study is to determine the antioxidant activity in collagen, so that it can be used as an antioxidant material. Collagen was made using crude bromelain enzyme from pineapple peel with an activity of 1.0 U/g in several stages, which were pretreatment, deproteinization, degrasing, and extraction, using factorial RAL with a combination of 3 hours, 3.5 hours and 4 hours and crude enzyme concentration of 0 ; 1.5% ; 2%. The results of the observations showed that the best solubility was obtained in the T3E2 treatment which was 98.96%. The best viscosity was 22.67 cP. The best acidity (pH) at T1.E2 was 6.73. The molecular weights α1, α2, β and ƴ were : 131.51 kDa, 110.48 kDa, 202.48 kDa and 243.93 kDa. The best antioxidant activity was in T1.E3 treatment, which was 20.45 ferrous sulfate/g.

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Journal Info
Jurnal Pengolahan Hasil Perikanan Indonesia
Website

Abbrev

jphpi

Publisher

Institut Pertanian Bogor

Subject

Agriculture, Biological Sciences & Forestry

Description

JPHPI publishes manuscripts in the field of marine post-harvest, aquatic biotechnology, aquatic biochemistry, aquatic product diversification, and characteristic of aquatic raw materials. In addition, JPHPI also publishes research about aquatic product quality, standardization, and other researches ...