TEKNOLOGIA
Vol 4 No 2 (2022): Jurnal Teknologia

EKSTRAKSI KOLAGEN DARI KULIT IKAN SELAR (Selaroides Leptolepis) DENGAN MENGGUNAKAN METODE LARUT ASAM DENGAN PENAMBAHAN ENZIM PEPSIN DAN PAPAIN

Ayu Dahliyanti (Universitas Pertamina)
Nur Indah Shifani (Universitas Pertamina)

Article Info

Publish Date
02 Apr 2022

Abstract

Pepsin soluble collagen (PSC) and papain soluble collagen (PaSC) were successfully extracted from the skin of Yellowstripe fish (Selaroides Leptolepis) through the combination of acid and enzymatic method. Collagen was obtained through a four-stage process which consisted of sample preparation, pretreatment, enzymatic extraction and salting out. Extraction was carried out for 12 hours and 24 hours by adding different doses of pepsin and papain enzymes. The highest yield of PSC and PaSC were 18.64% and 4.140% by mass. The as-prepared collagen were then analyzed for moisture content and ash content. Water content from PSC and PaSC were respectively 2.37% and 3.68% and the ash content was 15.87% and 35.89%, respectively. Fourier Transform Infrared (FTIR) analysis for both sample showed that the triple helix structure of collagen could be maintained even after the extraction process. From Differential Scanning Calorimetry (DSC) analysis, it was found that PSC and PaSC denaturation temperatures were of 64.05°C and 82.29°C.

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Journal Info
TEKNOLOGIA
Website

Abbrev

teknologia

Publisher

Aliansi Perguruan Tinggi Badan Usaha Milik Negara

Subject

Automotive Engineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Engineering Mathematics

Description

Jurnal Teknologia dengan ber-ISSN 2654-5683 merupakan bagian dari Aliansi Perguruan Tinggi Badan Usaha Milik Negara (APERTI BUMN), adalah jurnal keilmuan yang merupakan produk kolaborasi dari lima (5) Perguruan Tinggi yang tergabung dalam APERTI BUMN, yaitu Universitas Pertamina, Telkom University, ...