Indonesian Journal of Chemistry
Vol 22, No 2 (2022)

Metabolite Profiling of Ebony (Diospyros celebica Bakh) Leaves and Wood Extracts Using LC-MS/MS

Dien Atin Boritnaban (Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia)
Alfi Hudatul Karomah (Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia)
Dewi Anggraini Septaningsih (Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia)
Muhammad Majiidu (Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia)
Fifi Gus Dwiyanti (Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia Department of Silviculture, Faculty of Forestry and Environment, IPB University, Jl. Lingkar Akademi)
Iskandar Zulkarnaen Siregar (Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Kampus IPB Dramaga, Bogor 16680, Indonesia Department of Silviculture, Faculty of Forestry and Environment, IPB University, Jl. Lingkar Akademi)
Mohamad Rafi (Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia Advanced Research Laboratory, Institute of Research and Community Services, IPB University, Jl. Palem Raya Ka)



Article Info

Publish Date
08 Mar 2022

Abstract

Ebony (Diospyros celebica Bakh) is an endemic forest tree species found in Sulawesi whose basic information on its metabolite profile is still lacking. The objective of this research was to separate and identify putatively metabolite present in the leaves and wood of ebony across Sulawesi. Separation and identification of ebony metabolites were carried out using UHPLC-Q-Orbitrap HRMS analysis. Using ultrasonication with ethanol as the extracting solvent, we used powdered ebony leaves and wood. The results showed that the metabolites contained in the leaves and wood of ebony were 59 metabolites. About 14 compounds were found in the leaves and wood, 21 compounds in the wood, and 24 compounds in the leaves. The identified metabolites are flavonoids, terpenoids, amino aldehydes, alkaloids, quinones, steroids, amino acids, fatty acids, and saccharides. Clustering of ebony using principal component analysis obtained leaves and wood groups using peak area of known compounds as the variable.

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Journal Info

Abbrev

ijc

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...