Kaunia: Integration and Interconnection of Islam and Science Journal
Vol. 10 No. 2 (2014)

SELEKSI BAKTERI TERMOFILIK SELULOLITIK PASCA ERUPSI MERAPI

Anna Rakhmawati (Jurusan Pendidikan Biologi, FMIPA, Universitas Negeri Yogyakarta)
Evy Yulianti (Jurusan Pendidikan Biologi, FMIPA, Universitas Negeri Yogyakarta)
Eli Rohaeti (Jurusan Pendidikan Kimia, FMIPA, Universitas Negeri Yogyakarta)



Article Info

Publish Date
31 Oct 2014

Abstract

Thermophilic bacteria after the eruption of Merapi in 2010 have been isolated from the Gendol Atas river with dilution and enrichment methods. The purpose of this study was to conduct the selection of these bacteria as producers of cellulase enzymes. Selection was done by growing 348 bacterial isolates on selective media Mandels-CMC 0.5% using Carboxy Methyl Cellulose (CMC) as carbon source. Incubation was performed at 55 °C for 24 hours. The results showed 255 isolates (73.27%) were able to grow in Mandels medium-CMC 0.5%. Bacterial isolates were isolated by enrichment method (69.8%) were more able to grow on selective media compared with the dilution method (30.2%). The diameter of the three largest colonies were isolates D13a (2.44 cm); E135 (2.33 cm); and D110a (1.98 cm).

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Journal Info

Abbrev

kaunia

Publisher

Subject

Religion Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics

Description

The aim of KAUNIA journal is to publish high-quality articles dedicated to all aspects of the latest outstanding developments in the field of Science, Mathematics, Education, Technology, and Religious Perspective Interconnect-Integration. The journal publishes articles, reviews, and research notes ...