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All Journal Jurnal Penelitian Saintek Jurnal Sains Dasar Jurnal Keolahragaan Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Pada Masyarakat Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Molekul: Jurnal Ilmiah Kimia Kaunia: Integration and Interconnection of Islam and Science Journal Jurnal Pendidikan Progresif Jurnal Edukasi Biologi Kingdom (The Journal of Biological Studies)

Evy Yulianti

Universitas Negeri Yogyakarta

Author-ID : 2977417

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

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THE OPTIMATION OF CELLULASE ENZYME OF MOLD ISOLATED FROM AGRICULTURE LAND IN WUKIRSARI AFTER MERAPI ERUPTION Umniyatie, Siti; Rakhmawati, Anna; Yulianti, Evy
Jurnal Sains Dasar Vol 4, No 1 (2015): April 2015
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

The aim of this study was to develop potency of mold isolated from agriculture land after Merapi eruption in 2010 at Wukirsari, Cangkringan, Sleman, Yogyakaerta. The treatment were temperature and pH optimation to the cellulase enzyme activity and the protein level yield by mold isolate A 2.10, A 2.15 and B 3.18 in different substrate, avicel and carboxymethyl cellulose (CMC). This study was experimental study to find out the substrate, pH and temperature which yield the best activity and protein level from mold isolate A 2.10, A 2.15 dan B 3.18. the population of this study all of the crude enzymes from mold isolate, and the sample was 1 ml of crude enzyme which treated with different substrate, pH and temperature. The result of this study showed that the best susbtrate was avicel and the optimum temeperature and pH for isolate A.2.10 was 25 ⁰C Keywords: optimation, cellulase enzyme, cellulolytic

MICROALBUMINURIA ON DIABETES PATIENTS MELLITUS TYPE 2 HYPERTENSIVE Evy Yulianti
Jurnal Penelitian Saintek Vol 14, No 1: April 2009
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Diabetes mellitus (DM) is a metabolic aberration that can cause some complications, including the occurrence of diabetic nephrophaty (DN). Patients with type 2 DM usually have hypertension likely to double compared to the non-DM patients. Prevalence of hypertension is usually greater in patients with type 2 DM with increased albumin in their urine. Data estimate that microalbuminuria is a value as the index of vaskuler damage, especially in the DM and hypertension. The objective of this research is to investigate microalbumin in type 2 DM patients normotensif and type 2 DM patients hypertensif and assess the influence of blood pressure as risk factors in the occurrence of DN in type 2 DM patients. Subjects of this study are 87 people with type 2 DM normotensif and hipertensif consisting of 54 ND patients and 33 non ND. Microalbumin is measured with the dipstick microalbumin and blood pressure with a sphygmomanometer. The comparison between the control group who examined was showed with X2 with two degrees of freedom with significance level (alpha) 0.05 and odds ratio calculation is done. Results obtained in this research is people with type 2 DM hypertensif have a higher frequency in the DN group (67.3%) compared to the non-DN (p = 0.043) with OR = 2.64. Conclusion of this research is the positive result of the microalbumin examination is bigger in the people with type 2 DM hypertensif and hypertension is a risk factor for occurrence of ND in type 2 DM patients

EXPLORATION OF THERMOFILIC BACTERIA POST ERUPSI MERAPI AS EXTRACELULAR ENZYME MANUFACTURER Anna Rakhmawati; Evy Yulianti
Jurnal Penelitian Saintek Vol 17, No 1: April 2012
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian mengenai bakteri termofilik pasca erupsi Merapi masih sangat terbatas padahal masih banyak potensi yang dapat diteliti, salah satunya mengenai enzim ekstraseluler yang dihasilkannya. Tujuan penelitian ini adalah untuk isolasi, karakterisasi dan memperoleh isolat bakteri termofilik yang mampu menghasilkan enzim ekstraseluler. Isolasi bakteri termofilik dilakukan dengan menggunakan 2 (dua) metode yaitu dilution dan enrichment dengan media Nutrient Broth kemudian diinkubasi pada 55 ºC, dilanjutkan dengan seleksi pada suhu 70 ºC. Karakterisasi yang dilakukan, yaitu karakterisasi morfologi koloni. Setelah itu dilakukan skrining aktivitas enzim amilase, protease, dan selulase. Hasil penelitian menunjukkan bakteri termofilik pasca erupsi Merapi yang diisolasi dari sampel air dan pasir Kali Gendol Atas dengan suhu inkubasi 55⁰C diperoleh 480 isolat, setelah diseleksi pada suhu 70 ⁰C diperoleh 253 isolat. Karakter fenotipik isolat bakteri termofilik pasca erupsi Merapi menunjukkan keanekaragaman morfologi koloni meliputi warna, bentuk, ukuran, tepi, dan elevasi koloni. Isolat bakteri termofilik yang menghasilkan enzim ekstraseluler amilase sebanyak 9 isolat, enzim protease sebanyak 4 isolat, dan 1 isolat penghasil enzim selulase pada suhu inkubasi 70 ⁰C .Kata kunci: bakteri, termofilik, enzim ekstraseluler

OPTIMATION OF ANTIMICROBIAL SUBSTANCE PRODUCTION IN CELL FREE EXTRACT FROM THERMOPHYLIC BACTERIA FERMENTATION AFTER MERAPI ERRUPTION Evy Yulianti; Anna Rakhmawati; Kartika Ratna Pertiwi
Jurnal Sains Dasar Vol 4, No 2 (2015): October 2015
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

The aims of this study was to investigate the effect of medium with different pH (6,7,9), salt concentration (0,5; 1; 2 %) and fermentation periode (24 and 48 hr) to the antimicrobial activity of cell free extract to three pathogens, Eschericia coli, Staphylococcus aureus, and Candida albican. This study consists of antimicrobial compounds production with different medium and continued by antimicrobial tested to fungi and bacterial pathogens Eschericia coli, Staphylococcus aureus, and Candida albican by Kirby Bauer method with paper disk. Different salt concentration, pH and fermentation periode affected the antimicrobial activity potency of cell free extract yielded by thermophylic bacteria. Treatment which yielded CFE with the best antimicrobial activity was treatment with 24 hr fermentation, pH 7 and salt concentration 2% to S aureus and pH 6 salt concentration 1% to E coli. Cell free extract had no potency as antifungi to Candida albicans except CFE yielded by thermophylic bacteria fermented in medium with pH 7 and salt concentration 1% in 24 hr with inhibition zone index 1,17. Keywords: cell free extract, antimicrobial, pH, salt concentration, fermentation period

Isolasi dan uji aktivitas enzim lipase termostabil dari bakteri termofilik pasca erupsi Merapi Drajat Pramiadi; Evy Yulianti; Anna Rakhmawati
Jurnal Sains Dasar Vol 3, No 1 (2014): April 2014
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

The usage of enzyme which comes from microorganism is more advantageous because it is easier to be multiplied in a relatively short time and does not need a large space, including lipase enzyme, which is often used in industries. The objective of this research is to isolate thermo stable lipase enzyme from thermophilic bacteria and to understand specific ativities. The thermophilic bacteria isolation is done using pour plate method. Selection of the bacteria producing lipase is shown from the presence of the biggest translucent zone around the colony on the olive oil medium with 0.1% Rhodamine-B. Two isolate produced by the biggest translucent zone are then continued by lipase enzyme isolation and activity tests. From 253 isolate, 43 isolate can grow on the medium that contains olive oil and 2 isolate shows translucent zone around the isolate, that is isolate 361 and isolate 213. The growth curve measurement for 24 hours shows that the exponential time is reached at the 10th hour for both isolates. The result of this research shows that temperature, pH, and enzyme isolate variation effects the enzyme activities. The enzyme activity tests show that on isolate 361, the best enzyme activities is obtained from incubation temperature of 50⁰C with pH 7, whereas for isolate 213, the best enzyme activities is reached when incubation temperature is 70⁰C with pH 6. Key words: lipase, olive oil, thermophilic bacteria, thermo stable enzyme, enzyme activities

UTILIZATION PROBIOTIC LACTIC ACID BACTERIA FISH FROM WASTE FAECES ON LDL LEVEL BLOOD BROILER CHICKENS STRAIN LOHMANN Astuti Astuti; Siti Umniyati; Anna Rakhmawati; Evy Yulianti
Jurnal Sains Dasar Vol 5, No 1 (2016): April 2016
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Abstract This study aims to determine the effect of lactic acid bacteria supplement from sewge force feeding fish to the LDL content of broiler chicken blood. The research samples are 40 of 1 day old broiler chicken from PT. Multi Breeder Adiram, which were divided into 4 treatments, were taken randomly and maintined for 42 days. Data were analyzed by analysis of variance using a completely randomized design unidirectional pattern, followed by a test of Duncan's Multiple Range Test (DMRT).Probiotic Lactic Acid Bacteria Isolates treatment (BAL) were used in this study is the bacterium Streptococcus thermopillus in the form of freeze drying from the Laboratory of Nutritional Biochemistry, Faculty of Animal Husbandry, UGM. I as a control treatment (without BAL) Treatment II BAL cell count was 106 CFU / ml., The third treatment is the number of BAL Cells are 107CFU / ml. , IV treatment BAL cell count was 108 CFU /ml. The results showed that there is no significant effect of BAL isolate addition on the LDL level of the broiler chicken blood. Keywords: lactic acid bacteria, LDL, freeze drying

SELEKSI BAKTERI TERMOFILIK SELULOLITIK PASCA ERUPSI MERAPI Anna Rakhmawati; Evy Yulianti; Eli Rohaeti
Kaunia: Integration and Interconnection Islam and Science Vol. 10 No. 2 (2014)
Publisher : Fakultas Sains dan Teknologi UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/kaunia.1070

Thermophilic bacteria after the eruption of Merapi in 2010 have been isolated from the Gendol Atas river with dilution and enrichment methods. The purpose of this study was to conduct the selection of these bacteria as producers of cellulase enzymes. Selection was done by growing 348 bacterial isolates on selective media Mandels-CMC 0.5% using Carboxy Methyl Cellulose (CMC) as carbon source. Incubation was performed at 55 °C for 24 hours. The results showed 255 isolates (73.27%) were able to grow in Mandels medium-CMC 0.5%. Bacterial isolates were isolated by enrichment method (69.8%) were more able to grow on selective media compared with the dilution method (30.2%). The diameter of the three largest colonies were isolates D13a (2.44 cm); E135 (2.33 cm); and D110a (1.98 cm).

Preparation and Antimicrobial Activity Analysis of Organic Soap Bar Containing Gnetum gnemon Peel Extract Sri Handayani; Indyah Sulistyo Arty; Cornelia Budimarwanti; Karim Theresih; Evy Yulianti; Melati Khairuddean
Molekul Vol 16, No 3 (2021)
Publisher : Universitas Jenderal Soedirman

Melinjo (Gnetum gnemon L.) peel has been known as antioxidant, antibacterial, and antifungal, but not yet used as cosmetic ingredients or drugs optimally. This research focuses on the production of organic soap bars from melinjo peel extract, the quality testing, and antimicrobial analysis against selected microbes. The soap making process was done by the saponification reaction between the mixture of palm oil and coconut oil with sodium hydroxide through cold and hot processes. Distilled water and ethanol were used as solvents for melinjo peel extraction. The quality test was done by determining insoluble fraction in ethanol, free alkali, and unsaponified fatty matter. An antimicrobial activity test was conducted using the Kirby-Bauer diffusion method. The result shows that the quality test fit with INS 2016 for all the criteria except for unsaponified fatty acid in the cold process soap bar. Inhibition zone of organic soap bar sequentially decreased against the growth of Tricophytone mentagrophytes, Staphylococcus aureus, and Staphylococcus epidermidis. Therefore, the organic soap bar with melinjo peel extract shows a good potential to be used as an antimicrobial soap.

Pengembangan Potensi Joglo dan Gamelan sebagai Tujuan Wisata Budaya Rela Adi Himarosa; Cahyo Setiadi Ramadhan; Evy Yulianti
Jurnal Pengabdian Pada Masyarakat Vol 4 No 3 (2019)
Publisher : Universitas Mathla'ul Anwar Banten

POTENTIAL EXPANSION OF JOGLO AND GAMELAN AS CULTURAL TOURISM DESTINATIONS. Joglo is one of the heritage forms of traditional Javanese houses in Yogyakarta, as well as traditional Gamelan musical instruments. The former Head of the Gilangharjo Village Jetis, Gilangharjo, Pandak, Bantul opened a Joglo with a set of Gamelan music equipment. Joglo has been approved as a cultural heritage building by the DIY Provincial Government. Even though Joglo has become a cultural heritage building, the potency for cultural heritage houses and Gamelan musical instruments are underutilized by residents. The development of Joglo cultural tourism and Gamelan will run well by involving the village community. This service aims to empower the community to increase the potency of Joglo and Gamelan to become cultural tourism destination and become community-based tourism. The purpose of this service are to participate by involving the village community involved in tourism development and starting to become known as the Joglo location and Gamelan arts in Jetis Village. The results of this service consist of profiles of Joglo websites and videos. Utilizing the advances in information technology and social media, the more popular Joglo in Jetis RT 03.

Pelatihan Identifikasi Potensi Hazard Bahan Pangan Sebagai Upaya Pencegahan Keracunan Jajanan Anak Sekolah Anna Rakhmawati; Siti Umniyatie; Evy Yulianti
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 1, No 2 (2017)
Publisher : Yogyakarta State University

Kasus keracunan karena mengkonsumsi jajanan anak sekolah masih banyak dijumpai di tingkat sekolah dasar. Target pelaksanaan Program Pengabdian kepada Masyarakat yaitu terjadinya peningkatan pengetahuan dan keterampilan guru dan karyawan SD Muhammadiyah Sleman dalam mengenali dan mengidentifikasi potensi hazard (cemaran) bahan pangan; meningkatkan keterampilan dalam memilih bahan pangan yang aman pada jajanan anak sekolah. Kemudian mendampingi dan memotivasi kader guru untuk mengimplementasikan bekal pengetahuan dan keterampilan yang sudah diperoleh dalam mencegah keracunan jajanan anak sehingga jajanan yang dikonsumsi bergizi seimbang, sehat dan higienis. Tahap persiapan meliputi audiensi, koordinasi, dan pemantapan program dengan mitra kemudian identifikasi peserta kegiatan. Tahap pelaksanaan yaitu kegiatan seminar berupa pemberian materi dan pelatihan. Materi disampaikan oleh tim pengabdi dilanjutkan pelatihan keterampilan mengkarakterisasi potensi cemaran baik fisik, kemis maupun biologis pada jajanan anak serta upaya pencegahannya. Kegiatan terakhir yaitu evaluasi dan perbaikan. Hasil program menunjukkan keberhasilan proses yang ditunjukkan dengan kehadiran peserta mencapai 100%, peningkatan hasil tes pengetahuan sebesar 20,47 %, dan sebanyak 90% peserta telah memiliki keterampilan dalam memilih bahan pangan yang aman pada jajanan anak sekolah.

Co-Authors Absari Hanifah Ahmad Kamal Sudrajat Anna Rahmawati Anna Rakhmawati Anna Rakhmawati Anna Rakhmawati Anna Rakhmawati Anna Rakhmawati Astuti Astuti Cornelia Budimarwanti Dinnisa Haura Zhafira Hidayat Drajat Pramiadi Eli Rohaeti Erna Setyaningrum Fadli Robby Al-Farisi Fajarotul Hidayah Fitria Maria Ulfha Hairiyah Heru Nurcahyo Himarosa, Rela Adi Hosniyah Indyah Sulistyo Arty Ixora Sartika Mercuriani Karim Theresih Kartika Ratna Pertiwi Kartika Ratna Pertiwi Lili Sugiyarto Melati Khairuddean Muhammad Iqbal Perdana Nida Husna HR Ramadhan, Cahyo Setiadi Renny Septiana Siti Umniyati Siti Umniyatie Siti Umniyatie Siti Umniyatie Sri Handayani Suratsih Suratsih Tri Endah Nursanti Tzou-Chi Huang Ukhti Jamil Rustiasari Veronica Deavina Wispar Sunu Bram Dwandaru

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