Jurnal Medik Veteriner
Vol. 4 No. 1 (2021): April

Immunohistochemical Detection of Coxiella burnetii in Cattle Spleen Organs from Ampel Slaughterhouse, Boyolali Regency

Eko Prasetyo Nugroho (Sekolah Pascasarjana IPB, Program Studi Ilmu Biomedis Hewan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor)
Agus Setiyono (Laboratorium Patologi, Divisi Patologi Veteriner IPB, Fakultas Kedokteran Hewan, Institut Pertanian Bogor)
Upik Kesumawati Hadi (Laboratorium Entomologi, Divisi Parasitologi dan Entomologi Veteriner IPB, Fakultas Kedokteran Hewan, Institut Pertanian Bogor)
Wiwin Winarsih (Laboratorium Patologi, Divisi Patologi Veteriner IPB, Fakultas Kedokteran Hewan, Institut Pertanian Bogor)
Dwi Astuti (Pusat Penelitian Biologi, Divisi Zoologi, Lembaga Ilmu Pengetahuan Indonesia Bogor)

Article Info

Publish Date
15 Apr 2021

Abstract

Q-fever is a zoonotic bacterial disease that caused by Coxiella burnetii. These microorganism are gram negative and obligate intracellular bacteria. This study was conducted to detect C. burnetii in cattle organs which collected from Ampel slaughterhouse Boyolali Regency. In this study, spleen, heart, lung, liver and kidney were collected from 100 cattle. The samples were tested by immunohistochemical (IHC) method using polyclonal anti- C. burnetii antibodies. Immunohistochemical examination found the presence of C. burnetii in the cytoplasm of macrophage cells with specific brown color only in the spleen as many as 4 out of 100 cattle showing immunoreactive (4%). The four positive individual samples were from Simental cattle. These results indicate that Q-fever was found in local cattle in Boyolali Regency.

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Journal Info
Jurnal Medik Veteriner
Website

Abbrev

JMV

Publisher

Universitas Airlangga

Subject

Biochemistry, Genetics & Molecular Biology Veterinary

Description

urnal Medik Veteriner (JMV) publishes high quality and novelty papers focusing on Veterinary and Animal Science. The fields of study are anatomy, pathology, basic medicine, veterinary public health, microbiology, veterinary reproduction, parasitology, animal husbandry and animal welfare. Food ...