Livestock and Animal Research
Vol 19, No 3 (2021): Livestock and Animal Research

Keragaman genotipik entok (Cairina moschata) berdasarkan polimorfisme protein darah

Sutopo Sutopo (Program Studi Peternakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, 50275)
Dela Ayu Lestari (Program Studi Peternakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, 50275)
Asep Setiaji (Program Studi Peternakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, 50275)
Edy Kurnianto (Program Studi Peternakan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang, 50275)



Article Info

Publish Date
17 Nov 2021

Abstract

Objective: The aim of this study was to identified genetic diversity of muscovy duck in Central Java through blood protein polymorphisms by using electrophoresis method.Methods: Blood sample was collected from a total of 60 muscovy ducks from the districts of Demak, Magelang and Pekalongan, 20 samples each. The Electrophoresis Polyacrylamide Gel of Electrophoresis–Thin Layer Electrophoresis (PAGE-TLE) was used in this study. Parameters observed were protein of albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and amylase-I (Am-I). Gene frequency, heterozygosity (Ho) and genetic distance were analyzed by using DISPAN program.Results: The results showed that the Alb, Cp, Tf and Am-I of muscovy duck showing polymorphic characters. Gene frequency of AlbB (0.61) was higher compared to AlbA (0.39). Gene frequency of CpA (0.62) was higher than the CpB (0.38). Furthermore, gene frequency of TfA and TfB were 0.32 and 0.68, respectively as well as gene frequency of Am-IA (0.82) was higher than Am-IB (0.18). Muscovy duck population of Demak showed higher Ho value for Alb and Am-I, whereas Magelang showed higher value for Cp and Tf. Population of muscovy duck of Demak showed closer genetic distance to Pekalongan than Magelang.Conclusions: The protein blood locus of three population of muscovy duck in Central Jawa showed polymorphic characters.

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Journal Info

Abbrev

lar

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

Description

Livestock and Animal Research (Livest. Anim. Res.) publishes original research article in the field of livestock and other animal research. This journal previously titled as Sains Peternakan: Jurnal Penelitian Ilmu Peternakan and changed into Livestock and Animal Research in order to broaden the ...