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All Journal Jurnal Veteriner Jurnal Studi Manajemen Organisasi Jurnal Sain Peternakan Indonesia Journal of the Indonesian Tropical Animal Agriculture Livestock and Animal Research Tropical Animal Science Journal Sinov : Media Informasi Penelitian Kabupaten Semarang Jurnal Teknologi Pangan Livestock and Animal Research

Sutopo Sutopo

Department Of Animal Science, Faculty Of Animal And Agricultural Science, Diponegoro University

Author-ID : 2939356

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Energy Library & Information Science Social Sciences Veterinary

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Phe Keragaman Fenotipe Sapi Peranakan Ongole di Wilayah Sumber Bibit di Jawa Tengah (PHENOTYPE DIVERSITY OF ONGOLE GRADE CATTLE IN BREEDING AREA OF CENTRAL JAVA) Agus Tri Sudaryanto; Sutopo Sutopo; Edy Kurnianto
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The eefforts to maintain and improve of the genetic quality of Ongole Grade (OG) in the breeding area of Central Java Province is low implemented, and it is feared that the quality of OG has decreased. One effort to maintain the superiority of genetic by identifying and studying the specific characters by female cattle. The objective of this study was to analyze the diversity of Ongole Grade (OG) grade body weight and morphology in three regencies of Central Java Province. Two hundred and twenty heads of OG cattle female belonging to farmer were used as a study object consisted of 75 heads, 75 heads and 70 heads of cattle come from Rembang Regency, Blora Regency and Kebumen Regency, respectively. The samples were determined by purposive sampling. Body measurements measured were shoulder height (SH), body length (BL), chest circumference (CC), pelvic height (PH), chest width (CW) and chest depth (CD). Body weight (BW) is calculated using a formula Schrool. Data were analyzed by using the Statistical Analysis System (SAS) ver. 6.12. The quantitative characteristics observed of female OG cattle in Rembang, Blora and Kebumen regencies showed diverse. The value of diversity is 3.2 - 11.2%. Body weight, body length, chest circumference and pelvic height showed significantly different (P<0.05). Average of body weight and body measurement of OG cattle in Kebumen are greater than Rembang and Blora regencies. Genetic relationships on the basic body weight and body measurements showed that female OG cattle in Rembang and Blora regencies had a closer relationship than that of to Kebumen regency.

PERFORMA EKSTERIOR GENETIK DAN REPRODUKSI INDUK DOMBA PROLIFIK DI KECAMATAN BAWEN DAN JAMBU KABUPATEN SEMARANG JAWA TENGAH Sutiyono; E.T. Setiatin; Sutopo; Y.S. Ondho; E. Kurnianto
Media Informasi Penelitian Kabupaten Semarang Vol. 3 No. 2 (2021): Desember: Media Informasi Penelitian Kabupaten Semarang
Publisher : Badan Perencanaan, Penelitian Dan Pengembangan Daerah, Kabupaten Semarang

Penelitian di Kecamatan Bawen dan Jambu Kabupaten Semarang dilaksanakan pada bulan Mei- September 2009. Hasil penelitian sudah dipublikasikan menjadi lima judul aspek keilmuan di media publikasi. Artikel ini merupakan rangkuman dari tiga publikasi yang sesuai dengan judul makalah, tentang eksterior, genetik dan reproduksi induk domba prolifik. Tujuan penelitian untuk mengetahui performa eksterior, genetik dan reproduksi yang dapat digunakan sebagai dasar seleksi induk domba prolific yang baik. Penelitian dilakukan menggunakan metode survey dengan peternak sebagai responden dan induk dombanya sebagai materi penelitian. Penentuan materi secara porpusive sampling yaitu induk yang sudah pernah beranak minimal 3 kali. Materi dibagi menjadi tiga kelompok yaitu berkemampuan beranak tunggal (A1), kembar dua (A2) dan kembar lebih dari dua (AL2). Paremeter yang diamati, performa ekterior, genetik dan reproduksi. Hasil penelitian menunjukkan bahwa sifat eksterior kualitas induk domba AL2, ekornya tipis 88,25%, muka cembung 52,94%, telinga kecil 82,35% dan tubuh pendek 82,35%. Sifat eksterior kuantitatif induk domba AL2 mempunyai panjang tubuh 63,00+4,94 cm, lebar pinggul 19,88+5,81 cm, panjang tulang ileum 27,59+11,39cm dan lingkar dada 73,29+17,94 cm. Genetik domba AL2 yang memiliki pasangan genetik homozigot CpFCpF dan AmlBAmlB masing-masing mencapai 88,24% sedangkan lokus genetik lainnya berkisar 50,50-69,39%. Berahi setelah beranak domba AL2, 71,7+25,50 hari, lama estrus 18,33+4,81 jam dan jarak beranak 231,60+20,10 hari. Kesimpulan induk domba yang prolifik memiliki penampilan eksterion kualitatif dan kuantitatif, lokus Ceruloplasmin dan Amilase-I, berahi setelah beranak, lama estrus dan interval beranak, sangat berbeda dengan kelompok domba non-prolifik.

Growth Evaluation of Native Chicken on Different Type of Hen Body Weight Sutopo Sutopo; E. Kurnianto; Sutiyono Sutiyono; E. T. Setiatin; Y. S. Ondho; D. A. Lestari; A. Setiaji; A. Suryawijaya; D. Samsudewa
Jurnal Sain Peternakan Indonesia Vol 16, No 4 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.4.292-296

The aim of this research is to measure effect of hen body weight to the growth rate of native chicken. Ten native hens divided by two classifications (heavy and light body weight), 2 native roosters, 58 (from hen with heavy body weight) and 62 (from hen with light body weight) female day old chicks (DoC) use in this research. Hatching machine, communal cage, feeder tray, drinking jar, balance body weight and vernier calliper, were use in this research. Body weight and morphometric of female native chicken from DoC up to 10 week was measured. T-test analysis was used with help of SPSS 25. The results showed that the hen with heavy body weight resulted to the higher morphometric and body weight of female native chicken (P<0.05) on 7 and 8 week, respectively. The growth rate gain of female native chicken hatched by the hen with heavy body weight is significantly difference (P<0.05) on body weight. The conclusion is the hen with heavy body weight will result to the higher body weight and morphometric of female native chicken.

Pengaruh Lama Penyimpanan Telur Tetas Ayam Kedu Jengger Merah terhadap Kualitas Interior Telur U. Latifah; S. Kismiati; Sutopo Sutopo
Jurnal Sain Peternakan Indonesia Vol 15, No 2 (2020)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.15.2.162-166

Penelitian bertujuan untuk mengevaluasi pengaruh lama penyimpanan terhadap kualitas interior telur ayam Kedu jengger merah. Materi yang digunakan adalah 113 butir telur ayam kedu jengger merah. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan. Perlakuan yang digunakan yaitu penyimpanan telur 1 hari (T0), penyimpanan telur 3 hari (T1), penyimpanan telur 5 hari (T2), penyimpanan telur 7 hari (T3) dan penyimpanan telur 9 hari (T4). Parameter yang diamati meliputi bobot putih telur, bobot kuning telur dan haugh unit. Hasil penelitian menunjukkan bahwa lama penyimpanan berpengaruh nyata (p<0,05) terhadap bobot putih telur dan haugh unit, tetapi tidak berpengaruh nyata terhadap bobot kuning telur. Bobot putih telur mulai menurun pada perlakuan T3, sedangkan pada haugh unit menurun pada perlakuan T1. Disimpulkan bahwa semakin lama telur tetas disimpan akan menurunkan kualitas interior.

Keragaman Genetik Itik Magelang Berdasarkan Lebar Kalung Leher Melalui Analisis Protein Plasma Darah di Satuan Kerja Itik Unit Banyubiru Ambarawa N.L. Maulani; Sutopo Sutopo; E. Kurnianto
Jurnal Sain Peternakan Indonesia Vol 11, No 1 (2016)
Publisher : Universitas Bengkulu

Penelitian ini bertujuanuntuk mengetahui tingkat keragaman genetik itik Magelang berdasarkan lebar kalung leher yang berbeda melalui analisis protein plasma darah. Sampel yang digunakan adalah plasma darah 14 ekor itik Magelang kalung sempit dan 14 ekor itik Magelang kalung sedang. Analisis polimorfisme darah yang diamati meliputi lokus Pre-Albumin (Pa), Albumin (Alb), Ceruloplasmin (Cp), Transferrin (Tf), Post-Transferin (P-Tf) dan Amylase-I (Amy-I). Susunan gen hasil elektroforesis digunakan untuk menghitung frekuensi gen. Ragam genetic ditentukan menggunakan rumus heterozigositas individual (h) dan rataan heterozigositas ( ). Pengujian keseimbangan Hardy-Weirnberg dengan perhitungan Chi Square. Hasil penelitian menunjukkan bahwa Lokus Pre-Albumin (Pa), Albumin (Alb), Ceruloplasmin (Cp), Transferin (Tf), Post-Transferin (P-Tf) dan Amylase-I (Amy-I) pada itik Magelang bersifat polimorfik. Lokus Transferin itik Magelang kalung sempit dan lokus Post-Tranferin serta Amylase-I pada itik Magelang kalung sedang memiliki hasil non-signifikan pada taraf pengujian 95% (P>0,05). Hal ini dapat dimaknai bahwa lokus tersebut tidak berada pada kondisi keseimbangan hukum Hardy-Weirnberg. Kata kunci: Itik Magelang, Polimorfisme, Elektroforesis.

Evaluation of Native Rooster Based on Breeding Value of trait of Macroscopic and Microscopic Semen Quality Sutiyono Sutiyono; E. Kurnianto; Sutopo Sutopo; D. K. Ariyanto; D. Samsudewa
Jurnal Sain Peternakan Indonesia Vol 16, No 3 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.3.228-232

The aim of this research is to a determined the breeding value of native roosters based on macroscopic and microscopic semen. Ten native roosters were used in this research. Complete individual cage, semen collection tools and substance, macroscopic and microscopic tools, and substance were used in this research. The native rooster was rested for 2 weeks. Massage techniques were used for semen collection. Parameters observed were volume, pH, color, viscosity, smell, sperm concentration, and sperm livability. The semen collection was repeated 5 times. One-way analysis of variances was used for data analysis with the tools SPSS 16. The breeding value was estimated based on primary traits of semen and calculated for each rooster to make a rank. The results showed that significant parameters (P<0,05) are semen volume and sperm concentration, while the non-significant parameters (P?0,05) are pH semen and sperm livability. Semen color is yellowish-white, semen viscosity is medium-viscous and smell is spermin. The results of breeding value rank are rooster numbers 6, 7, 5, 3, 2, 4, 8, 9, 10, and 1. The conclusion is the breeding value of Native roosters is varied. The recommendation is Native chicken breeding need to apply selection used breeding value based on semen quality.

Gestation Length and Litter Size of New Zealand White Grade Rabbit A. Setiaji; D. A. Lestari; S. Sutopo; Y. S. Ondho; E. Kurnianto
Jurnal Sain Peternakan Indonesia Vol 16, No 3 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.3.288-291

The purpose of this study was to examine performances and factors that influence the gestation length (GL) and litter size (LS) of New Zealand White Grade (NZW Grade) rabbits. Reproduction records of NZW Grade rabbits were taken from April 2020 to March 2021. Data analysis was carried out using the general linear model (GLM) procedure from Statistical Analysis System (SAS) University Edition V.6p.2. software. Farm showed a significant effect (P<0.001) on LS and significant season (P<0.05) on GL and LS. GL in the rainy season (31.56) was shorter than the GL in the summer (32.26), but the LS in the rainy season (7.06) was greater than the LS in the rainy season (6.37). Maintenance management should be improved to reduce the impact of the season on GL and LS NZW Grade rabbits.

Hubungan Genetik antara Domba Wonosobo (Dombos), Domba Ekor Tipis (DET) dan Domba Batur (Dombat) Melalui Analisis Polimorfisme Protein Darah F Noviani; Sutopo Sutopo; E Kurnianto
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 11, No 1 (2013): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.v11i1.4798

The objective of this study was to evaluate the genetic relationships among Wonosobo sheep (Dombos), Thin-Tailed sheep (DET) and Batur sheep (Dombat) through blood protein polymorphism analysis by using electrophoresis Polyacrylamid Gel Electrophoresis-Thin Layer Electrophoresis (PAGE-TLE). The blood of the 20 head of Dombos (Wonosobo regency), 20 head of DET (Semarang regency) and 20 head of Dombat (Banjarnegara regency) were used as materials. Six locus of blood plasma protein were observed, then the gene frequency, individual heterosigosity, averageheterosigosity and genetic distance among three breed were calculated. The results showed that the locus of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I), at DET, Dombos and Dombat were polymorphic (0,380-0,454). In conclusion, the DET and Dombat indicated the closer genetic relationship compared to DET-Dombos and Dombos-Dombat.Key words: sheep, polymorphism, genetic relationship

Phylogenetical Relationships between Kejobong Goat and Other Goats Based on Mt-DNA D-loop Sequence Analysis D. A. Lestari; E. Purbowati; S. Sutopo; E. Kurnianto
Tropical Animal Science Journal Vol. 41 No. 2 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this study was to investigate phylogenetical relationships between Kejobong goat and Kacang goat as well as Etawah Grade goat using genetic diversity, haplotype, and genetic distance analysis based on D-loop sequences. A total of 76 blood samples belonged to three local goats, namely Kacang goat (KC), Etawah Grade goat (EG), and Kejobong goat (KJ). The DNA genome was extracted based on the manufacturer’s standard protocol using gSYNC DNA Mini Kit (Geneaid Biotech Ltd.) for sequence analysis control region (D-loop) in mitochondrial DNA (mtDNA) using specific primer. The results revealed that there were a total of 36 nucleotide substitutions, those were 1 indel (insertion or deletion), 34 haplotypes with Pi = 0.00253±0.00029 and Hd = 0.925±0.018 in three local goats, while intra-breed in this study showed Pi value of EG, KC, and KJ were 0.00452±0.00077, 0.00214±0.00028 and 0.00143±0.00018, respectively and Hd value were 0.985±0.025, 0.926±0.032, and 0.855±0.044, respectively. Genetic distances between KJ and KC, KJ and EG, and between KC and EG were 0.0018; 0.0034, and 0.0036, respectively. The highest NHap was observed in KC (17), followed by EG (15) and KJ (11); and all three local goats were in the same cluster in phylogeny tree. In conclusion, genetically, Kejobong goat is a crossbred of Kacang goat and Etawah Grade goat and has closer genetic relationships to Kacang goat than to Etawah Grade goat.

ANALISIS PENGARUH KUALITAS PELAYANAN ONLINE RESERVATION TICKET TERHADAP KEPUASAN PELANGGAN (Studi Kasus pada PT Kereta Api Indonesia Daop 4 Semarang) Muhammad Ariefian Isnan; Sutopo Sutopo
JURNAL STUDI MANAJEMEN ORGANISASI Vol 11, No 1 (2014)
Publisher : Faculty of Economics and Business Diponegoro University

The background of this research is the accumulation of passengers at ticket counter atdeparture station that indicates a lack of interest in using online reservation ticketservice. This research aims to analyze and provide empirical evidence about the influenceof the four variables of quality electronic services are efficiency (X1), fulfillment (X2),system availability (X3), dan privacy (X4) to customer satisfaction users onlinereservation ticket of PT Kereta Api Indonesia Daop 4 Semarang.Data analysis techniques used in this research is multiple regression analysis. Thepopulation in this study was the passengers that uses the online reservation ticket in thePT Kereta Api Indonesia Daop 4 Semarang. The amount of sampels are 100 respondensand the sampling method is purposive sampling. The data was collected usingquestionnaires.The t test result show that only variables efficiency, fulfillment, and system availabilityhave a positive and significant effect on customer satisfaction .while privacy variable notpositive and significant effect on customer satisfaction. The multiple linier regressionresult show that efficiency variable are independent variables that most influence on thecustomer satisfaction.Keywords: quality of service, efficiency, fulfillment, system availability, privacy, customersatisfaction

Co-Authors A. Febriana A. Febriana A. S. Anggraeni A. Setiaji A. Setiaji A. Setiaji A. Setiaji A. Suryawijaya Agus Tri Sudaryanto Al-Baarri, Ahmad Ni'matullah Asep Setiaji Bhakti Etza Setiani D. A. Lestari D. A. Lestari D. A. Lestari D. A. Lestari D. K. Ariyanto D. Samsudewa D. Samsudewa D. Samsudewa D.R. Dewanti Dela Ayu Lestari, Dela Ayu E Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Kurnianto E. Purbowati E. Purbowati E. Purbowati E. T. Setiatin E.T. Setiatin Edy Kurnianto F Noviani G.D. Brata J. Jiyanto J. Rohyan Julia Ester Lumbantoruan M. F. Harlistyo Muhammad Ariefian Isnan N.L. Maulani R. Ambarwati Rahayu Kusumaningrum S. Johari S. Johari S. Kismiati Sutiyono Sutiyono Sutiyono Sutiyono Sutiyono T. Konno T. Oikawa T. Permatasari U. Latifah W.A. Hamayanti Widiyanto Widiyanto Y. S. Ondho Y. S. Ondho Y.S. Ondho Z. Shujun

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