This study aims to find out the potency of garlic extract (Allium sativum L.) to the growth of Colletotrichum acutatum fungus which cause rot of red chili fruit post-harvest. The treatments were the administration of garlic extract in-vitro and in-vivo. In the in-vitro test, the inhibition of garlic extract was measured using well diffusion method. The in-vivo test was conducted by measuring the diameter area of C. acutatum on red chilies after treatments. The concentration of garlic extract given was 15%(b/v), 20%(b/v), 25%(b/v), 30%(b/v), and 35%(b/v) with positive control of synthetic fungicides and negative control of ethanol solvents 95% and 4 times replications. The average diameter result on the 25%(b/v) concentration of bland power test had the most effective bland zone diameter of 20.1±0.08. Statistically different was real (P?0.05) with negative controls. While in vivo tests the average area of infection area at a concentration of 35%(b/v) had an average diameter area of infection area which was best in inhibiting the growth of C. acutatum fungus.
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