Indonesian Biodiversity Journal
Vol. 1 No. 1 (2020): April 2020

Characteristics Of Partial Mithchondrial Cytochrome Oxidase Sub Unit 1 Gene Of Honey Bee Apis dorsata Binghami [Hymenoptera: Apidae] From Minahasa, North Sulawesi, Indonesia: Characteristics Of Partial Mithchondrial Cytochrome Oxidase Sub Unit 1 Gene Of Honey Bee Apis dorsata Binghami [Hymenoptera: Apidae] From Minahasa, North Sulawesi, Indonesia

Yermia Semuel Mokosuli (Biochemistry and Biomolecular Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, State University of Manado, Indonesia)
Revolson Alexius Mege (Department of Biology, Faculty of Mathematics and Natural Science, State University of Manado, Indonesia)
Jantje Pelealu (Department of Entomology, Graduate School, Sam Ratulangi University, Indonesia)
Max Tulung (Department of Entomology, Graduate School, Sam Ratulangi University, Indonesia)

Article Info

Publish Date
10 Nov 2020

Abstract

The aims of this study were to characterize the mitochondrial cytochrome oxidase subunit 1 gene (CO1) of Apis dorsata from Minahasa, North Sulawesi. Apis dorsata Binghami used in this study was obtained from the natural colony in Klabat forest, Airmadi forest and Kombi forest. From each native bee colony, ten workers were taken (n = 30). Femur hind legs used for subsequent DNA extraction were amplified by PCR, underwent electrophoresis and then sequenced. CO1 sequences then were matched with BLAST to obtain sequences from the NCBI that have high levels of homology (>90%). The results showed that the universal primer LCO -1490 and HCO - 2198 success amplify the gene CO1 Apis dorsata of Minahasa. Based on the phylogeny tree, Apis dorsata derived from forest Airmadidi and forest Kombi have the same CO1 sequences but have had differences with Apis dorsata from forests Klabat. BLAST analysis results showed that the partial mitochondrial CO1 sequences Apis dorsata of Minahasa has the highest rate of 94 % similarity with similar sequences recorded in the NCBI gene bank.

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Journal Info
Indonesian Biodiversity Journal
Website

Abbrev

ibj

Publisher

Universitas Negeri Manado

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Description

International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and ...