Natural B
Vol 2, No 4 (2014)

PFRET Measurements Using Sensitized Emission Methods in Observation of Calcium Changes in Oocytes

Nathania Nanasari (Fakultas MIPA Universitas Brawijaya Jalan Veteran Malang 65145)
Djoko Herry Santjojo (Jurusan Fisika Fakultas MIPA Universitas Brawijaya Malang)
Chomsin Sulistiya Widodo (Jurusan Fisika Fakultas MIPA Universitas Brawijaya Malang)
Hari Soepriandono (Jurusan Biologi Fakultas MIPA Universitas Brawijaya Malang)



Article Info

Publish Date
07 Oct 2014

Abstract

Fluorescence Resonance Energy Transfer (FRET) is a process of non-radiative energy transfer from donor molecule to an acceptor. We expect to know the quantitative FRET measurements in immature and mature goat oocyte with the presence of calcium. FRET was detected using a laser scanning confocal microscope. We assume that calcium as a donor and fluo-3 as an acceptor. The methods of sensitized emission have been used for quantitative FRET measurement. We also describe the precision FRET (PFRET) data analysis. The value of PFRET describe the presence of calsium in immature and mature oocytes. For these immature and mature oocytes, the results of PFRET are 622,5 and 754,57 respectively. Mature oocyte has more calsium than immature oocyte.

Copyrights © 2014






Journal Info

Abbrev

NATURAL B

Publisher

Subject

Environmental Science

Description

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