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All Journal SAINSTIS Physics Student Journal Natural B Berkala Penelitian Hayati
Hari Soepriandono
Jurusan Biologi, FMIPA, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics

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PENGARUH IRRADIASI LASER TERHADAP INTENSITAS FLUORESENSI DARI EKSPERIMEN PENGAMATAN KALSIUM (Ca2+ ) DALAM OOSIT IMMATURE KAMBING YANG DIBERI INDIKATOR FLUO-3 DENGAN MENGGUNAKAN CONFOCAL LASER SCANNING MICROSCOPE (CLSM) rohmaten, siti zulkhijah; Djoko, DJ; Juswono, Unggul P; Soepriandono, Hari
Physics Student Journal Vol 1, No 1 (2013)
Publisher : Department of Physics - Faculty of Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Osilasi kalsium yang terjadi pada oosit yang telah diberi indikator biasanya diamati dengan menggunakan laser fluorescence microscope. Pada mikroskop ini osilasi kalsium dapat terlihat sebagai peristiwa transfer energi yaitu hasil pemendaran warna dari penembakan energi oleh laser ke oosit yang berindikator fluo-3. Penelitian ini bertujuan untuk mengidentifikasi terjadinya peristiwa Fluorescene Resonance Energy Transfer (FRET) dan untuk mengetahui pengaruh dari perubahan pengaturan prosentase laser yang di tembakkan oleh sumber laser yang digunakan sebagai sumber eksitasi pada eksperimen pengamatan Ca2+ dalam oosit kambing dengan indikator fluo-3. Pada penelitian ini menggunakan confocal microscope laser scannning dengan sumber laser Argon 488 nm, laser HeNe G 543nm dan laser HeNe R 633nm. Prosentase laser pada program microscope diatur berubah – ubah tiap lasernya. Analisa terjadinya transfer energi dilakukan dengan membuat grafik puncak - puncak intensitas yang tertangkap oleh channel-channel pada microscope yang membentuk grafik transfer energi. Dari hasil penelitian ini didapatkan bahwa ketika laser yang dinyalakan hanya 1 dengan prosentase yang maksimal, banyaknya kemungkinan transfer energi tidak dapat saling dibandingkan atau data yang didapat random. Sehingga dapat disimpulkan bahwa belum terlihat adanya pengaruh dari dirubahnya prosentase setiap laser pada pengamatan Ca2+ dalam oosit kambing dengan indikator fluo-3.
PFRET Measurements Using Sensitized Emission Methods in Observation of Calcium Changes in Oocytes Nathania Nanasari; Djoko Herry Santjojo; Chomsin Sulistiya Widodo; Hari Soepriandono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.525 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.5

Abstract

Fluorescence Resonance Energy Transfer (FRET) is a process of non-radiative energy transfer from donor molecule to an acceptor. We expect to know the quantitative FRET measurements in immature and mature goat oocyte with the presence of calcium. FRET was detected using a laser scanning confocal microscope. We assume that calcium as a donor and fluo-3 as an acceptor. The methods of sensitized emission have been used for quantitative FRET measurement. We also describe the precision FRET (PFRET) data analysis. The value of PFRET describe the presence of calsium in immature and mature oocytes. For these immature and mature oocytes, the results of PFRET are 622,5 and 754,57 respectively. Mature oocyte has more calsium than immature oocyte.
RESPON PENGGUNAAN BERBAGAI BAHAN AKTIVATOR PADA AKTIVASI PARTENOGENESIS OOSIT KAMBING HASIL IVM Kholifah Holil, Eva Ari Wahyuni, Hari Soepriandono Gatot Ciptadi
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.124 KB) | DOI: 10.18860/sains.v0i0.1860

Abstract

Parthenogenetic activation is one method that can be used to determine the quality of IVM oocytes results before further use to other reproductive technologies (IVF and transfer core). In parthenogenetic activation can be used various activators such as ethanol, Ca Ionophore, and Crude Sperm Extract (CSE). Therefore, the aim of this experiment is to know the response use a variety of materials activator of parthenogenetic activation of goat oocytes IVM.The sample used in this experiment was oocytes aspirated from goat ovarian follicles taken from RPH Sukun of Malang. Oocytes were matured for 24 hr in TCM-199 supplemented with fetal bovine serum (FBS), follicle-stimulating hormone (FSH) and lutheinizing hormone (LH) at a temperature of 38,5oC and 5% CO2 in humidified air. After another 30 hours of in vitro maturation, they were then activated by various treatments. The treatment of experiment are treatment 1, activation using ethanol 7% for 7 minute, treament 2, activation using Ca Ionophore 20 µM for 7 minute. Treatment 3, activation using CSE 2,5 µg/ml for 2 hr.Based on the result of research, it is showed that activation by using 7% ethanol for 7 minutes is able to produce cleavage rate of 70.40%. Activation by Ca Ionophore 20 μM for 7 minutes is able to produce cleavage rate of 52.75%. While the use of CSE activation with 2.5 ug / ml for 2 hours produces cleavage rate of 36.33%. Thus it can be concluded that the goat oocyte IVM able to respond to a variety of materials activators on parthenogenetic activation performed. The highest response given by the successive results goat IVM oocytes activated using 7% ethanol for 7 minutes, 20 μM Ca Ionophore for 7 minutes, and the CSE 2.5 microg / ml for 2 hours.Keywords: Parthenogenetic activation, goat oocytes IVM, etanol, calsium ionophore, Crude Sperm Extract (CSE).
AMBANG WAKTU PENDEDAHAN TELUR Lymnaea sp UNTUK MENGAMATI ABNORMALITAS EMBRIO SEBAGAI PARAMETER DALAM UJI HAYATI TERHADAP KARBAMAT Hari Soepriandono; Bambang Irawan; Sri Puji Astuti Wahyuningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 1 (2000): December 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/488

Abstract

The aim of this study was to know the specific expose period of the Lymnaea sp.’s embyos and their age in order to utilization eggs of Lymnaea sp. in bioassay. Fifty individuals of Lymnaea sp. collected from Gonggan River Magetan were captured in laboratory. Egg cluster of each individual then was placed in petry dish which was filled by Baycarb 500 EC of 0.0001, 0.001 and 0.01 ppm. The result showed that 12 hours exposure in Baycarb 500 EC was the effective time since within 6 hours all of embryos were death and abnormalities of the survivals was detected in the embryos of 48 and 72 hours.
Co-Authors Bambang Irawan Chomsin Sulistiya Widodo D. J. Djoko Herry Santjojo DJ Djoko Nathania Nanasari siti zulkhijah rohmaten Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Unggul P Juswono